Inducer for inducing mesenchymal stem cells to differentiate into islet cells

A technology for inducing differentiation and islet cells, applied in the field of biomedicine, can solve the problems of unclear induction mechanism, low efficiency of induction differentiation, complicated induction process, etc., and achieve the effect of no ethical issues, few induction steps, and short induction time

Active Publication Date: 2021-07-13
QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The induction and differentiation conditions of each laboratory are different, the induction and differentiation mechanism is not yet clear, the induction and differentiation efficiency is low, the islet secretion capacity is only about 1% of normal islets, and the induction process is complicated, the induction time is long, the number of cells obtained is small, and the function is low

Method used

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  • Inducer for inducing mesenchymal stem cells to differentiate into islet cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 The inducer for inducing mesenchymal stem cells into islet cells in this example is composed of the following components in mass concentration ratio: GLP-1 30 mg / L, parathyroid hormone 9 mg / L, acetaminophen 5mg / L, rapamycin 5mg / L, icariin 5mg / L, trametinib 0.4mg / L, EPO 3ug / L, VEGF 3ug / L. Add the above components to human mesenchymal stem cell serum-free medium (or DMEM+10%FBS or other types of commercially available mesenchymal stem cell medium) in sequence according to the mass concentration ratio, mix well, and filter to sterilize.

[0018] The components of the inducer of the present invention are all commercially available products: human mesenchymal stem cell serum-free medium, brand LONZA, product number 00190632; GLP-1 (glucagon-like peptide 1), brand Sigma, product number scp0153; parathyroid gland Hormone, brand Sigma, product number P7036; acetaminophen, Shanghai Yiji Industrial Co., Ltd., product number Y903952; rapamycin, brand TargetMol, product n...

Embodiment 2

[0020] The inducer for inducing mesenchymal stem cells into islet cells in this example is composed of the following components in mass concentration ratio: GLP-1 20 mg / L, parathyroid hormone 6 mg / L, acetaminophen 2 mg / L , Rapamycin 2mg / L, Icariin 2mg / L, Trametinib 0.3mg / L, EPO 2ug / L, VEGF 2ug / L. Add the above components into human mesenchymal stem cell serum-free medium (or DMEM+10%FBS) in sequence according to the mass concentration ratio, mix well, and filter to sterilize.

Embodiment 3

[0022] The inducer for inducing mesenchymal stem cells into islet cells in this example is composed of the following components in mass concentration ratio: GLP-1 40mg / L, parathyroid hormone 12mg / L, acetaminophen 8mg / L , Rapamycin 8mg / L, Icariin 8mg / L, Trametinib 0.6mg / L, EPO 4ug / L, VEGF 4ug / L. Add the above components into human mesenchymal stem cell serum-free medium (or DMEM+10%FBS) in sequence according to the mass concentration ratio, mix well, and filter to sterilize.

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Abstract

The invention belongs to the field of biomedicine, and relates to an inducer for inducing mesenchymal stem cells to differentiate into islet cells. The invention discloses the inducer for inducing mesenchymal stem cells to be differentiated into islet cells. The inducer is prepared from the following components: GLP-1, parathyroid hormone, acetaminophen, rapamycin, icariin, trametinib, EPO and VEGF. According to the inducer for inducing and differentiating the mesenchymal stem cells into the islet cells, all the components are safe and non-toxic, the number of steps needed by induced differentiation is small, the time is short, and the induction efficiency is high.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to an inducer for inducing differentiation of mesenchymal stem cells into islet cells. Background technique [0002] Diabetes mellitus (DM) is an endocrine and metabolic disease characterized by glucose and lipid metabolism disorders. According to the forecast released by the World Health Organization and the International Diabetes Federation, by 2030, there will be 370 million people with diabetes worldwide. At present, the number of DM patients in my country has exceeded 45 million, ranking second only to India. Although their etiologies are different, they all manifest as defects in the number and function of pancreatic islet cells, and ultimately require the use of insulin for treatment. Although due to the application of insulin, especially in recent years, some progress has been made in insulin dosage forms and administration routes, but practice has proved that exogenous insulin can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
CPCC12N5/0676C12N2506/1346C12N2501/335C12N2501/37C12N2501/165C12N2501/14C12N2500/30C12N2501/999C12N2506/1353C12N2501/395C12N2506/1369C12N2501/30
Inventor 张炳强陈梦梦李翠翠王二朴王福斌邹伟付学奇刘翠娟
Owner QINGDAO RESTORE BIOTECHNOLOGY CO LTD
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