Inducer for inducing mesenchymal stem cells to differentiate into islet cells
A technology for inducing differentiation and islet cells, applied in the field of biomedicine, can solve the problems of unclear induction mechanism, low efficiency of induction differentiation, complicated induction process, etc., and achieve the effect of no ethical issues, few induction steps, and short induction time
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Embodiment 1
[0017] Example 1 The inducer for inducing mesenchymal stem cells into islet cells in this example is composed of the following components in mass concentration ratio: GLP-1 30 mg / L, parathyroid hormone 9 mg / L, acetaminophen 5mg / L, rapamycin 5mg / L, icariin 5mg / L, trametinib 0.4mg / L, EPO 3ug / L, VEGF 3ug / L. Add the above components to human mesenchymal stem cell serum-free medium (or DMEM+10%FBS or other types of commercially available mesenchymal stem cell medium) in sequence according to the mass concentration ratio, mix well, and filter to sterilize.
[0018] The components of the inducer of the present invention are all commercially available products: human mesenchymal stem cell serum-free medium, brand LONZA, product number 00190632; GLP-1 (glucagon-like peptide 1), brand Sigma, product number scp0153; parathyroid gland Hormone, brand Sigma, product number P7036; acetaminophen, Shanghai Yiji Industrial Co., Ltd., product number Y903952; rapamycin, brand TargetMol, product n...
Embodiment 2
[0020] The inducer for inducing mesenchymal stem cells into islet cells in this example is composed of the following components in mass concentration ratio: GLP-1 20 mg / L, parathyroid hormone 6 mg / L, acetaminophen 2 mg / L , Rapamycin 2mg / L, Icariin 2mg / L, Trametinib 0.3mg / L, EPO 2ug / L, VEGF 2ug / L. Add the above components into human mesenchymal stem cell serum-free medium (or DMEM+10%FBS) in sequence according to the mass concentration ratio, mix well, and filter to sterilize.
Embodiment 3
[0022] The inducer for inducing mesenchymal stem cells into islet cells in this example is composed of the following components in mass concentration ratio: GLP-1 40mg / L, parathyroid hormone 12mg / L, acetaminophen 8mg / L , Rapamycin 8mg / L, Icariin 8mg / L, Trametinib 0.6mg / L, EPO 4ug / L, VEGF 4ug / L. Add the above components into human mesenchymal stem cell serum-free medium (or DMEM+10%FBS) in sequence according to the mass concentration ratio, mix well, and filter to sterilize.
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