Construction method of brain metastatic cancer animal model and medical application thereof

A construction method and animal model technology, applied in the field of construction of animal models of brain metastases, can solve problems such as insufficient blood supply to the brain, blockage, and inconformity with physiological characteristics

Inactive Publication Date: 2021-08-13
BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, in the carotid artery injection animal model of brain metastases reported in related studies, the blood vessels on the injection side of the tumor cells are ligated, resulting in the blockage of the blood supply into the cranium. Generally, experimental animals such as mice have a strong compens

Method used

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  • Construction method of brain metastatic cancer animal model and medical application thereof
  • Construction method of brain metastatic cancer animal model and medical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Construction of mouse internal carotid artery injection brain metastases model

[0077] 1. Experimental materials

[0078] The mice were BALB / c mice, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., and the tumor cells were 4T1 mouse-derived breast cancer tumor cells, purchased from the Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences.

[0079] 2. Experimental method

[0080] In this example, all surgical operations are performed using aseptic techniques, and the detailed construction process is as follows:

[0081] (1) After fixing the BALB / c mice, remove the mouse neck hair with scissors, and clean the mouse neck skin with iodine and 75% alcohol;

[0082] (2) Make an incision about 1 cm long on the exposed skin of the mouse with a scalpel;

[0083] (3) Expose the right common carotid artery, carotid artery bifurcation, part of the external carotid artery and internal carotid artery of the mouse wit...

Embodiment 2

[0100] Example 2 Verification of the internal carotid artery injection brain metastasis mouse model constructed by the construction method of the present invention

[0101] The present application adopts the construction method described in Example 1 to construct a mouse model of brain metastases injected into the internal carotid artery. After verification, it is found that the success rate of model construction is very high when the construction method described in the present invention is strictly followed. None of the constructed mouse models failed, and the constructed mouse model of brain metastases by internal carotid artery injection was compared with the mouse models of brain metastases constructed by traditional methods, as well as the brain metastases disclosed in relevant literature or reports so far. As far as the mouse model is concerned, it is more in line with the normal physiological characteristics of the human body.

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Abstract

The invention discloses a construction method of a brain metastatic cancer animal model and a medical application of the brain metastatic cancer animal model, and the internal carotid artery injection brain metastasis cancer mouse model better conforming to the normal physiological characteristics of the human body is constructed for the first time, and compared with a brain metastasis cancer animal model reported in the field at present, the brain metastasis cancer animal model constructed by the method better conforms to the normal physiological characteristics of the human body; the invention provides a brand-new brain metastasis animal model of a non-human mammal for the field, and the model can be used for researching pathogenesis of brain metastasis and can be used for screening of new drugs and effectiveness testing.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a method for constructing an animal model, in particular to a method for constructing an animal model of brain metastases and its medical application. Background technique [0002] With the continuous acceleration of industrialization and urbanization, the gradual aggravation of population aging, and many risk factors such as chronic infection, unhealthy lifestyle, and environmental pollution, cancer has become one of the largest public problems affecting human survival around the world today. First, its morbidity and mortality are increasing year by year (Cao Maomao, Chen Wanqing. Interpretation of GLOBOCAN 2020 global cancer statistics [J]. Chinese Journal of Medical Frontiers (Electronic Edition), 2021, 13(03): 63-69.). With the wide application of nuclear magnetic resonance technology in clinical diagnosis, various new chemotherapy and targeted drugs may penetrate the blood-...

Claims

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Application Information

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IPC IPC(8): A61D1/00A61D7/00
CPCA61D1/00A61D7/00
Inventor 管修东赵璟妍贾旺原林皓张猿
Owner BEIJING TIANTAN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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