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Use of escitalopram in promoting neural stem cells to express brain-derived neurotrophic factor (BDNF)

A technology of neural stem cells, function, applied in the field of medicine

Active Publication Date: 2021-07-23
张君
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, whether ESCs regulate the expression of BDNF in rat hippocampal-derived NSCs has not been reported

Method used

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  • Use of escitalopram in promoting neural stem cells to express brain-derived neurotrophic factor (BDNF)
  • Use of escitalopram in promoting neural stem cells to express brain-derived neurotrophic factor (BDNF)
  • Use of escitalopram in promoting neural stem cells to express brain-derived neurotrophic factor (BDNF)

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Embodiment Construction

[0022] The present invention will be further described below in conjunction with accompanying drawing and specific embodiment:

[0023] Materials and Methods:

[0024] 1. Isolation and culture of neural stem cells

[0025] Under sterile conditions, take out the hippocampus on both sides of SD rats born within 24 hours, and place them in pre-cooled DMEM / F12 medium; peel off the blood vessels and meninges under a dissecting microscope, and put the isolated hippocampus tissue into another pre-cooled In DMEM / F12 medium, slowly and gently pipette 15-20 times to form a single cell suspension; place in a centrifuge, centrifuge at 1000rpm / min for 5min, discard the supernatant, add serum-free neural stem cell medium to resuspend; count will be 3×10 6 Order of magnitude cells seeded at 25cm 2 culture flask, then placed at 37°C, with a volume fraction of 5% CO 2 cultured in an incubator. After 3 days of culture, half volume of liquid was added, and after 5-7 days, the neurospheres w...

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Abstract

The invention belongs to the technical field of medicines and particularly relates to use of escitalopram in promoting neural stem cells to express brain-derived neurotrophic factor (BDNF). Particularly, 20 ng / ml of escitalopram is added to intervene and induce hippocampus-derived neural stem cells for 7 days. The escitalopram is pioneeringly used for promoting neural stem cells to express BDNF.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of escitalopram in promoting the expression of BDNF by neural stem cells. Background technique [0002] Brain-derived neurotrophic factor (BDNF) is a neurotrophic factor that is mainly synthesized in neurons in the hippocampus, amygdala, midbrain, striatum, and substantia nigra. Amino acid kinase receptor B acts on target tissues, and these parts are closely related to neurocognitive function, long-term enhancement, learning and memory; they play an important role in the growth, development, survival and differentiation of neurons in the nervous system effect. In depression and stress-related affective disorders, the expression of hippocampal BDNF and the concentration of BDNF in plasma are impaired. Although the underlying mechanism of hippocampal damage has not yet been elucidated, many studies have shown that BDNF and its conduction pathways play ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0797
CPCC12N5/0623C12N2501/825
Inventor 顾平张君仇福成刘义晗张永志
Owner 张君