Establishment and stemness maintenance method of pluripotent stem cells

A pluripotent stem cell and stemness technology, applied in the field of pluripotent stem cells, can solve the problem of lack of in-depth exploration of pluripotent stem cells, and achieve the effect of promoting differentiation and increasing authenticity.

Pending Publication Date: 2021-08-10
杭州憶盛医疗科技有限公司
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the existing defects and provide a method for the establishment and stemness mainte

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Establishment and stemness maintenance method of pluripotent stem cells
  • Establishment and stemness maintenance method of pluripotent stem cells
  • Establishment and stemness maintenance method of pluripotent stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A method for establishing and maintaining stemness of pluripotent stem cells, comprising the following steps,

[0049] Step 1: Select suitable maternal cells;

[0050] Step 2: extracting maternal cells, culturing the cells, and selecting healthy maternal cells;

[0051] Step 3: Process the maternal cells to obtain new pluripotent stem cells;

[0052] Step 4: Cultivate new pluripotent stem cells and screen healthy new pluripotent stem cells:

[0053] Step 5: Use gene modification technology on pluripotent stem cells to introduce target DNA that is easier to label; Step 6: Induce differentiation and inhibit differentiation experiments on the genetically modified new pluripotent stem cells, and set up different control groups for experiments;

[0054] Step 7: Immunostaining is performed on the differentiated cells to determine the expression of modified genes, and the data are recorded.

[0055] In the present invention, preferably, in Step 1, the bone marrow is collect...

Embodiment 2

[0065] A method for establishing and maintaining stemness of pluripotent stem cells, comprising the following steps,

[0066] Step 1: Select suitable maternal cells;

[0067] Step 2: extracting maternal cells, culturing the cells, and selecting healthy maternal cells;

[0068] Step 3: Process the maternal cells to obtain new pluripotent stem cells;

[0069] Step 4: Cultivate new pluripotent stem cells and screen healthy new pluripotent stem cells:

[0070] Step 5: Use gene modification technology on pluripotent stem cells to introduce target DNA that is easier to label; Step 6: Induce differentiation and inhibit differentiation experiments on the genetically modified new pluripotent stem cells, and set up different control groups for experiments;

[0071] Step 7: Immunostaining is performed on the differentiated cells to determine the expression of modified genes, and the data are recorded.

[0072] In the present invention, preferably, in Step 1, the bone marrow is collect...

Embodiment 3

[0082] A method for establishing and maintaining stemness of pluripotent stem cells, comprising the following steps,

[0083] Step 1: Select suitable maternal cells;

[0084] Step 2: extracting maternal cells, culturing the cells, and selecting healthy maternal cells;

[0085] Step 3: Process the maternal cells to obtain new pluripotent stem cells;

[0086] Step 4: Cultivate new pluripotent stem cells and screen healthy new pluripotent stem cells:

[0087] Step 5: Use gene modification technology on pluripotent stem cells to introduce target DNA that is easier to label; Step 6: Induce differentiation and inhibit differentiation experiments on the genetically modified new pluripotent stem cells, and set up different control groups for experiments;

[0088] Step 7: Immunostaining is performed on the differentiated cells to determine the expression of modified genes, and the data are recorded.

[0089] In the present invention, preferably, in Step 1, the bone marrow is collect...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a pluripotent stem cell establishment and stemness maintenance method. The method comprises the following steps: 1, selecting proper maternal cells; 2, extracting maternal cells, culturing the cells, and selecting healthy maternal cells; 3, treating the maternal cells to obtain novel pluripotent stem cells. According to the invention, the bHLH transcription factors with different concentrations are adopted to inhibit the differentiation condition of the pluripotent stem cells, and the bHLH transcription factors with different concentration gradients are set to explore the pluripotent stem cells, so the influence condition of the bHLH transcription factors with specific concentrations on the differentiation of the stem cells can be confirmed; in this way, the pluripotent stem cells can be placed in the bHLH transcription factor culture solution with specific concentration, and the stemness of the pluripotent stem cells can be maintained, so the pluripotent stem cells can be suitable for subsequent biological or medical research on disease mechanisms, model preparation, chromosome engineering, genome stability and the like.

Description

technical field [0001] The invention belongs to the technical field of pluripotent stem cells, and in particular relates to a method for establishing and maintaining stemness of pluripotent stem cells. Background technique [0002] Pluripotent Stem Cells (Pluripotent Stem Cells) are a type of pluripotent cells with self-renewal and self-replication capabilities. Under certain conditions, it can differentiate into a variety of APSC pluripotent cells. Pluripotent stem cells (HSC) have the potential to differentiate into a variety of cell tissues, but they have lost the ability to develop into a complete individual, and their developmental potential is limited. Pluripotent stem cells (Ps) are the hotspot and focus of current stem cell research. It can differentiate into all cells in the body, and then form all tissues and organs of the body. Therefore, the study of pluripotent stem cells not only has important theoretical significance, but also has great application value in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/074C12N5/10C12Q1/02
CPCC12N5/0696G01N33/5005C12N2501/15C12N2501/998
Inventor 彭伟群曹小伍曹得明邵草品雷铭轩
Owner 杭州憶盛医疗科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap