Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lactobacillus bb1 enhancing memory ability and its application

A Lactobacillus, BB1 technology, applied in the field of microorganisms, can solve the problem of few types of probiotics, and achieve the effect of improving memory impairment, broad application prospects, and improving long-term memory ability

Active Publication Date: 2022-08-02
JIANGNAN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is known that the functions of probiotics are mainly to regulate intestinal function, improve host nutrient metabolism, etc., but there are very few known types of probiotics that can improve cognitive impairment
In addition, no studies have reported that a specific probiotic improves cognitive performance in healthy individuals

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lactobacillus bb1 enhancing memory ability and its application
  • Lactobacillus bb1 enhancing memory ability and its application
  • Lactobacillus bb1 enhancing memory ability and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Screening and identification of strains

[0028] (1) Isolation and screening of Lactobacillus:

[0029] (1) The hindgut tissues of 3 healthy bumblebees were taken and ground into a homogenate. After gradient dilution, it was spread on MRS solid medium and cultured at 37°C for 72 hours in an anaerobic environment;

[0030] (2) Observe and record the colony morphology, pick colonies for streak purification;

[0031] (3) Pick a single colony, culture it in MRS liquid medium at 37°C for 48 hours and collect the bacteria for strain identification.

[0032] (2) Molecular biological identification of Lactobacillus:

[0033] (l) Use E.Z.N.A. Stool DNA kit (Omega Bio-tek) to extract single bacterial genomic DNA;

[0034] (2) Amplify 16S rDNA using universal primers for bacterial species identification;

[0035] (3) Prepare a 1% agarose gel, mix the PCR product with the loading buffer and load the sample, run at 150 V and 100 mA for 20 min, observe and cut the gel;...

Embodiment 2

[0038] Example 2 The effect of Lactobacillus BB1 on the memory ability of bumblebee individuals

[0039] The activated Lactobacillus BB1 was cultured anaerobic in MRS liquid medium at 37°C for 48 hours; Gilliamella apicola Activated and inoculated in LB liquid medium, enriched with CO at 37°C 2 (6%) for 48 hours. The fresh stationary phase bacterial culture medium was centrifuged, and after washing with PBS, the bacterial pellet was dissolved in 40% (w / w) sucrose water, so that the bacterial concentration reached OD600 = 1.0.

[0040] The newly released worker bees were marked every day, and when they were 3 days old, they were transferred to different small wooden boxes for group feeding. There were 3 groups in total, with 15 bees in each group. The specific grouping conditions were as follows: (1) Control group: each group Provide 40% (w / w) sucrose water every day; (2) Lactobacillus BB1 feeding group: provide 40% sucrose water containing Lactobacillus BB1 (OD600=1) freshl...

Embodiment 3

[0043] Example 3 Effects of Lactobacillus BB1 on the metabolism and content of glycerophospholipids in the intestinal tract

[0044] The bumblebees of the control group and the Lactobacillus BB1 feeding group in Example 2 were placed on dry ice to suffocate, and the hindgut tissue, hemolymph and brain tissue of the bumblebee were dissected and extracted. The tissue samples were ground with liquid nitrogen and placed in EP tubes, respectively, and 80% methanol aqueous solution containing 0.1% formic acid was added. After vortexing, let it stand in an ice bath for 5 minutes, centrifuge at 15,000 g for 10 minutes at 4°C, take a certain amount of supernatant, and add mass-grade water to dilute to 53% methanol. Placed in a centrifuge tube at 15,000 g and centrifuged at 4°C for 10 minutes, collected the supernatant, and injected into LC-MS for analysis.

[0045] The results showed that feeding Lactobacillus BB1 significantly altered gut, hemolymph and brain metabolites ( figure 2...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a memory-enhancing lactobacillus BB1 and an application thereof, belonging to the technical field of microorganisms. The lactobacillus BB1 of the present invention can be used to prepare medicines for improving the memory ability of healthy individuals and improving memory impairment.

Description

technical field [0001] The invention relates to Lactobacillus BB1 for enhancing memory ability and application thereof, and belongs to the technical field of microorganisms. Background technique [0002] Learning and memory are the basis of human cognition, and also the functions that individuals must have to adapt to the social environment to survive better. Many factors, such as lack of sleep, stress, aging and disease, can cause the decline of learning and memory ability. Severe learning and memory impairment will not only affect the individual's life and social adaptability, but also bring heavy pressure and economic burden to the family and society. At present, the effects of educational functional products aimed at improving the learning and memory ability of infants, adolescents, adults and the elderly are very limited, especially for memory impairment caused by neurodegenerative diseases such as Alzheimer's There is no effective treatment. [0003] The intestinal fl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A23L29/00A23L19/00A23L11/50A23C9/123A23C13/16A23C19/06A23K10/12A61K35/747A61P25/28C12R1/225
CPCC12N1/20A23L33/135A23L29/065A23L19/00A23C9/123A23C13/16A23C19/061A23K10/12A61K35/747A61P25/28A23V2002/00A23V2400/11A23V2200/322A23L11/50A23K10/18C12N1/205C12R2001/225A23V2200/3204
Inventor 赵伟齐昭阳李莉
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com