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Cell preparation for removing wrinkles and preparation method thereof

A cell preparation and cell technology, applied in the field of cell preparation, can solve problems such as poor treatment effect and easy aging

Pending Publication Date: 2021-07-30
纳美细胞科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the skin fibroblasts used in this technology are adult cells, which are prone to aging during the culture process, resulting in poor therapeutic effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of the cell preparation for wrinkle removal proposed by the present invention has the following steps:

[0032] S1: Preparation of Stem Cells

[0033] S11: Wash the placenta obtained during delivery with physiological saline containing antibiotics, and cut the tissue from the decidua basal part of the mother-fetal interface;

[0034] S12: Rinse the tissue clipped in S11 with physiological saline containing antibiotics, cut it into pieces, and add 10 mL of D-Hank's solution containing 0.1% trypsin to digest at 37°C for 4 hours;

[0035] S13: Add 3 ml of DMEM medium containing 10% fetal bovine serum to the digestion mixture of S12 to terminate the digestion;

[0036] S14: Centrifuge the digested mixed solution for 8 minutes at a speed of 1200 rpm, discard the supernatant, and resuspend the cells in DMEM medium containing 10% fetal bovine serum, culture in an incubator, and the cells are 80% confluent After subculture, when the number of cells reac...

Embodiment 2

[0046] The preparation method of the cell preparation for wrinkle removal proposed by the present invention has the following steps:

[0047] S1: Preparation of Stem Cells

[0048] S11: Wash the placenta obtained during delivery with physiological saline containing antibiotics, and cut the tissue from the decidua basal part of the mother-fetal interface;

[0049] S12: Rinse the tissue clipped in S11 with physiological saline containing antibiotics, cut it into pieces, and add 10 mL of D-Hank's solution containing 0.1% trypsin to digest at 37°C for 6 hours;

[0050] S13: Add 3 ml of DMEM medium containing 10% fetal bovine serum to the digestion mixture of S12 to terminate the digestion;

[0051] S14: Centrifuge the digested mixed solution for 12 minutes at a speed of 1800 rpm, discard the supernatant, and resuspend the cells in DMEM medium containing 10% fetal bovine serum, and culture them in an incubator until the cells are 80% confluent After subculture, when the number of...

Embodiment 3

[0061] The preparation method of the cell preparation for wrinkle removal proposed by the present invention has the following steps:

[0062] S1: Preparation of Stem Cells

[0063] S11: Wash the placenta obtained during delivery with physiological saline containing antibiotics, and cut the tissue from the decidua basal part of the mother-fetal interface;

[0064] S12: Rinse the tissue clipped in S11 with normal saline containing antibiotics, cut it into pieces, and add 10 mL of D-Hank's solution containing 0.1% trypsin to digest at 37°C for 5 hours;

[0065] S13: Add 3 ml of DMEM medium containing 10% fetal bovine serum to the digestion mixture of S12 to terminate the digestion;

[0066] S14: Centrifuge the digested mixed solution for 10 minutes at a speed of 1500 rpm, discard the supernatant, and resuspend the cells in DMEM medium containing 10% fetal bovine serum, culture in an incubator, and the cells are 80% confluent After subculture, when the number of cells reached 1×...

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Abstract

The invention discloses a cell preparation for removing wrinkles and a preparation method thereof. The specific method comprises the following steps: S1, preparing stem cells; S2, preparing skin fibroblasts; and S3, mixing the stem cells with the skin fibroblasts, and preparing an injection with a phenol-red-free DMEM culture medium for facial wrinkle removal. The stem cell preparation method comprises the following steps: S11, cleaning a placenta obtained during delivery, and shearing decidua basalis part tissues of a maternal-fetal interface; S12, cleanly rinsing the tissue sheared in the step S11 with physiological saline containing antibiotics, then cutting the tissue into pieces, and adding 10mL of D-Hank's liquid with 0.1% of pancreatin concentration for digestion; S13, stopping digestion of the digestion mixed solution in S12; and S14, centrifuging the mixed solution after the digestion is terminated, discarding the supernate, conducting culturing in an incubator, and cryopreserving the cultured stem cells. The stem cells are compatible with skin fibroblasts, can be used for eliminating wrinkles and filling depressions on the epidermis of the human body, and are obvious, natural and lasting in effect and free of serious side effects.

Description

technical field [0001] The invention relates to the technical field of cell preparations, in particular to a cell preparation for wrinkle removal and a preparation method thereof. Background technique [0002] At present, there are many ways to remove wrinkles. The effect of topical cosmetics is not obvious, and medical injections such as hyaluronic acid have a short-term filling effect. In recent years, skin fibroblast injection wrinkle removal technology has achieved great success in the field of aesthetic medicine. The advantage of this technology is that it increases the thickness and density of the dermis, stretches wrinkles, fills up depressions, and restores the elasticity and luster of the skin. The shape is natural and the effect is long-lasting. However, the skin fibroblasts used in this technology are adult cells, which are prone to aging during the culture process, resulting in poor therapeutic effect. Contents of the invention [0003] Based on the technical...

Claims

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Application Information

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IPC IPC(8): A61K35/545A61K9/08A61P17/18A61P17/00C12N5/071C12N5/073A61K35/33
CPCA61K35/545A61K35/33A61K9/08A61K9/0019A61P17/18A61P17/00C12N5/0656C12N5/0606C12N2509/00C12N2501/115C12N2501/11A61K2300/00
Inventor 李怡林
Owner 纳美细胞科技有限公司
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