A strain of A. japonica, cell suspension of A. japonica and its application in the control of Fusarium wilt of pepper
A cell suspension, Fusarium oxysporum technology, applied in the application, bacteria, fungicides and other directions, can solve the problems of unsatisfactory control effect, unreported biocontrol bacteria, etc., and achieves great application value, remarkable control effect, and easy operation. Effect
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Embodiment 1
[0024] The isolation and identification of embodiment 1 bacterial strain
[0025] 1. Strain isolation:
[0026] The strain isolation samples were collected from the larvae of the onion field fly in the garlic field of Fan Town, Tai'an City, Shandong Province. The collected larvae were put into sealed bags and recorded. Sealed bags were stored in ice boxes and shipped back to the laboratory for later use. After the larvae were brought back to the laboratory, the surface of the larvae was disinfected with 75% ethanol disinfectant for 15 seconds, and then cleaned with sterile water. The disinfection and cleaning process was repeated three times. The sterilized larvae were placed under a dissecting microscope, the head and tail of the larvae were cut off with ophthalmic surgical scissors, and then the larvae were cut open from the side of the worm body to take out the intestinal tract.
[0027] Place the larval gut in a 1.5 mL centrifuge tube, add 100 μL of phosphate buffer, an...
Embodiment 2
[0035] Embodiment 2 The confrontation culture test of the fungus M1031 to Fusarium oxysporum capsicum specialization type
[0036] After using LB solid culture medium to cultivate the Pseudomonas M1031 obtained in Example 1 for 24 hours, transfer it to the LB liquid medium with an inoculation loop and shake it for 8 hours to obtain the Pseudomonas M1031 liquid;
[0037] Inoculate the Fusarium oxysporum capsicum-specialized bacterial cake with a diameter of 4 mm on the center of the PDA plate, and then use the inoculation loop to draw lines on the left and right sides of the Fusarium oxysporum capsicum-specialized bacterial cake, and draw The line position is 15mm away from the Fusarium oxysporum capsicum-specialized bacterial cake; the PDA plate not inoculated with the M1031 liquid was used as a blank control.
[0038] Set up 5 parallel controls for each P. oxysporum M1031, and observe the growth and inhibition zone of Fusarium oxysporum capsicum-specialized type after culturi...
Embodiment 3
[0039] The application of embodiment 3 Pianian bacteria M1031 in capsicum wilt
[0040] Experimental location and pepper varieties: At the beginning of August 2019, a field experiment was carried out in a double-cropping pepper greenhouse at the experimental base of the Caishi Campus of the Shandong Academy of Sciences. The pepper variety is Changfeng 101 Xianjiao.
[0041] Cell suspension preparation:
[0042] (1) On the LB solid plate obtained in Example 1, inoculate the newly activated Phytophthora M1031 in a test tube equipped with 3mL liquid LB, and cultivate overnight at 30°C and 150rpm to obtain a culture;
[0043] (2) Transfer the culture to an Erlenmeyer flask containing 150 mL of liquid LB medium, and culture it at 30°C and 150 rpm for 48 hours to obtain a culture solution; centrifuge the culture solution at 5000 rpm for 10 minutes, discard the supernatant, Use sterile water to resuspend the M1031 cells of P. 10 CFU / mL of cell suspension.
[0044] Test treatment:...
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