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Akkermansia muciniphila strain and use thereof

A mucin and strain technology, applied in the fields of peptide/protein components, bacteria, applications, etc., can solve problems such as increased UCP-1 expression

Pending Publication Date: 2021-08-31
KO BIOLABS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Beige adipocytes are induced from white adipocytes, which are harmful to health, by stimuli such as exercise or cold, and the properties of white adipocytes are reduced, but become characteristic of brown adipocytes, resulting in increased expression of UCP-1

Method used

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  • Akkermansia muciniphila strain and use thereof
  • Akkermansia muciniphila strain and use thereof
  • Akkermansia muciniphila strain and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Analysis of the effect of Example 1 reduced mucin administration addicted Ackerman tarda (Akk) strain to high fat diet mouse model of liver and BAT weight

[0087] Brain heart infusion supplemented with 0.5% mucin (BHI) medium anaerobically cultured addicted solid mucin Ackerman coli (ATCC BAA-835, Akk) strain for 72 hours to give a stock solution and to ensure. Intake of high-fat diet (60% fat) (HF + Akk, n = 8 / group), the daily C57BL / 6 male mice at 6 weeks of age to 4 × 10 8 CFU / 200μl / mouse orally administered a concentration of the strain. Intake of low-fat diet (10% fat) diet (LF) of the high-fat diet group and the diet intake only (HF) is used as a control group. After 14 weeks, compared to a control group and the administration group of strains. After fasted for 16 hours, the collection of adipose tissue and liver tissue, tissue weight was measured (FIG. 1A).

[0088] A result, it was confirmed as compared with HF group, HF + wt inguinal white adipose tissue A...

Embodiment 2

[0091] Example 2. tropic strain mucin Ackerman's increased expression of UCP-1 and BAT associated markers

[0092] In the brown adipose tissue (iBAT), the uncoupling protein as a brown fat activation markers (UCP-1) Immunohistochemistry (IHC) staining, and in each group (Figure 2A). After extraction of the tissue RNA was synthesized cDNA, confirmed that UCP-1 gene expression by qPCR, and also confirmed that the brown adipose differentiation-related markers (CIDEA, PRDM16, PPARGC1α, Ai Palin peptide (of Apelin)) (FIG. 2B).

[0093]As the experimental results, it was confirmed that the brown fat-related markers in the brown adipose tissue in which high-fat induced endobacillus were significantly increased compared to the non-feed group, and was related to brown fatty activity. The dyeing result of the UCP-1 factor confirmed this increase. Therefore, it is confirmed that the mechanism of admiscus induced brown fat is confirmed.

Embodiment 3

[0094] Example 3. Il-6 cytokines and GLP-1 in Albumin, Albinocellularia

[0095] After extracting RNA from the ileum and colon tissue and synthesized CDNA, the expression level of immunocytocytic markers (TNF-α, IL-1β, IL-18, IL-6, IL-10) of each group were compared (Fig. 3A and Figure 3b).

[0096] The use of three types of lactacillus strain (KCTC2180, KCTC3112, KCTC1048), three types of bifidobacterium strain (KCTC3127, KCTC3128, KCTC3352) or endomasis Akmania ( AKK) Treatment of mouse intestinal cell line (CT26 cells), comparing the ability of IL-6 cytokines expression. The lipopolysaccharide (LPS) from E. coli is used as a positive control (Fig. 3c).

[0097] Related genes (GCG, PCSK1, PCSK2) (GCG, PCSK1, PCSK2) (GCG, PCSK1, PCSK2) (Fig. 3D) were identified by QPCR.

[0098] As the experimental results, IL-6 cytokines in the shells and colon cells were confirmed by administration of Il-6 cytokines in mice, and serum appetite regulatory peptide-1 (GLP- 1) Expression significan...

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Abstract

The present invention relates to a Akkermansia muciniphila SNUG-61027 strain (accession number KCTC 13530BP) and a use thereof. Specifically provided are: a composition for appetite control or prevention, improvement, alleviation or treatment of metabolic diseases, containing, as an active ingredient, the strain, a culture liquid thereof and the like, or a B2UM07 protein isolated therefrom; a use of the composition for appetite control or prevention, improvement, alleviation and treatment of metabolic diseases; and a method for appetite control or prevention, improvement, alleviation and treatment of metabolic diseases by using the composition. Therefore, the present invention exhibits effects of weight loss and glucose homeostasis control, from among anti-obesity effects, and influence on brown fat and secretion of an appetite control hormone secretion.

Description

Technical field [0001] Cross-reference [0002] The benefits of the Korean Patent Application No. 10-2018-0125670, filed on October 10, 2019, which are submitted in the Korean Patent Application Serial No. 10-2019-0125670, filed on October 10, 2018, filed on the approved by the Korean Intellectual Property Office. Each of the applications is incorporated herein by reference. [0003] The present invention relates to an effective inhibiting appetite and prevention, alleviating, alleviating, akkermansia muciniphila, Snug-61027 strain (deposit No. KCTC 13530BP) and its use thereof. Background technique [0004] Obesity is due to changes in eating habits (such as high-calorie diet, lack of exercise, etc.), accumulating excessive fat caused by excessive fat, related to type 2 diabetes, cardiovascular disease, liver disease and various cancers, and therefore It has important clinical significance. On the other hand, it is known that intestinal microorganisms are closely related to met...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61K38/16A61P3/04A61P3/00A61P3/10A61P9/10A61P3/06A61P1/16A61P9/00A23L33/135C12R1/01
CPCC12N1/20A61K35/74A61K38/164A61P3/04A61P3/00A61P3/10A61P9/10A61P3/06A61P1/16A61P9/00A23L33/135A23V2002/00A23V2200/30C12N1/205C12R2001/01C12N9/52A61K2035/115A23V2200/332A23L33/40A61P3/08A61K38/482C12Y304/21102
Inventor 高光杓尹窙信赵廷桓柳炫朱南泰旭
Owner KO BIOLABS INC
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