61 results about "Digestive Tumor" patented technology

The invention discloses capsules for treating respiratory tract and digestive tract cancers. The capsules are prepared by taking the following traditional Chinese medicines in parts by weight as active pharmaceutical ingredients: 40-60 parts of ginseng, 8-14 parts of calculus bovis factitious, 40-60 parts of pseudo-ginsheng, 10-15 parts of hairyvein agrimonia herb and bud, 8-10 parts of solanum lyratum, 25-45 parts of Chinese Paris rhizome, 25-45 parts of membranous milkvetch root, 25-45 parts of radix sophorae flavescentis, 8-15 parts of ganoderma, 10-30 parts of dried mushroom, 10-30 parts of glabrous greenbrier rhizome, 40-75 parts of longhairy antenoron herb, 8-12 parts of common dysosmatis rhizome and root, 25-45 parts of barbed skullcap herb, 15-21 parts of salvia chinensis, 18-25 parts of spreading hedyotis herb, 10-15 parts of baical skullcap root, 8-12 parts of licorice, 6-14 parts of Indian Iphigenia bulb, 35-55 parts of indigo, 10-15 parts of black nightshade herb, 10-15 parts of asparagus and 8-10 parts of paniculate swallowwort root. The medicament disclosed by the invention is finely extracted from pure Chinese herbal medicines and has obvious analgesic, calmative and anti-cancer effects; the effect of suppressing respiratory tract and digestive tract tumors can achieve 45%; and the medicament is low in price and is a safe and effective treatment way.

The invention belongs to the field of biological pharmacy and provides a monoclonal antibody for resisting cell surface ectopic expression. The amino acid sequence of a light chain variable region at the Fab fragment antigen-binding site of an antibody molecule is shown as SEQ ID NO:2, and the amino acid sequence of a heavy chain variable region is shown as SEQ ID NO:4. The invention also provides application of the monoclonal antibody in preparation of medicaments for treating and preventing digestive tract tumors or digestive tract tumor metastasis. The invention also provides a detection kit and medicinal composition containing the monoclonal antibody. The invention also provides a preparation method of the monoclonal antibody. A series of in vivo and in vitro tests prove that the purified and degermed monoclonal antibody has high affinity for PALP and/or IALP antigen for resisting extracellular ectopic expression of tumor cells and has a biological activating function of inhibiting growth and migration of tumor cells.

The invention provides a digestive tract tumor PDX model and a construction method of a standardized model library. The method comprises the following steps: conducting sampling, treating a collected peripheral blood sample of a digestive tract tumor, obtaining tumor cells, conducting treating and culturing, stably passing the tumor cells to n generations, constructing a digestive tract tumor PDX model and evaluating the model. A Chinese population human-derived digestive tract tumor transplantation animal model and a corresponding primary tumor cell line are established in a digestive tract tumor PDX standardized model library, an important in-vivo model is provided for biological research of tumors, searching of diagnostic markers and drug screening, randomness and blindness of drug use are avoided, disturbance by factors such as original tissue samples is avoided, the digestive tract tumor PDX model can be stably subcultured to three generations or more, and the construction of the digestive tract tumor PDX model standard sample library provides bioinformatics for exploring new targets and searching and analyzing clinical characteristics of digestive tract tumors, and assists in research of a fatal mechanism of digestive tract tumors and treatment of patient tumors.

The present invention discloses one kind of soft lentinan capsule and its preparation process, and the soft lentinan capsule is prepared through suspending lentinan in diluent to form stable suspension and filling the suspension into soft capsule shell. Of each 1000 capsules in 334.45-824.07 g, there are lentinan 10-50 g, diluent 100-400 g, gelatin 50-180 g and glycerin 14.1-64.5 g. The inclusion contains lentinan, diluent and suspending agent; and the capsule shell contains gelatin, glycerin, water, preservative and antioxidant. The soft lentinan capsule has the functions of benefiting Qi, invigorating spleen, tonifying deficiency and strengthening body's resistance, and may be used in treating chronic hepatitis B and as the assisting medicine for radiotherapy and chemotherapy treating tumor in digestive tract. The present invention has the advantages of stable quality, convenient taking and high bioavailability.

The invention provides an anti-CEA (carcinoembrynio antigen) antibody which comprises a heavy chain variable region and a light chain variable region, wherein the complementary determining region of the heavy chain variable region comprises CDR (complementary determining region)-H1 of which the amino acid sequences are shown in SEQ ID NO.1 in the description, CDR-H2 of which the amino acid sequences are shown in SEQ ID NO.2 in the description and CDR-H3 of which the amino acid sequences are shown in SEQ ID NO.3 in the description; and the complementary determining region of the light chain variable region comprises CDR-L1 of which the amino acid sequences are shown in SEQ ID NO.4 in the description, CDR-L2 of which the amino acid sequences are shown in SEQ ID NO.5 in the description and CDR-L3 of which the amino acid sequences are shown in SEQ ID NO.6 in the description. The inventor of the invention screens CEA scFv by using a phage display technology, and thus obtains a high-affinityanti-CEA single-chain antibody. In addition, an anti-CEA chimeric antigen receptor gene is introduced into T cells by using a genetic engineering method to prepare CEA CAR-T, so that CEA CAR-T cellsare capable of specifically recognizing and killing digestive tract tumor cells expressing CEA, and thus the anti-tumor effects of the CEA CAR-T cells can be achieved.

The invention discloses an endoscopic minimally invasive hoe scaler, comprising a catheter, hemostatic forceps, an operating grip and an operation control device. The endoscopic minimally invasive hoe scaler is characterized in that a head of the catheter is connected with the hemostatic forceps while a tail of the catheter is matched connection with the operating grip; the hemostatic forceps are composed of a subwarhead-shaped hollow binding clip of which two halves are closed and opened correspondingly; the hoe scaler is a plasma hoe scaler formed by a steel wire structure, and extends into a linked tube of the hemostatic forceps; the operating grip is provided with a hemostatic forceps push ring and a hoe scaler control grip capable of moving back and forth; and the operation control device comprises a hemostatic forceps operation control device and a hoe scaler operation control device. The endoscopic minimally invasive hoe scaler solves the defects in the prior treatment on digestive tumor, and comprises both the hoe scaler and the hemostatic forceps, so that cockamamie operations of repeatedly changing a plurality of prior instruments in the world at present are avoided; and after being powered on, the endoscopic minimally invasive hoe scaler has the functions of the biopsy forceps and the hemostatic forceps, and the function of incision, so that the detection, the incision and the haemostasis in time can be convenient, and the complications of bleeding can be reduced.

The invention belongs to the technical field of molecular biology and in particular relates to a tumor blood vessel and M1 type macrophage targeting peptide and application thereof. Aiming at the problem that a small molecular targeting peptide for preparing a medicine for diagnosing or treating tumors is lacked, the invention provides the tumor blood vessel and M1 type macrophage targeting peptide TCP-2 and a carrier thereof. The small molecular targeting peptide has an amino acid sequence shown as SEQ ID NO: 1, can be used for specifically targeting tumor blood vessels and M1 type macrophages and can be used for preparing the targeting medicine for diagnosing or treating the tumors; especially, the mall molecular targeting peptide is used for treating digestive tract tumors and providesa new choice for targeting therapy of the tumors.

According to the present invention, TMEM176A gene is firstly adopted as a target gene, and the promoter region in esophageal cancer cells and colon cancer cells presents the high methylation state through a MSP technology; primers and a kit for detecting cell TMEM176A gene promoter region methylation state are provided, wherein the primers are a pair of methylation primers, or a pair of methylation primers and a pair of non-methylation primers; with the primers and the kit, the esophageal cancer detection specificity is good, and the sensitivity is high and achieves 0.5%, wherein the esophageal cancer detection specificity is 61.2%, the colorectal cancer detection specificity is 53.13%, and five cancer cells in 1000 cells can be detected; and the application of the kit to detect the TMEM176A gene promoter region DNA methylation state can be adopted as the powerful tool for digestive tumor diagnosis, treatment effect observation, prognosis determination, minimal residual disease detection and the like, and the advantages of easy operation, good stability, far-reaching clinical significance and promotion are provided.

The inventor finds out a cell population (named as gastric cancer very early cells) which starts to appear in the low-grade heteroplasmal hyperplasia stage of the stomach, has specific molecular characteristics and has a high canceration risk, and the cell population can be used as a marker for very early diagnosis of gastric cancer. Molecular markers of gastric cancer extremely-early cells are applied to preparation of a kit for realizing early diagnosis of gastric cancer or other digestive system tumors based on gastric tissues or blood samples. The gastric cancer extremely-early cell markercan also distinguish the postoperative recurrence risk of gastric cancer and the tumor risk of digestive system organs such as intestines, pancreas and esophagus. Besides, the inventor also finds outa cell population which appears in the early stage of gastric precancerous lesions and has specific molecular characteristics, and the cell population can be used as a marker for early diagnosis of gastric precancerous lesions and is also integrated into the kit. The markers and kits can be used for early clinical diagnosis of gastric cancer and other digestive tract tumors, can also be used as an intervention target for prevention and treatment of the digestive tract tumors, and have a good application prospect.

The invention provides an anti-CEA antibody. The anti-CEA antibody comprises a heavy chain variable region and a light chain variable region, wherein a complementary determining region of the heavy chain variable region comprises CDR-H1 with an amino acid sequence shown as SEQ ID No. 1, CDR-H2 with an amino acid sequence shown as SEQ ID No. 2 and CDR-H3 with an amino acid sequence shown as SEQ IDNo. 3; a complementation determining region of the light chain variable region comprises CDR-L1 with an amino acid sequence shown as SEQ ID No. 4, CDR-L2 with an amino acid sequence shown as SEQ ID No. 5 and CDR-L3 with an amino acid sequence shown as SEQ ID No. 6. The CEA scFv is screened by an inventor of the invention by utilizing a phage display technology, so that the CEA-resistant single-chain antibody with high affinity is obtained. An anti-CEA chimeric antigen receptor gene is introduced into T cells through a genetic engineering method to prepare CEA CAR-T, so that the CEA CAR-T cellsspecifically recognize digestive tract tumor cells expressing CEA and kill the digestive tract tumor cells, and the anti-tumor effect of the CEA CAR-T cells is achieved.

The invention provides a CEA resisting antibody. The CEA resisting antibody comprises a heavy chain variable region and a light chain variable region, wherein the complementary determining region of the heavy chain variable region comprises CDR-H1 of which the amino acid sequence is shown as SEQID No.1, CDR-H2 of which the amino acid sequence is shown as SEQID No.2 and CDR-H3 of which the amino acid sequence is shown as SEQID No.3, and the complementary determining region of the light chain variable region comprises CDR-L1 of which the amino acid sequence is shown as SEQID No.4, CDR-L2 of which the amino acid sequence is shown as SEQID No.5 and CDR-L3 of which the amino acid sequence is shown as SEQID No.6. The inventor uses a phage display technique to screen CEA scFv, so as to obtain a high-affinity CEA resisting single-chain antibody. The chimeric antigen receptor gene for resisting CEA is transmitted into T cells by a genetic engineering method to prepare CEA CAR-T, so that the digestive tract tumor cells of CEA can be specially recognized and expressed by CEA CAR-T cells, and are killed, and the effect of resisting tumors can be realized.

The invention discloses a method for detecting imatinib metabolite in plasma of GIST patients based on non-targeted metabonomics, which comprises the following steps: collecting peripheral venous blood of a plurality of GIST patients who do not take IM or take IM regularly for a long time, processing the peripheral venous blood, performing non-targeted detection on a sample to be detected by adopting a UPLC-QTOF/MS (Ultra Performance Liquid Chromatography-Quadrature Time of Flight/Mass Spectrometry) combined technology to obtain original data of the metabolite in the plasma, performing unsupervised principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA), Student's t-test and difference multiple analysis (FoldChange) on original data, searching differential metabolites, and searching a database to qualitatively determine the GIST metabolic marker. Technical support is provided for effective treatment of GIST patients suitable for IM treatment, and a foundation can be laid for effectiveness, pertinence, noninvasive screening and marker characterization of drug treatment after confirmation of digestive tract tumors.

The invention relates to the technical field of medical treatment, in particular to a sampler special for biopsy of the digestive tract tumor. The sampler comprises a catheter, a sampling box, a sampling mechanism and a pair of clamping cutters. The sampling box is arranged on the head of the catheter through two rotation shafts, a sampling port is formed in the cylinder face of the head of the catheter, and the head of the sampling box fits the sampling port of the catheter; the two rotation shafts penetrate through the sampling box and are inserted in the two ends of the sampling port in thelength direction, a pair of clamping cutters are symmetrically arranged on the head of the sampling box, the tail of each clamping cutter can rotatably sleeve the rotation shaft at the correspondingposition, and the sampling mechanism is arranged in the sampling box and the catheter. The sampler is simple in structure and convenient to operate, all the structures are located in the catheter or fit the outer wall of the catheter, the digestive tract cannot be injured, and during sampling, the clamping cutters clamp and cut the sampling tissue, so that the sampling tissue does not easily falldown and is not easily lost.

The invention relates to the field of medical instruments, and particularly discloses an abdominal cavity drainage fixing device for digestive tract tumor internal medicine, which comprises a first fixing plate, a second fixing plate and a tightening belt, wherein a first semicircular groove opening is formed in the middle on one side of the first fixing plate, one end of the tightening belt is connected to the other side of the first fixing plate, hooks are fixedly connected to the other end of the tightening belt, hanging rings are formed at one end, close to the hooks, of the tightening belt at intervals along the length direction of the tightening belt, a second semicircular groove opening is formed in the middle on one side of the second fixing plate, and a rectangular limiting framewith a hollow square shape is fixedly arranged on the other side of the second fixing plate. The first fixing plate and the second fixing plate are butted with each other, a drainage tube passes through a circular opening formed by the first semicircular groove opening and the second semicircular groove opening and is wound around two vertical limiting columns in an S shape, and a limiting and pressing device presses and fixes the drainage tube to prevent the drainage tube from shaking, and thus the drainage efficiency is ensured, and the pain of patients is reduced, so that the device is suitable for popularization and use.

The invention belongs to the technical field of microbial fermentation of traditional Chinese medicines, and discloses a preparation method of a rabdosia amethystoides preparation fermented by edible fungi and an active effect of the rabdosia amethystoides preparation in resisting digestive tract tumors. The method is characterized in that according to the physicochemical properties of the rabdosia amethystoides, bacillus subtilis (preserved in the China General Microbiological Culture Collection Center with the CGMCC number of 1.15792) is determined as a zymophyte main body by screening food strains, the rabdosia amethystoides is fermented according to a dosage ratio and an optimal culture condition, and the efficient preparation method of the rabdosia amethystoides fermentation preparation is provided. Experiments prove that the bacillus subtilis can effectively degrade crude fiber components of a rabdosia amethystoides plant, active substances of the rabdosia amethystoides plant is fully released, and prepare a rabdosia amethystoides bacteria agent oral liquid. In a mouse AOM/DSS induced colorectal cancer model, the result shows that the oral liquid can inhibit tumor generation and development of a model mouse, enhance the anti-tumor immune effect of the body, prolong the life cycle of the model mouse and improve intestinal flora disorder of the model mouse, and has important application value in digestive tract tumor immunotherapy.

The invention discloses a primer probe group, a kit, a detection method and application for alimentary canal tumor marker detection, and belongs to the technical field of biological medicines. A disclosed marker is a methylated KCNQ5 gene and has at least one modified CpG locus, and the detection method comprises the following steps: extracting DNA in a human-derived biological sample; convertingthe extracted DNA through methylated bisulfite, and converting the unmethylated cytosine C into uracil U; carrying out methylation real-time fluorescent quantitative PCR reaction by adopting the kit;and interpreting according to the Ct value of the KCNQ5 gene, and judging whether the subject suffers from digestive tract tumor or not. The kit is used for detecting specific DNA methylation markersin biological samples of high-risk groups and patients with malignant tumors of digestive tracts, and a high-sensitivity and non-invasive detection means can be provided for early screening, early diagnosis and personalized treatment of malignant tumors of digestive tracts.

The invention provides a biopsy sampler for a digestive tract tumor, belonging to the technical field of medical instruments. The biopsy sampler for the digestive tract tumor comprises a placing plate, wherein the upper surface of the placing plate is provided with a display device and an inflation device, the lower surface of the placing plate is fixedly connected with an outer tube, the outer tube is internally provided with a connecting steel wire and a ventilation tube, the ventilation tube is internally provided with a cable conductor, one end of the connecting steel wire is fixedly connected with a pull ring, and the other end of the connecting steel wire is fixedly connected with a connecting base. Under the action of an inner support frame, an ellipsoidal air bag is greatly deformed at an elastic bag, so a sampling blade drives the fine hair of insoluble dietary fiber to move out of a sampling groove so as to clean dirt on the surface of the tumor, and then a soluble dietary fiber adhesion layer is dissolved, so the fine hair of the insoluble dietary fiber is separated and enters a digestive tract; after the ellipsoidal air bag is deflated, the elastic bag retracts, the sampling blade cuts the tumor and brings the tumor into the elastic bag, and then the tumor can be taken out after inflation.

The embodiment of the invention discloses a digestive tract tumor infiltration layer identification method and device, computer equipment and a medium. The method comprises the following steps: inputting a received ultrasonic endoscope image into a pre-trained first neural network model to obtain an effective area of the ultrasonic endoscope image; inputting the effective area into a pre-trained second neural network model to respectively obtain a mucosa layer area, a mucosa muscle layer area, a submucosa layer area, an innate muscle layer area and a serosa layer area of the digestive tract wall in the ultrasonic endoscope image; sequentially generating minimum circumscribed rectangles of the mucosa layer area, the mucosa muscle layer area, the submucosa layer area, the innate muscle layer area and the serosa layer area; and identifying the alimentary canal tumor infiltration layer area in the ultrasonic endoscope image according to each minimum circumscribed rectangle. According to the method, the digestive tract tumor infiltration layer area of the ultrasonic endoscope image is accurately identified by adopting an artificial intelligence technology, and the accuracy of identifying the digestive tract tumor infiltration layer is improved.

The invention discloses a hericium erinaceus solid beverage. The hericium erinaceus solid beverage is prepared from the following raw materials in parts by weight: 4-5 parts of black hericium erinaceus, 2-3 parts of tremella fuciformis, 15-20 parts of stevioside, 2-3 parts of thiamine hydrochloride, 2-3 parts of riboflavin, 2-3 parts of pyridoxine hydrochloride and the balance of water. Accordingto the invention, the particle size is ensured, the functional components can be fully absorbed by a human body, a health care effect is obtained, hericium erinaceus is adopted as a main raw material,and every 100 g of dry hericium erinaceus contains 26.3 g of a protein, 4.2 g of fat and 856 mg of phosphorus, carbohydrate, calcium, iron, carotene, vitamin B1, vitamin B2 and other components are further used, protein contains various amino acids, the digestive tract tumor treatment effect is good, and thiamine hydrochloride serves as a nutritional supplement to participate in carbohydrate intermediate metabolism in vivo.

The invention belongs to the technical field of medical instruments, and particularly relates to an abdominal drainage fixing device for digestive tract medical oncology, which comprises a main body, a waistband for limiting the main body is arranged on the outer side of the main body, a support for fixing one end of the main body is arranged on the outer side of the waistband, and a support block for supporting the main body is arranged at the upper end of the support. After the main body is placed at the upper end of the supporting block, the pressing belt is pulled to enable the clamping plate to be connected into the clamping groove in a clamped mode, at the moment, pressure can be formed on the main body through elasticity of the pressing belt to fix the main body, discomfort of the wound portion of a patient during operation can be avoided, and the using effect is improved; after the sliding block is connected into the limiting seat in a sliding mode, the limiting pin is connected into the sliding hole in a clamped mode, the support and the limiting seat can be connected into a whole to be fixed, the structure is simple, the using position of the body can be conveniently adjusted according to the wound surface positions of different patients, and meanwhile impurities can be prevented from being left on the outer side of the skin of the patient.