A microfluidic device for deformable beads enrichment and self-regulated ordering and encapsulation in droplets

Abstract

Disclosed herein are microfluidic devices comprising, one or more inlets in flow communication with one or more microfluidic channels, wherein the one or more inlets are adapted for receiving deformable beads, oil, and / or a suspension comprising buffer, cells, and / or particles, wherein the one or more microfluidic channels are in flow communication with the one or more inlets through a cross junction and define a fluid flow path therebetween, said fluid flow path forming a substantially planar substrate, and wherein the microfluidic channel is adapted to generate droplets. Also disclosed are methods of making and using the same.

Description

[0001] This application claims priority to co-pending U.S. Provisional Application No. 62 / 790,369, filed January 9, 2019, the entire contents of which are incorporated herein by reference. technical field [0002] The field of the invention relates to microfluidic devices in the medical and biotechnology industries, in particular devices for deformable bead enrichment and self-regulating sorting and encapsulation in droplets. Background technique [0003] All publications herein are incorporated by reference as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference. To the extent that a definition or use of a term in an incorporated reference is inconsistent or contrary to the definition of that term provided herein, the definition of that term provided herein applies and not the definition of that term in the reference. [0004] The following description includes information that may be helpful in und...

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Problems solved by technology

However, this sorting design significantly increases the flow resistance in the microchannel and is not suitable for constant pressure source applications

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