Gelatin-based biomedical adhesive and preparation method thereof

A gelatin and biological technology, applied in the direction of surgical adhesives, applications, medical science, etc., can solve the problems of limited practical application range, low bonding strength, non-existence, etc., to improve cross-linking density and gel efficiency, solve The application site is limited and the effect of solving poor biocompatibility

Pending Publication Date: 2022-04-05
SHAANXI UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fibrin glue has the advantages of good biocompatibility, fast curing and biodegradability, but has the disadvantages of low bond strength, low hardness, high risk of infection, and needs to be used in conjunction with traditional wound closure methods such as sutures, etc. ; Cyanoacrylate adhesives have the advantages of strong adhesion, fast curing, and instant adhesion, but there are defects such as too hard tissue bonding parts, formaldehyde after curing, and poor biocompatibility
These two commercially available bioadhesives are less than ideal for adhesion in wet conditions in vivo, thus limiting their range of practical applications
In the prior art, dopamine is also used as the main substance to provide strong adhesion. However, dopamine cannot be stored for a long time because it is easily oxidized. At the same time, the neurological effects of dopamine on the nervous system have always been a concern of people. Therefore, the current tissue adhesion based on dopamine Mixture is almost non-existent

Method used

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  • Gelatin-based biomedical adhesive and preparation method thereof
  • Gelatin-based biomedical adhesive and preparation method thereof
  • Gelatin-based biomedical adhesive and preparation method thereof

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preparation example Construction

[0040] The invention provides a gelatin-based biological hydrogel and a preparation method thereof, the preparation method comprising the following steps:

[0041]Step 1, by weight, dissolving gelatin in 280 parts of 0.01-1mol / L, pH=4-6 phosphate solution, stirring and heating to 40-60°C to dissolve, and adding organic amine compound solution. Among them, the molar ratio of the carboxyl group on the gelatin to the organic amine compound is (1:1) ~ (1:100), the ratio of the number of parts of the gelatin to the organic amine compound is 5: (0.4 ~ 36), using concentrated hydrochloric acid to adjust The pH value of the system is 5.0-6.0, and then 1.2-5.8 parts of carboxyl activators are added to initiate the amination reaction. The reaction temperature of the amination reaction is 37-40° C., and the reaction time is 4-8 hours. After the reaction is completed, the unreacted organic amine compounds in the reaction solution are removed by dialysis, and the product is freeze-dried to...

Embodiment 1

[0065] Step 1, by weight, dissolve 5 parts of gelatin in 280 parts of 0.01mol / L, pH=4 sodium dihydrogen phosphate solution, heat and stir at 40°C to dissolve, add 0.4 parts of ethylenediamine solution, and put on the gelatin The molar ratio of carboxyl and ethylenediamine is 1:1; the pH is adjusted to be 5.0, and then 1.2 parts of carboxyl activator are added to initiate amination reaction, wherein the carboxyl activator is 1-(3-dimethyl Aminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide mixed solution, the carboxyl on the gelatin and the carboxyl activator molar ratio is 1:1, amination reaction The temperature was maintained at 37°C. After the reaction time was 4 hours, unreacted ethylenediamine was removed by dialysis, and aminolated gelatin was obtained after freeze-drying;

[0066] Step 2, by weight, dissolve 2 parts of hyaluronic acid in 200 parts of ultrapure water, stir evenly, and add 1.1 parts of an aqueous solution made of oxidizing agent sodium...

Embodiment 2

[0070] Step 1, by weight, dissolve 5 parts of gelatin in 280 parts of 0.03mol / L, pH=5 sodium dihydrogen phosphate solution, heat and stir at 60°C to dissolve, then add 36 parts of ethylenediamine solution, and put on the gelatin The molar ratio of the carboxyl group and ethylenediamine is 1:100; adopt concentrated hydrochloric acid to adjust the pH to be 6.0, then add 5.8 parts of carboxyl activator to initiate amination reaction, wherein the carboxyl activator is 1-(3 -Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysulfosuccinimide, carboxyl on gelatin to carboxyl activator in a molar ratio of 1:5, aminated The reaction temperature was maintained at 40°C. After a reaction time of 8 hours, unreacted ethylenediamine was removed by dialysis, and aminated gelatin was obtained after freeze-drying;

[0071] Step 2: Dissolve 2 parts of hyaluronic acid in 200 parts of ultrapure water by weight, stir evenly, and add 5.7 parts of an aqueous solution made of oxidan...

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Abstract

The invention discloses a gelatin-based biomedical adhesive and a preparation method thereof.The preparation method comprises the following steps that gelatin is dissolved in a phosphate solution, an organic amine compound is added, the pH value of a system is adjusted, then a carboxyl activating agent is added, and aminated gelatin is obtained after the reaction is completed; the preparation method comprises the following steps: dissolving hyaluronic acid in ultrapure water, uniformly stirring, adding an aqueous solution of an oxidizing agent at room temperature under a dark condition, carrying out an oxidation reaction, and adding a terminating agent to terminate the reaction, so as to obtain formylated hyaluronic acid; and mixing the phosphate buffer salt solution in which aminated gelatin is dissolved with the phosphate buffer salt solution in which aldehyde hyaluronic acid is dissolved, adding tannic acid into the mixed solution, and uniformly stirring to obtain the gelatin-based biomedical adhesive. The preparation method is mild in reaction condition and simple to operate. The prepared adhesive has the characteristics of high bonding speed, high bonding strength, good biocompatibility, biodegradability, good anti-inflammatory property, good anti-oxidation property and good antibacterial property.

Description

technical field [0001] The invention belongs to the technical field of biomedical adhesives, and relates to a gelatin-based biomedical adhesive and a preparation method thereof. Background technique [0002] Traditional methods of wound closure employ surgical sutures and mechanical fixation with rivets, but these methods further damage damaged tissue, leading to increased tension, inflammation, and foreign body reactions. The biomedical adhesive can quickly stop bleeding, shorten the operation time, and is easy to operate. Currently commercial bioadhesives are mainly fibrin glue and cyanoacrylate adhesives. Fibrin glue has the advantages of good biocompatibility, fast curing and biodegradability, but has the disadvantages of low bond strength, low hardness, high risk of infection, and needs to be used in conjunction with traditional wound closure methods such as sutures, etc. ; Cyanoacrylate adhesives have the advantages of strong adhesion, fast curing, and instant adhesi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/08A61L24/00
Inventor 王海花王梦曦武竞衡刘璇魏萌
Owner SHAANXI UNIV OF SCI & TECH
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