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A kind of liver stem cell injection and preparation method thereof

A hepatic stem cell and cell technology, which is applied in the field of hepatic stem cell injection and its preparation, can solve the problem of not yet established a safe and reliable human hepatic stem cell line, achieve better therapeutic effect, reduce injury rate and death rate, and prolong the number of passages. Effect

Active Publication Date: 2021-02-26
BEIJING YULONG SHENGSHI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a safe, reliable, fully functional and immortalized human liver stem cell line has not been established so far.

Method used

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  • A kind of liver stem cell injection and preparation method thereof
  • A kind of liver stem cell injection and preparation method thereof
  • A kind of liver stem cell injection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A hepatic stem cell injection for treating diabetes, each ml injection mixture contains 1×10 7 hepatic stem cells and 1×10 3 umbilical cord mesenchymal stem cells; the injection mixture consists of the following mass percentage components: 2% polyethylene glycol, 2% arginine, 3% lysine, 2% methionine, 4% % valine, 0.2% glacial acetic acid, 3% collagen, 2% glycosaminoglycan, 0.3% retinoic acid and 0.8% ethanolamine; the balance is normal saline.

Embodiment 2

[0034] A hepatic stem cell injection for treating diabetes, each ml injection mixture contains 3×10 5 hepatic stem cells and 1×10 4 umbilical cord mesenchymal stem cells; the injection mixture consists of the following mass percentage components: 8% polyethylene glycol, 5% arginine, 7% lysine, 6% methionine, 7% % valine, 0.8% glacial acetic acid, 5% collagen, 5% glycosaminoglycan, 0.3-0.5% retinoic acid and 1.2% ethanolamine; the balance is normal saline.

[0035] Preparation:

[0036] 1) Culture of liver stem cells;

[0037] 2) Culture of umbilical cord mesenchymal stem cells;

[0038] 3) Polyethylene glycol, arginine, lysine, methionine, valine, glacial acetic acid, collagen, glycosaminoglycan, retinoic acid and ethanolamine are dissolved in normal saline to prepare a compound solution, Pre-cool at 4°C for later use;

[0039] 4) Resuspend the hepatic stem cells obtained in step 1) and the umbilical cord mesenchymal stem cells obtained in step 2) in the compound solution...

Embodiment 3

[0041] A hepatic stem cell medium, based on DMEM / F12 medium, comprising the following components at concentrations: 6mg / ml hyaluronic acid, 60ng / ml hepatocyte growth factor, 50ng / ml basic fibroblast growth factor, 10μg / ml ml recombinant human bone morphogenetic protein, 300 μg / ml 2′,3′-dideoxyadenosine-5-triphosphate, 7 mg / ml ferric citrate, 10 mg / ml carboxymethylcellulose sodium and 3 mg / ml L-carnitine .

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PUM

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Abstract

The invention relates to a hepatic stem cell injection and a preparation method thereof. The injection consists of liver stem cells, umbilical cord mesenchymal stem cells, polyethylene glycol, arginine, lysine, methionine, valine, glacial acetic acid, collagen, glycosaminoglycans, retinoic acid and Ethanolamine is composed according to a certain weight ratio. In the liver stem cell injection of the present invention, liver stem cells and umbilical cord mesenchymal stem cells are combined to have a better therapeutic effect on diabetes; the injection formula of the present invention can maintain the activity of liver stem cells for a long time without any side effects The composition is safe and effective, and the cost is low, and it can be directly applied to clinical injection of diabetes. Using the method for culturing hepatic stem cells and its special medium of the present invention can effectively maintain the optimal state of hepatic stem cells, guide cell proliferation, maintain the stemness of hepatic stem cells, and reduce the damage rate of hepatic stem cells in the process of culturing and harvesting and mortality, shortening the growth cycle.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a liver stem cell injection and a preparation method thereof. Background technique [0002] In the early 20th century, some scholars speculated through some experimental phenomena that the mature liver has a strong regenerative capacity, because there is a group of "ancestor" cells that can differentiate into liver cells and bile duct cells. At present, people have formed a broad consensus on the existence of liver stem cells. It is a kind of stem cells with self-renewal ability and multi-directional differentiation potential. It can produce stem cells with the same phenotype and genotype as itself through its own symmetric and asymmetric division. , can also produce cells of different developmental stages and different differentiation directions in liver tissue, such as hepatocytes, bile duct epithelial cells and pancreatic epithelial cells. With the in-depth understanding of its cha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/407A61K35/28A61K9/10A61P3/10C12N5/0735A61K47/42
Inventor 张晓南吴芳春冯传前邵来
Owner BEIJING YULONG SHENGSHI BIOTECH CO LTD
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