Characterization and application of novel high-temperature Argonaute protein

A technology of reaction and reaction system, applied in the field of molecular biology and biology, can solve the problems of cumbersome steps and high detection cost

CN113106087AActive Publication Date: 2021-07-13SHANGHAI JIAO TONG UNIV

2 Cites 4 Cited by

Patent Information

Authority / Receiving Office: CN · China
Current Assignee / Owner: SHANGHAI JIAO TONG UNIV
Publication Date: 2021-07-13

Smart Images

Figure 1
Figure 2
Figure 3

Patent Text Reader

Abstract

The invention provides characterization and application of a novel high-temperature Argonaute protein. Specifically, the invention provides a nucleic acid cleavage system, which is characterized in that the nucleic acid cleavage system comprises: (a) guide DNA (gDNA); (b) a programmable endonuclease Argonaute (Ago); and (c) optionally a reporter nucleic acid wherein if the reporter nucleic acid is cleaved, the cleavage can be detected. In addition, the invention also provides a system and a method for enriching and detecting low-abundance target nucleic acid based on the programmable endonuclease Ago provided by the invention. The detection system and method provided by the invention can specifically and effectively enrich and efficiently detect low-abundance nucleic acid, and the programmable endonuclease Ago provided by the invention has strong gene manipulation potential.

Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of molecular biology and biotechnology, and specifically relates to the characterization and application of a novel high-temperature Argonaute protein. Background technique

[0002] The Argonaute (Ago) protein was first mentioned in a study describing a mutant of Arabidopsis thaliana. Ago proteins are key players in the eukaryotic RNA interference (RNAi) pathway that regulate gene expression post-transcriptionally, thereby defending against host-invading RNA viruses and preserving genome integrity. The reported Ago proteins are mainly divided into two types: eukaryotic Ago (eAgo) and prokaryotic Ago (pAgo).

[0003] Prokaryotic Ago can bind to single-stranded guide DNA or RNA, and catalyze the cleavage of target DNA or RNA that is complementary to guide. Different from the CRISPR / Cas system, prokaryotic Ago does not need a PAM sequence when cutting the target nucleic acid chain. It can bind to the guide (DNA or RNA)...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

Login to View More