A serum-free complete medium for inducing mesenchymal stem cells to differentiate into corneal epithelial cells

Abstract

The invention relates to the field of induced differentiation of stem cells, in particular to a serum-free complete medium for inducing mesenchymal stem cells to differentiate into corneal epithelial cells, prepared by the following method: every 1 L of the serum-free complete medium for induced differentiation contains resveratrol Alcohol 5-10μmol, Icariin 2-4μmol, Aspirin 1-3nmol, Parathyroid hormone 1-3nmol, Hydrocortisone 5-10nmol, Rapamycin 1-3mg, Testosterone 2-10μg, EPO 2- 10μg, LIF 2-10μg, the balance is serum-free medium for corneal epithelial cells, and it can be filtered and sterilized after mixing. The serum-free complete medium for inducing the differentiation of mesenchymal stem cells into corneal epithelial cells of the present invention adopts traditional Chinese medicine ingredients resveratrol, icariin combined with aspirin, parathyroid hormone, hydrocortisone, rapamycin hormone, testosterone and growth factors, synergistically induce the directional differentiation of mesenchymal stem cells into corneal epithelial cells. The selected induction components are non-toxic, with high induction efficiency, short induction time, good activity of induced corneal epithelial cells, and no rejection after cell transplantation. , no ethical issues, high security.

Description

technical field [0001] The invention relates to the field of stem cell induction and differentiation, in particular to a serum-free complete medium for inducing the differentiation of mesenchymal stem cells into corneal epithelial cells. Background technique [0002] The corneal epithelial cell layer is located on the outer surface of the cornea. There are 5 to 6 layers of cells in the upper layer of the cornea, each layer is very neat and compact, the deepest layer is a single layer of short columnar basal cells; the deep layer has 2 to 3 layers of polygonal wing cells, and the uppermost layer is 2 to 3 layers of polygonal surface layer cell. The most superficial layer of epidermal cells is non-keratinized flat cells, the gap between epidermal cells is not clear, the superficial cell membrane is very smooth, the cytoplasm is clear and transparent, the cytoplasmic protrusions combine with each other to form a bridge, and the nucleus still exists. This cell is very transpar...

AI Technical Summary

Problems solved by technology

However, only about 10,000 cases of corneal transplantation can be carried out in my country every year. The main reason is that the number of donated corneas is seriously insufficient, which limits the popularization and application of the surgery, resulting in the vast majority of patients with corneal blindness unable to regain their sight through corneal transplantation. Reconstruction of tissue engineered cornea is an important breakthrough to solve the shortage of donor cornea materials

[0004] However, there are currently some bottlenecks in corneal tissue engineering, such as the source of corneal epithelial seed cells.

The study of corneal epithelium has enabled people to have a deeper understanding of the physiological and pathological characteristics of the cornea and corneal diseases. However, due to the short life cycle of the differentiated corneal epithelial cells in vitro, they can only be passed on for 2 to 3 generations. The quantity is small and the cost is high, which limits the research of corneal tissue and the construction of tissue engineered cornea

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