High-concentration sample detection and time sequence calling method

Abstract

The invention discloses a high-concentration sample detection and time sequence calling method and device, electronic equipment and a medium. The method comprises the steps: sucking a first sub-sample with a preset dose from a first to-be-detected sample; taking the first sub-sample as a current sample, and obtaining a sample suction frequency corresponding to the current sample; determining a target dosage of an added reagent corresponding to the current sample according to a corresponding relationship between a preset number of sample suction times and a dosage ratio of the added reagent; adding the current sample and the adding reagent of the target dosage into a reaction cup, and mixing to obtain a current diluted sample; performing detection operation on the current diluted sample to obtain a concentration detection result of the current diluted sample; and determining whether the current diluted sample needs to be diluted or not according to the concentration detection result.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a method, device, electronic equipment and medium for the detection and timing call of high-concentration samples. Background technique [0002] At present, the detection method of specific protein generally adopts latex-enhanced immunoscattering turbidimetry. After the latex particle absorbs the antibody, it will produce an agglutination reaction to form an antigen-antibody conjugate when it encounters an antigen, resulting in an increase in the concentration of the solution. This concentration change is directly proportional to the amount of antigen detected, and then the optical component measures the intensity of scattered light caused by the conjugate in the cuvette, and compares it with the reaction concentration calibration curve generated by a standard of known antigen concentration. Estimate the antigen concentration value of the first sample to be tested....

AI Technical Summary

Problems solved by technology

[0003] In this technique, since the amount of antibody (ie latex) has been determined, but the concentration of the first sample to be tested cannot be determined, there will be some samples with too high antigen concentration, and the detected result value will be low instead. This phenomenon is the hook effect or HOOK effect

Due to the existence of the HOOK effect, the tested samples cannot be correctly distinguished, and it is impossible to judge whether the concentration exceeds the linear range of the detection kit or the concentration itself is the value, so that misdiagnosis may occur

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