Fluorescence immunochromatography test strip for quantitative detection of zeranol as well as preparation method and application of fluorescence immunochromatography test strip

A technology of fluorescence immunochromatography and zearalanol, which is applied in the field of immunochromatography detection, can solve the problems of low sensitivity and limited development, and achieve the effects of high detection sensitivity, high accuracy and good specificity

Inactive Publication Date: 2021-07-27
杭州亦美恒科技有限公司
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  • Description
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Problems solved by technology

At present, the commonly used markers of traditional immunochromatography are AuNPs, but the low sensitivity of colloidal gold immunochromatography limits its development.

Method used

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  • Fluorescence immunochromatography test strip for quantitative detection of zeranol as well as preparation method and application of fluorescence immunochromatography test strip
  • Fluorescence immunochromatography test strip for quantitative detection of zeranol as well as preparation method and application of fluorescence immunochromatography test strip
  • Fluorescence immunochromatography test strip for quantitative detection of zeranol as well as preparation method and application of fluorescence immunochromatography test strip

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Embodiment Construction

[0028] 1 Experimental part

[0029]1.1 Main instruments and reagents: calcium acetate (99.0%), strontium acetate (99.0%), erbium acetate (99.0%), ammonium fluoride, oleic acid (90.0%), octadecene (90.0%), ethylene glycol ( 99.8%), bovine serum albumin (BSA), polyvinylpyrrolidone (PVP), zearalenol, α-zearalenol, β-zearalenol and zearalenol were purchased from Sigma-Aldrich; Cyclohexane, absolute ethanol and concentrated hydrochloric acid were purchased from Sinopharm Chemical Reagent Co., Ltd.; goat anti-mouse secondary antibody was purchased from Wuxi Zhongdeboer Co., Ltd.; Tween-20, diimine hydrochloride (EDC) and sucrose were purchased Tianjin Yongda Chemical Reagent Co., Ltd.; glass fiber membrane and nitrocellulose membrane were purchased from Millipore; bottom plate and absorbent pad were purchased from Shanghai Jinbiao Biotechnology Co., Ltd.; zearalanol monoclonal antibody was purchased from Shanghai Maiboxing Bio Technology Co., Ltd.

[0030] 1.2Ca 0.2 Sr 0.8 Er 3...

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Abstract

The invention relates to the field of immunochromatography detection, in particular to a fluorescence immunochromatography test strip for quantitative detection of zeranol as well as a preparation method and application of the fluorescence immunochromatography test strip. The fluorescence immunochromatography test strip for quantitative detection of zeranol comprises a sample pad, a detection probe labeling combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, and the type of a fluorescence immunochromatography technology is a competitive inhibition immunochromatography method. According to the fluorescence immunochromatography test strip, a novel 808 nanometer excitation up-conversion core-shell nanocrystalline material is adopted for preparing a fluorescence marker, the prepared fluorescence marker is good in specificity, and the further prepared fluorescence immunochromatography test strip has the advantages of being easy to operate, high in detection sensitivity and low in cost, the zeranol can be accurately, rapidly and quantitatively detected, and the application prospect is very good.

Description

technical field [0001] The present application relates to the field of immunochromatography detection, in particular to a fluorescent immunochromatography test strip for quantitative detection of zearalanol and its preparation method and application. Background technique [0002] Zearalanol (Zeranol, ZER), whose trade name in my country is "Hua Da Zhuang", is an effective hormonal animal growth promoter, and was widely promoted in my country in the 1980s. As an exogenous hormone, low concentrations of zearalanol can promote protein synthesis in cattle and sheep, but it has an impact on the development of the human reproductive system and changes in blood thyroxine levels. Studies have also shown that zearalanol can stimulate human breast tumor cells in vitro growth and division. When used in livestock breeding, the growth promoter can accumulate in animal liver, kidney and other tissues or be excreted with urine metabolism, and its drug residues can also affect human health...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558G01N33/543G01N33/533G01N33/52
CPCG01N33/52G01N33/533G01N33/54346G01N33/558G01N33/577
Inventor 王伟丽王兆安郭文胜
Owner 杭州亦美恒科技有限公司
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