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Gellan gum with low gelation temperature as well as preparation method and application thereof

A gellan gum and low-gel technology, applied in the field of gellan gum, can solve the problem that the gel temperature hinders the application, and achieve the effect of convenient gel temperature

Pending Publication Date: 2021-07-23
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its high gel temperature hinders its application in medical science and other fields, and its gel temperature is much higher than 37°C of body temperature.

Method used

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  • Gellan gum with low gelation temperature as well as preparation method and application thereof
  • Gellan gum with low gelation temperature as well as preparation method and application thereof
  • Gellan gum with low gelation temperature as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of gellan gum with a low gel temperature, comprising the steps of:

[0025] (1) At 20-30°C, add the carboxyl activator EDC or NHS to the gellan gum aqueous solution of a certain concentration within the range of 0.1:1 to 1:1 in mass ratio to gellan gum (the concentration range is 0.5%~1.5% (w / v)), the reaction lasts for more than 1h, and the carboxyl group in gellan gum is activated.

[0026] (2) Add a certain amount of glutathione (glutathione: gellan gum mass ratio must be greater than 0.25) for reaction, and adjust the pH to 5.0-6.0, and the reaction lasts for 3±0.5h. After the reaction is finished, the obtained product is put into a dialysis bag (molecular weight cut off is 12-14kDa) for dialysis purification. The dialysate was 1mM HCl aqueous solution, and the N 2 Then place it at 4±4°C for dialysis to prevent the oxidation of sulfhydryl groups during the purification process. During the dialysis process, a DTNB test machine was used to meas...

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Abstract

The invention belongs to the technical field of gellan gum, and particularly relates to gellan gum with low gelation temperature as well as a preparation method and application of the gellan gum. Gellan gum is modified by glutathione, so that the gelation temperature of the gellan gum is obviously reduced. When the mass concentration is 1% and 1.5%, the gel temperature of the gellan gum solution with low gelation temperature is reduced to 18.0 DEG C and 22.1 DEG C from 34.6 DEG C and 44.0 DEG C before modification respectively when the gelation temperature of the gellan gum solution with low gelation temperature is compared with that of an unmodified gellan gum solution (pH = 6); the gellan gum with the low gelation temperature can be more conveniently applied to the fields of drug delivery systems, tissue engineering materials and the like due to the low gelation temperature of the gellan gum.

Description

technical field [0001] The invention belongs to the technical field of gellan gum, relates to gel temperature modification of gellan gum, in particular to gellan gum with low gel temperature, a preparation method and application thereof. Background technique [0002] Gellan gum is a linear polyanionic microbial polysaccharide with unique gel properties and solution rheology. In recent years, with the development of biomedical sciences, natural polymer gellan gum and its temperature-responsive gel have shown broad application prospects in the fields of drug delivery systems and tissue engineering materials. Gellan gum is non-toxic, biocompatible and biodegradable, and the temperature-responsive gel formed is transparent and stable, and the mechanical properties of the gel are similar to those of ordinary human tissues under certain conditions. Gellan gum is an important excipient in the preparation of drug carriers for oral, ophthalmic and nasal administration. After exposu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K9/00A61K47/36
CPCC08B37/006A61K47/36A61K9/0053A61K9/0043A61K9/0048
Inventor 邱琳郑鑫萌周舒文陶思曼雒莉徐以洋陈珺王建浩崔朋飞王程
Owner CHANGZHOU UNIV