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Fusarium equiseti L1293 and application thereof

A technology of Fusarium equiseti, strains, applied in fungi, microorganisms, biochemical equipment and methods, etc., can solve the problems of dependence and high price

Active Publication Date: 2021-08-17
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the standard substances of zearalenone (ZEN) required for domestic testing rely on imports, which are expensive

Method used

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  • Fusarium equiseti L1293 and application thereof
  • Fusarium equiseti L1293 and application thereof
  • Fusarium equiseti L1293 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, isolation and identification of bacterial strain L1293

[0015] The strain was isolated from contaminated corn in Xinzheng City, Henan Province.

[0016] Weigh 10g of corn seeds, soak them in 75% alcohol for 15-20s, then transfer them into 100mL sterile water, shake them for 30min, put them in a 25°C incubator and cultivate them for 4-5d, when mycelium or When there is a mold layer on the surface of the seeds, move the hyphae or mold layer onto the PDA medium and cultivate them at a constant temperature of 25°C. Check the medium every day, isolate and purify the single colony, and obtain 13 pure strains of purified strains.

[0017] Pick 13 pure strains to the center of the PDA medium plate with an inoculation needle, and cultivate them in a 25°C incubator. The 13 pure strains grew well on the PDA medium. The colonies were round in shape, off-white to light yellow, and the aerial hyphae were short and cotton-like. In the later stage, the color of the col...

Embodiment 2

[0020] Embodiment 2, utilize strain L1293 to prepare zearalenone (ZEN)

[0021] (1) Prepare the seed liquid of bacterial strain L1293

[0022] Bacterial strain L1293 was cultured in a PDA plate at 25°C for 3 days, and then the hyphae pieces were picked and inoculated in a 300ml Erlenmeyer flask containing 100ml seed medium (PDB medium), and cultured with shaking at 25°C for 3 days as a seed solution.

[0023] (2) Preparation of bacterial strain L1293 solid fermentation culture

[0024] Insert 5 ml of the seed solution into a 500 ml Erlenmeyer flask (80 g of corn slag / 100 ml of distilled water, obtained by sterilizing at 121°C for 20 minutes) equipped with wheat culture medium, and cultivate it in the dark at 25°C for 3 weeks to obtain a solid fermentation culture of bacterial strain L1293, and ferment Scale 20 bottles.

[0025] (3) Preparation of chloroform-methanol gradient eluent with a volume ratio of 10:1

[0026] The fermentation culture was dried at 55-58°C, weighed (...

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Abstract

The invention belongs to the technical field of preparation of zearalenone through microbial fermentation and discloses a fusarium equiseti L1293 strain with an accession number of CGMCC No. 19032. The fusarium equiseti L1293 strain is preserved in China General Microbiological Culture Collection Center, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China on December 10, 2019. The strain provided by the invention has efficient zearalenone (ZEN) synthesis capability.

Description

technical field [0001] The invention belongs to the technical field of preparation of zearalenone by microbial fermentation. Background technique [0002] Mycotoxins are secondary metabolites secreted by toxin-producing fungi that can cause various damages to humans and animals. Zearalenone (ZEN), also known as F-2 toxin, has reproductive, immune, liver, cell toxicity and carcinogenicity, which seriously threatens the health and safety of animals and humans. As people attach great importance to the health and safety of food, the detection of mycotoxins is becoming more and more stringent, and the detection range is becoming more and more extensive. At present, most of the standard substances of zearalenone (ZEN) required for domestic testing rely on imports, which are expensive. In order to ensure the supply of ZEN standard materials required for detection and analysis, reduce import dependence. Screening and obtaining high-yield strains of ZEN, fermenting and cultivating...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/08C12R1/77
CPCC12N1/14C12P17/08
Inventor 刘宏伟韩俊杰
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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