Acid soil microbial remediation agent and preparation method thereof

A technology for microbial remediation and acidic soil, which is applied in the field of acidic soil microbial remediation bacterial agent and its preparation, which can solve the problems of unfavorable plant growth and soil fertility decline, and achieve the effects of improving decomposition efficiency, reducing water, and reducing cracking

Active Publication Date: 2021-07-27
SHANDONG WODIFENG BIOLOGICAL FERTILIZER
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The inventor believes that the acidification process of the soil is accompanied by the lo

Method used

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  • Acid soil microbial remediation agent and preparation method thereof
  • Acid soil microbial remediation agent and preparation method thereof
  • Acid soil microbial remediation agent and preparation method thereof

Examples

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[0049] Preparation Example 1

[0050] S1. Activation of strains: In the ultra-clean workbench, use the inoculation loop to inoculate the jelly-like Bacillus strains and Bacillus licheniformis strains on the nutrient agar medium by streaking inoculation, and cultivate a single colony.

[0051] The preparation of the nutrient agar medium used includes the following steps. After mixing 10g of peptone, 10g of glucose, 3g of beef extract, 3g of yeast extract, 20g of agar and 1000mL of pure water, the temperature is 121°C and the pressure is 0.1Mpa. In a dish, cool and solidify. Blank culture in 34°C constant temperature incubator for 24 hours, if no bacteria grow, it can be used;

[0052] S2. Shake flask inoculation: Add nutrient solution into the shake flask, inoculate the activated Bacillus jelly-like colony and Bacillus licheniformis colony into the nutrient solution, put it in a constant temperature shaker at 34°C, and cultivate it at 180r / min for 12h.

[0053] The preparatio...

Example Embodiment

[0059] Preparation example 2

[0060] The difference from Preparation Example 1 is that only jelly-like bacillus is cultured, and the finally obtained bacteria powder is jelly-like bacillus bacteria powder.

Example Embodiment

[0061] Preparation example 3

[0062] The difference from Preparation Example 1 is that only Bacillus licheniformis is cultured, and the finally obtained bacteria powder is Bacillus licheniformis bacteria powder.

[0063] Preparation of granular decomposed clinker

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Abstract

The invention relates to the technical field of soil remediation, and particularly discloses an acid soil microbial remediation agent and a preparation method thereof. The microbial remediation agent for acid soil comprises 1-3 parts of mixed bacterial powder and 870-990 parts of granular composted materials, and the mixed bacterial powder is prepared from mixed bacterial liquid of bacillus mucilaginosus and bacillus licheniformis. The preparation method comprises the following steps: carrying out primary hot air drying on the granular composted materials, cooling to room temperature, screening, carrying out secondary hot air drying on the granular composted materials meeting the mesh number requirement, cooling to room temperature, carrying out secondary screening, and mixing granular powder meeting the mesh number requirement with the mixed bacterial powder. The acidic soil microbial remediation agent can be used for acidic soil remediation, and has the advantage that soil is not easy to acidify again; in addition, the preparation method has the advantages that the preservation time is prolonged, cracking is reduced, and the performance of the acid soil microbial remediation fungicide is improved.

Description

technical field [0001] The application relates to the field of soil remediation technology, more specifically, it relates to an acidic soil microbial remediation bacterial agent and a preparation method thereof. Background technique [0002] The causes of soil acidification are both natural and man-made. [0003] Under natural conditions, because the amount of rainy precipitation greatly exceeds the amount of evaporation, the leaching effect in the soil is very strong, making the base ions in the soil solution easy to move downward with the infiltration water, the soluble salts in the soil are reduced, and the ions in the soil solution The hydrogen ions replace the base ions on the soil cation exchange site, so that the base saturation of the soil decreases, the hydrogen saturation increases, and the soil acidifies. There are two main causes of soil acidification, one is acid precipitation caused by air pollution; the other is the imbalance of the whole soil system caused b...

Claims

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Application Information

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IPC IPC(8): C09K17/40C05G3/80C05G5/12C05F17/20C05F17/50C12N1/20C12N1/02C12R1/10C12R1/07
CPCC09K17/40C05G3/80C05G5/12C05B17/00C05F17/20C05F17/50C12N1/20C12N1/02C05D1/00C05D3/02C05F5/002C05F11/08Y02W30/40
Inventor 孔令强赵崇强孙厚真
Owner SHANDONG WODIFENG BIOLOGICAL FERTILIZER
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