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A kind of acidic soil microbial remediation bacterial agent and preparation method thereof

A technology for microbial remediation and acidic soil, applied in the field of acidic soil microbial remediation bacterial agent and its preparation, can solve the problems of soil fertility decline, unfavorable plant growth, etc., achieve the effects of reducing water, improving decomposition efficiency, and prolonging storage time

Active Publication Date: 2022-04-15
SHANDONG WODIFENG BIOLOGICAL FERTILIZER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The inventor believes that the acidification process of the soil is accompanied by the loss of nutrients, resulting in a decline in soil fertility, which is not conducive to plant growth

Method used

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  • A kind of acidic soil microbial remediation bacterial agent and preparation method thereof
  • A kind of acidic soil microbial remediation bacterial agent and preparation method thereof
  • A kind of acidic soil microbial remediation bacterial agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0050] S1. Activation of strains: In the ultra-clean workbench, use the inoculation loop to inoculate the jelly-like Bacillus strains and Bacillus licheniformis strains on the nutrient agar medium by streaking inoculation, and cultivate a single colony.

[0051] The preparation of the nutrient agar medium used includes the following steps. After mixing 10g of peptone, 10g of glucose, 3g of beef extract, 3g of yeast extract, 20g of agar and 1000mL of pure water, the temperature is 121°C and the pressure is 0.1Mpa. In a dish, cool and solidify. Blank culture in 34°C constant temperature incubator for 24 hours, if no bacteria grow, it can be used;

[0052] S2. Shake flask inoculation: Add nutrient solution into the shake flask, inoculate the activated Bacillus jelly-like colony and Bacillus licheniformis colony into the nutrient solution, put it in a constant temperature shaker at 34°C, and cultivate it at 180r / min for 12h.

[0053] The preparation of the nutrient solution used ...

preparation example 2

[0060] The difference from Preparation Example 1 is that only jelly-like bacillus is cultured, and the finally obtained bacteria powder is jelly-like bacillus bacteria powder.

preparation example 3

[0062] The difference from Preparation Example 1 is that only Bacillus licheniformis is cultured, and the finally obtained bacteria powder is Bacillus licheniformis bacteria powder.

[0063] Preparation of granular decomposed clinker

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Abstract

The application relates to the field of soil remediation technology, and specifically discloses an acidic soil microbial remediation bacterial agent and a preparation method thereof. A microbial restoration agent for acidic soil comprises 1-3 parts of mixed bacteria powder and 870-990 parts of granular decomposed clinker; the mixed bacteria powder is made from the mixed bacteria liquid of bacillus jelly-like bacillus and bacillus licheniformis, and its preparation method is as follows: : Granular decomposed clinker is dried by hot air for the first time and then cooled to room temperature. After sieving, those that meet the mesh size requirements are dried by hot air for the second time and then cooled to room temperature. After the second sieving, the granular materials that meet the mesh size requirements The powder is mixed with the mixed bacteria powder. The acidic soil microbial remediation bacterial agent of the present application can be used for acidic soil remediation, and it has the advantage of making the soil not easy to acidify again; in addition, the preparation method of the present application has the advantages of prolonging the storage time, reducing cracking, and improving the performance of the acidic soil microbial remediation bacterial agent .

Description

technical field [0001] The application relates to the field of soil remediation technology, more specifically, it relates to an acidic soil microbial remediation bacterial agent and a preparation method thereof. Background technique [0002] The causes of soil acidification are both natural and man-made. [0003] Under natural conditions, because the amount of rainy precipitation greatly exceeds the amount of evaporation, the leaching effect in the soil is very strong, making the base ions in the soil solution easy to move downward with the infiltration water, the soluble salts in the soil are reduced, and the ions in the soil solution The hydrogen ions replace the base ions on the soil cation exchange site, so that the base saturation of the soil decreases, the hydrogen saturation increases, and the soil acidifies. There are two main causes of soil acidification, one is acid precipitation caused by air pollution; the other is the imbalance of the whole soil system caused b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/40C05G3/80C05G5/12C05F17/20C05F17/50C12N1/20C12N1/02C12R1/10C12R1/07
CPCC09K17/40C05G3/80C05G5/12C05B17/00C05F17/20C05F17/50C12N1/20C12N1/02C05D1/00C05D3/02C05F5/002C05F11/08Y02W30/40
Inventor 孔令强赵崇强孙厚真
Owner SHANDONG WODIFENG BIOLOGICAL FERTILIZER
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