Immune cell capable of automatically secreting IL-15 and anti-PD1 fusion protein

Abstract

The invention relates to the technical field of biological cells, in particular to an immune cell capable of automatically secreting IL-15 and anti-PD1 fusion protein, and the immune cell subjected to gene editing is used for secreting the IL-15 and anti-PD1 fusion protein so as to improve the activity of the immune cell and the killing effect on tumors. The immune cell capable of automatically secreting IL-15 and anti-PD1 fusion protein comprises the fusion protein is expressed in series according to the sequence of anti-PD1, G4S * 4 Linker, IL-15N72D, G4S * 4 Linker and IL-15RaSu, and the cell used for expressing the fusion protein and influenced by the autosecretion fusion protein and subjected to gene editing. According to the invention, the IL-15 and IL15Ra super kinetin and the anti-PD1 fusion protein are combined, and the CART cells successfully secrete the fusion protein, so that the proliferation ability, the anti-apoptosis ability and the tumor killing ability of the CarT cells are enhanced, the killing effect is accurate, the safety is higher, the tumor is not easy to relapse, and the life quality of patients is improved.

Description

technical field [0001] The present invention relates to the technical field of immune cells of autocrine fusion proteins, in particular to immune cells expressing chimeric antigen receptors, in particular to immune cells of autocrine IL-15 and anti-PD1 fusion proteins. Background technique [0002] Tumor (tumour) refers to the neogrowth formed by the proliferation of local histiocytes under the action of various tumorigenic factors, because most of these neogrowths are space-occupying block-like protrusions, also known as neoplasms (neoplasm) . Among them, malignant tumors are extremely difficult to cure due to their susceptibility to metastasis, relapse after treatment and their special microenvironment. [0003] IL-15 plays a crucial role in the development, homeostasis, and function of T cells, NK cells, and NK cells, and also has various effects on B cells, dendritic cells (DCs), macrophages, and mast cells. function. IL-15 is a member of the cytokine 4-α-helix bund...

AI Technical Summary

Problems solved by technology

Among them, malignant tumors are extremely difficult to cure due to their susceptibility to metastasis, relapse after treatment, and their special microenvironment.

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