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Method for breeding multi-resistance, high-combining ability and excellent yellow modified lines by using compound hybridization method

A technology of compound hybridization and combining ability, applied in the direction of botanical equipment and methods, application, plant gene improvement, etc., can solve the problems of susceptibility to stem rot and poor lodging resistance

Active Publication Date: 2021-07-30
SHIJIAZHUANG ACADEMY OF AGRI & FORESTRY SCI
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Problems solved by technology

[0004] However, some shortcomings of the yellow modified line have not been completely overcome, such as poor lodging resistance in the later stage, susceptibility to stem rot and premature senility, which limit its further utilization; for this reason, we propose a breeding method using compound hybridization. The method of multi-resistance, high combining ability and excellent yellow strain

Method used

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  • Method for breeding multi-resistance, high-combining ability and excellent yellow modified lines by using compound hybridization method
  • Method for breeding multi-resistance, high-combining ability and excellent yellow modified lines by using compound hybridization method

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Embodiment 1

[0031] see Figure 1-2 Shown: the present invention is a method for breeding multi-resistance, high-combining excellent yellow-refined lines using a compound hybridization method, and the method for breeding excellent yellow-reformed lines comprises the following steps:

[0032] Step1: Polymerize and hybridize three backbone yellow-modified materials, and obtain intermediate yellow-modified material Z1 after 5 generations of self-bagging; the Step1 includes:

[0033] ①. Collect the materials Chang 7-2, LX9801 and 5237 of the yellow modified line, carry out polymerization and hybridization, and use them as F1 generation seeds;

[0034] ②. Select excellent individual plants from the progeny groups obtained after planting F1 generation seeds, and screen out plants with good agronomic characteristics, low ear position, harmonious sex, large pollen amount, and high combining ability. Bagging selfing is carried out on multiple screened individual plants to obtain F2 generation seed...

Embodiment 2

[0048] Step 1: Carry out polymerization and hybridization of the yellow modified line materials Chang 7-2, LX9801 and 5237 as the basic material, and obtain the inbred line Z1 after 5 generations of continuous selfing; among them, (1), collect the inbred lines Chang 7-2, LX9801 and 5237 were crossed twice and used as the F1 generation seeds; (2) The progeny population obtained after planting the F1 generation seeds was selected for excellent single plants, and the screened out had good agronomic traits, low ear position, harmonious sex and large pollen amount 1. Plants with high combining ability, after removing the inferior and weak, carry out bagging selfing to a plurality of single plants screened, obtain the F2 generation seeds; (3) use the F2 generation seeds as donors, repeat step (2), continue Plant the seeds of the F2 generation in the multiplication step (2), and so on, obtain a relatively stable excellent inbred line Z1 after selfing 5 times; (4) The characteristics o...

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Abstract

The invention discloses a method for breeding multi-resistance, high-combining ability and excellent yellow modified lines by using a compound hybridization method, and relates to a breeding method of a new plant variety. The method provided by the invention comprises the following steps: Step 1: performing convergent hybridization on three parts of main yellow modified lines namely Chang 7-2, LX9801 and 5237 to obtain a base material, and performing five generations of bagging selfing to obtain an intermediate yellow modified corn material; Step 2, hybridizing a selfing line material agricultural line 178 of the PN78599 group with a material 478 of the REID group to obtain F1-generation seeds, and performing six-generation bagging selfing on the F1-generation seeds to obtain a selfing line material containing disease-resistant and lodging-resistant genes; and Step 3: hybridizing the intermediate yellow modified material and the selfing line material containing the PN78599 polyclonal resistance gene to obtain F1-generation hybrid seeds, and then performing seven-generation bagging selfing on the F1-generation hybrid seeds to obtain the hybrid seeds with outstanding disease resistance and extremely strong lodging resistance. According to the method for breeding multi-resistance, high-combining ability and excellent yellow modified lines by using the compound hybridization method, the problems of poor lodging resistance and low yield of the traditional yellow modified corn selfing line are solved.

Description

technical field [0001] The invention relates to a method for breeding new plant varieties, in particular to a method for breeding multi-resistance, high-combining ability and excellent yellow strains by using a compound hybridization method. Background technique [0002] Corn is the largest food crop in my country. The utilization and promotion of its hybrids have played a leading role in stabilizing my country's food security. Corn breeding and germplasm resources excavation and innovation have always been the primary tasks of scientific researchers. In the 1950s At the beginning, corn breeders selected the first-ring line based on the farm species Sipingtou in Tangshan area, and successfully bred an excellent inbred line Tangsipingtou. Continuous multiple generations of self-crossing, successfully bred an excellent inbred line Huangzaosi, which laid the foundation for my country's resistance to density breeding, and bred well-known varieties such as Yedan 4. Later, Huangzaos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 冯健英王绍新许洛李中建陈莉王宝宝
Owner SHIJIAZHUANG ACADEMY OF AGRI & FORESTRY SCI
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