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Polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material and its application

A polyethylene microsphere, micron-nano technology, applied in the field of biomedical engineering, can solve the problems of uncontrollable embryonic stem cell differentiation, teratoma, non-localization, etc., and achieve the effects of good differentiation potential, promotion of proliferation, and controllable shape.

Active Publication Date: 2021-06-11
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(2) Although embryonic stem cells can differentiate into various cell types, this differentiation is "non-localized". At present, it is not possible to control the differentiation of embryonic stem cells into corresponding cells in specific parts. The current practice can easily lead to teratomas ; and the above-mentioned problems do not exist in the application of adult stem cells
However, because titanium oxide is difficult to disperse evenly in the polyethylene body, there is no form of microspheres made of polyethylene and titanium oxide.
[0006] In addition, microspheres are currently mainly used in the fields of drug sustained release, cell scaffolding, and cell injection. There are no reports on the use of microspheres to promote the proliferation of adult stem cells and maintain the stemness of adult stem cells.

Method used

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  • Polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material and its application
  • Polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material and its application
  • Polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material and its application

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preparation example Construction

[0033] In one embodiment of the present invention, the preparation method ( figure 1 ), including the following steps:

[0034] Immerse polyethylene microspheres in titanium tetrachloride-hydrochloric acid solution, soak in a constant temperature water bath at 60-100°C for 4-24 hours with stirring, harvest the microspheres, wash them with deionized water three times, then wash them with absolute ethanol, and dry them. dry to obtain polyethylene microspheres coated with titanium oxide nanostructures ( figure 2 ).

[0035] In the preparation method of the polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material of the present invention, each step complements each other and forms an organic whole. In the invention, micron-sized polyethylene microspheres are reacted in a titanium tetrachloride-hydrochloric acid solution with a specific concentration and ratio, and nanometer-sized titanium oxide can be grown on the surface of the polyethylene m...

Embodiment 1

[0039] Example 1: Preparation of polyethylene-titanium oxide micro-nano hierarchical structure composite microsphere material

[0040] Polyethylene microspheres (80 μm in diameter) were immersed in the titanium tetrachloride-hydrochloric acid solution (the titanium tetrachloride-hydrochloric acid mixed solution was prepared by the following method: 10ml of titanium tetrachloride was added dropwise to a concentration of 0.1 mol / L hydrochloric acid solution (10ml), prepare titanium tetrachloride-hydrochloric acid mixed solution), stir in 80 ℃ constant temperature water bath (stirring speed is 200rpm) react 12h, harvest microsphere, wash three times with deionized water, and then After cleaning with absolute ethanol, drying and drying, the polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material is prepared.

Embodiment 2

[0041]Example 2: Preparation of polyethylene-titanium oxide micro-nano hierarchical structure composite microsphere material

[0042] Polyethylene microspheres (500 μm in diameter) were immersed in titanium tetrachloride-hydrochloric acid solution (the mixed solution of titanium tetrachloride-hydrochloric acid was prepared by the following method: 10ml titanium tetrachloride was added dropwise to a concentration of 0.25 mol / L hydrochloric acid solution (10ml), prepare titanium tetrachloride-hydrochloric acid mixed solution), stir in 60 ℃ constant temperature water bath (stirring speed is 100rpm) react 24h, harvest microsphere, wash three times with deionized water, and then After cleaning with absolute ethanol, drying and drying, the polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material is prepared.

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Abstract

The invention discloses a polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material and its application. The polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material of the present invention is made by immersing polyethylene microspheres in tetrachloride In the titanium-hydrochloric acid mixture, stir and react in a constant temperature water bath at 60-100°C for 4-24h, wash, and dry to obtain the product. The microsphere has a stable structure and uniform size of 100-500 μm, and the titanium oxide nanorod has a length of 10-50 nm. Seeding human adipose-derived mesenchymal stem cells on the microspheres can promote the proliferation of stem cells and maintain the stemness of stem cells.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a polyethylene-titanium oxide micro-nano multi-level structure composite microsphere material and its application in maintaining stem cell stemness. Background technique [0002] Stem cell therapy technology has shown great clinical application prospects in gene therapy, autoimmune diseases, brain and nervous system diseases, and cardiovascular diseases. Among them, the advantages of autologous adult stem cell transplantation are particularly prominent. [0003] Adult stem cells refer to undifferentiated cells present in an already differentiated tissue. Under certain conditions, adult stem cells either produce new stem cells, or differentiate according to a certain program to produce various specific cell types. Compared with embryonic stem cells, adult stem cells have many advantages, for example: (1) Since the major histocompatibility complex (MHC) of each ind...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/06C12N5/0775C08L23/06
Inventor 孙春辉任娜刘宏甘宜梧李宁波路西恒刘佳丽梁娜谢清华
Owner UNIV OF JINAN
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