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A kind of detection method of cosmetic allergen and allergen patch kit

A patch and reagent technology, applied in the field of compound detection, can solve the problems of cumbersome allergen screening methods, inaccurate test results, long detection time, etc., achieve good water solubility, shorten detection time, and short detection time

Active Publication Date: 2021-11-16
黑龙江省医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a detection method for cosmetic allergens and an allergen patch kit, the main purpose of which is to overcome the defects of cumbersome screening methods for cosmetic allergens, low sensitivity, long detection time, skin irritation and inaccurate detection results.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Step 1. Select 300 ACD volunteers;

[0047] Step 2. Prepare 100 copies of patch kits of the present invention containing different concentrations (including methylisothiazolinone: 0.01%, 0.02%, 0.03%), and coat them on the left forearm of the volunteers respectively. area skin;

[0048] The left forearm patch kit includes the following components by mass percentage:

[0049] Polyethylene Glycol 6000 75%; Xanthan Gum 1%; Sodium Alginate 1%;

[0050] Glycerin 20%;

[0051] Buffer (sodium chloride: potassium dihydrogen phosphate: disodium hydrogen phosphate mass ratio is 14:1:20) 0.6%; balance water

[0052] Step 3. Prepare the ordinary patch control sample. The preparation method: add 2% glycerin, 1% acrylic resin, heat to 65°C, and adjust the pH to 6, stir, add methylisothiazolinone (0.01%, 0.02%, 0.03%) each 100 parts, add one by one in the small groove of common patch, shape, wrap on the tested area skin of volunteer's right forearm respectively;

[0053] Step 4: ...

Embodiment 2

[0059] Step 1. Select 300 ACD volunteers;

[0060] Step 2, each 100 copies of the prepared patch kits of the present invention containing different concentrations (including methylchloroisothiazolinone: 0.1%, 0.2%, 0.3%) were coated on the left forearm of the volunteer respectively. test area skin;

[0061] The patch kit comprises the following components by mass percentage:

[0062] Gelatin 45%; Carbomer 1%; Tragacanth 1%;

[0063] Propylene Glycol 10%; Glycerin 10%;

[0064] Buffer (sodium chloride: potassium dihydrogen phosphate: disodium hydrogen phosphate mass ratio is 14:1:20) 0.6%; balance water

[0065] Step 3. Prepare the ordinary patch control sample. Preparation method: Soak water and aristoflex AVC 1%, swell, disperse, add pentylene glycol 3%, stir evenly, adjust pH to 6, add polyurethane 6%, stir evenly, add formaldehyde Each 100 parts of isothiazolinone (0.01%, 0.02%, 0.03%) was added one by one in the small groove of the common patch, shaped, and respectivel...

Embodiment 3

[0072] Step 1. Select 100 ACD volunteers;

[0073] In step 2, the prepared patch kit containing 1% cinnamaldehyde is coated on the skin of the test area of ​​the left forearm of the volunteer; the patch kit includes the following components by mass percentage:

[0074] Polyvinylpyrrolidone 60%; Xanthan Gum 1%; Sodium Alginate 1%; Chitosan 1%;

[0075] Menthol 28%;

[0076] Buffer (sodium chloride: potassium dihydrogen phosphate: disodium hydrogen phosphate mass ratio is 14:1:20) 0.5%; balance water

[0077] Matters needing attention are the same as in Embodiment 1.

[0078] Step 3. Prepare the ordinary patch control sample. The preparation method: soak and swell water and 1% acrylic resin cross-linked polymer, disperse, add 3% pentylene glycol and stir evenly, adjust the pH to 6, add 5% gum arabic, Stir evenly, add 1% cinnamaldehyde, add one by one into the small grooves of the ordinary patch, shape, and wrap the skin of the test area on the right forearm of the volunteer; ...

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Abstract

The invention provides a method for detecting cosmetic allergens and an allergen patch kit. The detection method includes the following steps: a) placing the cosmetic to be tested in the patch kit, and the cosmetic to be tested contains allergens: Spices, preservatives, phenylenediamines; the patch ingredients in the patch kit include the following components according to the mass percentage of the composition: matrix 1%-90%; absorption-promoting agent 1-30%; solvent 0 -70%; buffer solution 0.1-1%; b) clean the skin at the test point area; c) wrap the patch kit that has been loaded with the sample solution obtained in step a on the skin at the clean test point area in step b; d) Obtain and analyze the digital image of the skin after patch treatment; the invention is easy to operate, short in detection time, higher in sensitivity and accuracy, does not pollute clothes when removed, and is more suitable for individualized application of beauty seekers when screening cosmetics.

Description

technical field [0001] The invention belongs to the technical field of compound detection, and mainly relates to a detection method of a cosmetic allergen and an allergen patch kit. Background technique [0002] Allergic contact dermatitis (ACD) is a cell-mediated hypersensitivity reaction caused by contact with allergens, and is the most common clinical category of contact dermatitis (CD). Allergic contact dermatitis is a typical type IV hypersensitivity reaction, and the contact is a sensitizing factor. Contact objects can act as antigens to initiate an immune response at the site of skin contact. When exposed to the hapten again, the incubation period from the exposure to the appearance of the rash will be shorter than the first time. At this time, the sensitizing factor still needs to form the hapten first, due to the action of the specifically sensitized T lymphocytes, generally within 24-48 hours produce a marked inflammatory response. Common clinical symptoms inclu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
CPCA61K49/0006
Inventor 阿荣娜赵英马国章王鹤飞柳曦光
Owner 黑龙江省医院
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