A kind of self-assembled nano-biocatalyst and its preparation method and application in butanol production
A biocatalyst and self-assembly technology, applied in physical/chemical process catalysts, chemical instruments and methods, biofuels, etc., can solve the problems of low yield and slow butanol fermentation efficiency, and achieve the goal of reducing toxicity and excellent butanol fermentation The effect of low cost of capacity, production and use
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Embodiment 1
[0035] Example 1: Blank group
[0036] (1) Bacterial acquisition of Clostridium beijerinckii: inoculate the preserved Clostridium beijerinckii liquid into P2 medium for activation and culture, and then inoculate it into fresh P2 medium according to the ratio of 1:20, at 140rpm, 37°C Conditioned culture for 60h to obtain Clostridium beijerinckii liquid, and the biomass of the obtained Clostridium beijerinckii liquid is measured, and the test results are as follows figure 1 shown. Depend on figure 1 It can be seen that in the absence of Cd (NO 3 ) 2 In the case of adding, the OD of the Clostridium beijerinckii liquid after culturing for 60h 600 It reached 2.23±0.11, indicating that the growth state of the bacteria was good.
[0037] The transmission electron scanning electron microscope test was carried out on the obtained Clostridium beijerinckii bacteria, and the test results were as follows figure 2 shown. Depend on figure 2 It can be seen that there is no formation...
Embodiment 2
[0039] Example 2: Insert 0.1g / L of Cd(NO 3 ) 2
[0040] (1) Obtainment of self-installed nano-biocatalysts: Inoculate the preserved Clostridium beijerinckii liquid into P2 medium for activation and culture, and then inoculate it into fresh P2 medium according to the ratio of 1:20, and inoculate it into P2 medium at the same time. Add 0.1g / L of Cd(NO 3 ) 2 (The access method is to use anaerobic water to convert Cd(NO) 3 ) 2 ·4H 2O is configured to a concentration of 5 g / L mother liquor, and then the mother liquor is inserted into the P2 medium in proportion), and then cultured for 60 h at 140 rpm and 37 °C.
[0041] The biomass of the obtained Clostridium beijerinckii liquid is measured, and the test results are as follows: figure 1 shown. Depend on figure 1 It can be seen that at 0.1 g / L Cd (NO 3 ) 2 In the case of adding, the OD of the Clostridium beijerinckii liquid after culturing for 60h 600 It reached 2.91±0.02, indicating that the growth state of bacteria was...
Embodiment 3
[0047] Example 3: Insert 0.2g / L of Cd(NO 3 ) 2
[0048] (1) Obtainment of self-installed nano-biocatalysts: Inoculate the preserved Clostridium beijerinckii liquid into P2 medium for activation and culture, and then inoculate it into fresh P2 medium according to the ratio of 1:20, and inoculate it into P2 medium at the same time. Add 0.2g / L of Cd(NO 3 ) 2 , cultured at 140rpm and 37℃ for 60h.
[0049] The biomass of the obtained Clostridium beijerinckii liquid is measured, and the test results are as follows: figure 1 shown. Depend on figure 1 It can be seen that at 0.2g / L Cd(NO 3 ) 2 In the case of adding, the OD of the Clostridium beijerinckii liquid after culturing for 60h 600 It reached 1.12±0.05, indicating that the growth state of the bacteria was inhibited by cadmium ions.
[0050] The obtained Clostridium beijerinckii liquid was tested by transmission electron scanning electron microscope, and the test results were as follows Figure 4 shown. Depend on Fi...
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