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Method for rapidly estimating microbial concentration

A microbial concentration and rapid technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as limitations and low efficiency of unknown microbial sample concentration, so as to enhance learning interest, cultivate innovative thinking and scientific literacy, The effect of enriching classroom content

Inactive Publication Date: 2021-08-10
CHONGQING THREE GORGES UNIV
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Problems solved by technology

[0003] The observation experience of the current students in the experimental process is relatively simple, and the counting of microorganisms by the hemocytometer is relatively inefficient for quickly estimating the concentration of unknown microbial samples, and is limited to the specific experimental procedures and specific procedures in the microbial experiment textbooks. Therefore, a method for quickly estimating the concentration of microorganisms is proposed

Method used

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  • Method for rapidly estimating microbial concentration
  • Method for rapidly estimating microbial concentration
  • Method for rapidly estimating microbial concentration

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Embodiment Construction

[0026] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention.

[0027] refer to Figure 1-5 , a method for rapidly estimating the concentration of microorganisms, comprising the following steps:

[0028] S1. The structure of the blood cell counting board. The blood counting board is a glass slide that can be used for counting. It is divided into three platforms by four grooves, and the middle platform is divided into two halves, as shown figure 1 , each half has grid lines and is divided into nine large squares by the grid lines, and the central large square is the counting area; the counting area contains two specifications: the first is that the grid lines will count It is divided into 25 middle grids, and the middle grid is d...

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Abstract

The invention discloses a method for quickly estimating microorganism concentration, and the method comprises the following steps: under the multiple of 16 * 40, discovering that a circular view field of a common binocular microscope can be approximately equal to a circumcircle of a middle lattice of a blood cell counting plate with the specification of 25 * 16; deriving according to a mathematical formula: when the magnification factor is 16 * 40, observing and counting by using a common glass slide, and then quickly calculating the concentration of an unknown microorganism sample according to an empirical formula (n is the number of microorganisms in an average view under the magnification factor), and quickly calculating the concentration of the microorganism sample to be detected according to the formula. On the basis of the counting principle and the calculation formula of the existing blood cell counting plate on microorganism experiment teaching materials, innovation and expansion are carried out, and the microorganism sample concentration determination process can be quicker and more efficient by applying the innovated principle and formula.

Description

technical field [0001] The invention relates to the technical field of microorganism concentration, in particular to a method for rapidly estimating the concentration of microorganisms. Background technique [0002] The hemocytometer is a common tool for counting microorganisms in the teaching of the "Microbiology" experimental course at the university level. [0003] The observation experience of the current students in the experimental process is relatively simple, and the counting of microorganisms by the hemocytometer is relatively inefficient for quickly estimating the concentration of unknown microbial samples, and is limited to the specific experimental procedures and specific procedures in the microbial experiment textbooks. Therefore, a method for rapidly estimating the concentration of microorganisms is proposed. Contents of the invention [0004] The purpose of the present invention is to solve the shortcomings in the prior art, and propose a method for rapidly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/06
CPCC12Q1/06
Inventor 刘仁华苏文霞袁雪莲关顺林胡欣谢琴陈燕
Owner CHONGQING THREE GORGES UNIV
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