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Preparation method of rabdosia amethystoides fermentation preparation and application thereof in anti-tumor treatment

A microbial strain, the technology of Bacillus subtilis, which is applied in the field of fermentation process of traditional herbal medicinal tea in the edible strain Bacillus subtilis, can solve problems such as optimizing the fermentation process

Inactive Publication Date: 2021-07-23
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there is no report on the preparation of microbial traditional Chinese medicine fermentation preparations by utilizing the characteristics of Bacillus subtilis that can grow in plant juice, optimizing the fermentation process, and making full use of their different drug properties, metabolites and biological regulation functions

Method used

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  • Preparation method of rabdosia amethystoides fermentation preparation and application thereof in anti-tumor treatment
  • Preparation method of rabdosia amethystoides fermentation preparation and application thereof in anti-tumor treatment
  • Preparation method of rabdosia amethystoides fermentation preparation and application thereof in anti-tumor treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Preparation of Fragrant Tea Vegetable Bacillus subtilis Fermented Oral Liquid

[0033] 1) Pulverize the raw materials of traditional Chinese medicine fragrant tea and vegetables through a 40-mesh sieve to make fragrant tea and vegetable powder, add 10-15 times the weight of deionized water and 0.5-1 times the weight of brown sugar and soak for 2 hours to form the fragrant tea and vegetable raw material liquid.

[0034] 2) Take 4.5g of beef extract, 9g of peptone, 5g of sodium chloride, 1000mL of deionized water, pH 7.2-7.4, sterilize at 121°C for 20 minutes, and prepare the culture medium;

[0035] 3) Inoculate the bacillus subtilis plate into the above-mentioned strain culture medium after resuscitating, and carry out 10-fold gradient expansion culture. The culture conditions are: 4% inoculum size, 2 days of shaking flask culture at 37 ° C, and the thalline content is 1.0×10 8 -1.0×10 9 CFU / mL; Centrifuge the cultivated bacterial liquid at 8000r / min to pre...

Embodiment 2

[0037] Example 2: Study on the inhibition of DSS-induced colorectal inflammation in mice by fermented oral liquid of Bacillus subtilis

[0038] Twenty 6-8 week old C57BL / 6J mice (18-22 g, female) were purchased from the Institute of Model Animals, Nanjing University. All mice were of SPF grade, and the feeding conditions were 23±2°C and 55±5% humidity. Take 12 hours of light / dark alternation to ensure that the mice drink water normally. Mice were used for experiments after adaptive feeding for one week after purchase. The experimental methods followed the relevant regulations of the Jiangsu Provincial Experimental Animal Management Committee. All experimental procedures were approved by the Ethical Standards Committee for Animal Experiments, Nanjing University School of Medicine. The experiment was divided into control group (CON, 6 rats), enteritis group (DSS, 7 rats), and treatment group (BO, 7 rats). Among them, 6 mice were used as the normal control group, and the rema...

Embodiment 3

[0050] Example 3: Antitumor effect of fermented oral liquid of Bacillus subtilis in fragrant tea dish in mouse AOM / DSS colorectal tumor model

[0051] Twenty-five (18-22 g, female) C57BL / 6J mice aged 6-8 weeks were purchased from the Model Animal Research Institute of Nanjing University. All mice were of SPF grade, and the feeding conditions were 23±2°C and 55±5% humidity. Take 12 hours of light / dark alternation to ensure that the mice drink water normally. Mice were used for experiments after adaptive feeding for one week after purchase. The experimental methods followed the relevant regulations of the Jiangsu Provincial Experimental Animal Management Committee. All experimental procedures were approved by the Ethical Standards Committee for Animal Experiments of Nanjing University. The experiment was divided into control group (5 rats), CAC group (10 rats), and BO treatment group (10 rats). Among them, 5 mice were used as the normal control group, and the remaining mice ...

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Abstract

The invention belongs to the technical field of microbial fermentation of traditional Chinese medicines, and discloses a preparation method of a rabdosia amethystoides preparation fermented by edible fungi and an active effect of the rabdosia amethystoides preparation in resisting digestive tract tumors. The method is characterized in that according to the physicochemical properties of the rabdosia amethystoides, bacillus subtilis (preserved in the China General Microbiological Culture Collection Center with the CGMCC number of 1.15792) is determined as a zymophyte main body by screening food strains, the rabdosia amethystoides is fermented according to a dosage ratio and an optimal culture condition, and the efficient preparation method of the rabdosia amethystoides fermentation preparation is provided. Experiments prove that the bacillus subtilis can effectively degrade crude fiber components of a rabdosia amethystoides plant, active substances of the rabdosia amethystoides plant is fully released, and prepare a rabdosia amethystoides bacteria agent oral liquid. In a mouse AOM / DSS induced colorectal cancer model, the result shows that the oral liquid can inhibit tumor generation and development of a model mouse, enhance the anti-tumor immune effect of the body, prolong the life cycle of the model mouse and improve intestinal flora disorder of the model mouse, and has important application value in digestive tract tumor immunotherapy.

Description

technical field [0001] The invention belongs to the field of microbial fermentation of traditional Chinese medicine, and specifically relates to the fermentation process of traditional herbal medicinal tea in the edible strain Bacillus subtilis, and uses the fermentation process to obtain a preparation that can be taken directly and its improvement and resistance to side effects in the digestive tract of radiotherapy and chemotherapy. Applications in tumor immunity. Background technique [0002] There are more than 152 species in the whole world, and more than 90 species in my country, which are extremely rich in resources. Current studies believe that such plants and their active ingredients have antibacterial, anti-tumor, liver protection, anti-hypoxia, lower blood pressure, and anti-lipid peroxidation damage in vivo and in vitro, and are widely used in traditional Chinese medicine in my country (such as Hu Qingyutang's Wei Fuchun, etc.), but the extraction and identificat...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K36/53A61K9/08A61P35/00C12R1/125
CPCC12N1/20A61K36/53A61K9/08A61P35/00
Inventor 王婷婷赵树立王晓刚沈韬吴思雯
Owner NANJING UNIV
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