A class of active peptides derived from caprin1 and their applications

An active peptide and active technology, applied in the fields of application, animal/human peptides, medical preparations containing active ingredients, etc.

Active Publication Date: 2022-07-12
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether the anticancer activity of the above small molecule compounds through the inhibition of SGs protein is dependent on the inhibition of SGs remains to be further studied to reveal

Method used

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  • A class of active peptides derived from caprin1 and their applications
  • A class of active peptides derived from caprin1 and their applications
  • A class of active peptides derived from caprin1 and their applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Design of polypeptide SGs inhibitor based on blocking the interaction between Caprin1 and G3BP1 1. According to research reports, the interaction between Caprin1 and G3BP1, two nucleating proteins of SGs, can promote the assembly of SGs. The present invention explores the minimal G3BP1 binding region of Caprin1 protein, and designs four polypeptide fragments according to Caprin1 protein: Caprin1 (258-415), Caprin1 (301-390), Caprin1 (301-350), Caprin1 (351-390). Shanghai Jill Biochemical Company was entrusted to synthesize the above-mentioned polypeptide fragments.

[0043] 2. Construct Caprin1 and hG3BP1 expression plasmids, design and synthesize the following primers:

[0044] G3BP1:

[0045] G3BP1-F 5'-atggtgatggagaagcctagtcccctgct-3' (SEQ ID No: 14);

[0046] G3BP1-Xba-R 5'-ccatctagattcactgccgtggcgcaagcc-3' (SEQ ID No: 15);

[0047] Caprin1:

[0048] Caprin1-F 5'-atgccctcggccaccagccaca-3' (SEQ ID No: 16);

[0049] Caprin1-Xba-R 5'-tcttctagaaacatattatg...

Embodiment 2

[0057] Example 2 Investigating the ability of the active peptide derived from Caprin1 of the present invention to compete with the protein Caprin1 for binding to G3BP1

[0058] Based on the research results in Example 1, Caprin1 (361-385) with a shorter sequence was designed on the basis of Caprin1 (351-390).

[0059] 1. Construction of Caprin1 (361-385) and Caprin1 (351-390) expression plasmids

[0060] Design and synthesize the following primers:

[0061] Caprin1 (351-390):

[0062] Caprin1-351F 5'-gatccccttgtgagaagacagcgagtacaa-3' (SEQ ID No: 18);

[0063] Caprin1-Xba-390R 5'-ctgtctagatacaatggcaggatcaagtgt-3' (SEQ ID No: 19);

[0064] Caprin1 (361-385):

[0065] Caprin1-361F 5'-gaccttatggcacaaatgcagggtccctat-3' (SEQ ID No: 20);

[0066] Caprin1-Xba-385R 5'-tgatctagaaagtgtctgattttcaaaatc-3' (SEQ ID No: 21).

[0067] With reference to "Molecular Cloning Experiment Guide (Fourth Edition)" (Cold Spring Harbor Laboratory Press / Science Press), the pCS2-Myc-hCaprin...

Embodiment 3

[0082] Example 3 Investigating the activity of Caprin1 (361-385) and Caprin1 (351-390) in inhibiting SGs HeLa cell lines were seeded in 6-well plates, and when the cell confluence reached about 60%, the expression Caprin1 constructed in Example 2 was (361-385) and Caprin1 (351-390) plasmids were transfected into HeLa cells and overexpressed in the cells. When the cell confluence reaches about 80%, take 12 mL of cell culture medium into a 15 mL centrifuge tube, add 12 μL of 0.5 mM AS, mix well, replace the medium in the 6-well plate, and stimulate HeLa cells to form SGs (using endogenous G3BP1 as the SGs). Mark). Put into a carbon dioxide cell incubator and continue to culture for 45min. Remove the 6-well plate for immunofluorescence experiments.

[0083] The result is as image 3 As shown, the results showed that overexpression of Caprin1(351-390), Caprin1(361-385) inhibited AS-induced SGs ( image 3 A). In order to ensure the authenticity and reproducibility of the exper...

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Abstract

The present invention designs a class of active peptides derived from Caprin1, which can competitively bind to G3BP1 protein, specifically block the interaction between Caprin1 and G3BP1 protein, inhibit the generation of stress granules, and then treat diseases related to the generation of SGs, such as tumor or neurodegenerative disease.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to a class of active peptides derived from Caprin1, which can compete with Caprin1 to bind G3BP1 / 2, inhibit the assembly of stress granules, and produce corresponding biological effects. Background technique [0002] There are many membraneless structures rich in RNA and proteins in cells, called ribonucleoproteins (RNPs). These RNP particles are individual organelles composed of thousands of molecules that are ubiquitous in cells. When eukaryotic cells respond to stress stimuli such as endoplasmic reticulum stress, heat shock, hypoxia, and arsenate, they form aggregated granules of mRNA-protein complexes in the cytoplasm, namely stress granules (stress granules, SGs). [0003] The formation of SGs has important physiological significance, including through the regulation of mRNA translation and inhibition of apoptosis-related signaling pathways and other mechanisms, to minim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47C07K19/00C12N15/12A61K38/17A61P35/00A61P9/10A61P25/00A61P25/08A61P25/28A61P25/16A61P25/14
CPCC07K14/4702A61P35/00A61P9/10A61P25/00A61P25/08A61P25/28A61P25/16A61P25/14C07K2319/10A61K38/00
Inventor 金志刚李娟周钰林金博星李晨辉
Owner ZHEJIANG NORMAL UNIVERSITY
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