A porous microsphere adhesive with osteoinductive ability and its preparation method
A technology of porous microspheres and adhesives, which is applied in the fields of prosthesis, tissue regeneration, and medical science, and can solve the problems of limited fracture fixation applications, poor adhesion and mechanical properties of medical porous microsphere adhesives, etc. Achieve reliable biological activity, improve efficiency, and increase reaction speed
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Embodiment 1
[0031] A porous microsphere adhesive with osteoinductive ability, the specific preparation method comprises the following steps:
[0032] 1. Weigh 10g of sodium alginate, add 3kg of deionized water, keep warm in the range of 50±5°C and magnetically stir to fully dissolve the solid phase to obtain a solution; continue to weigh 1.5g of diammonium hydrogen phosphate and dissolve in the solution, as an aqueous solution;
[0033] 2. Weigh Span 80, glycerol and secondary recrystallized phosphatidylserine, wherein the mass ratio of each component is 1% for Span 80, 60% for glycerol, and 39% for secondary recrystallized phosphatidylserine, totaling 100% ; First dissolve Span 80 in glycerol at 50±5°C, then add the secondary recrystallized phosphatidylserine to fully dissolve with magnetic stirring, and use it as an oil phase solution. Pour the water-phase solution into the oil-phase solution, mix the volume ratio of the water-phase solution: oil-phase solution=7:2, add bioactive glass...
Embodiment 2
[0037] A porous microsphere adhesive with osteoinductive ability, the specific preparation method comprises the following steps:
[0038] 1. Weigh 25g of sodium alginate, add 3.5kg of deionized water, keep warm in the range of 50±5°C and magnetically stir to fully dissolve the solid phase to obtain a solution; continue to weigh 2.5g of diammonium hydrogen phosphate and dissolve in the solution , as an aqueous solution;
[0039] 2. Weigh Span 80, glycerol and secondary recrystallized phosphatidylserine, wherein the weight ratio of each component is 3% for Span 80, 65% for glycerol, and 32% for secondary recrystallized phosphatidylserine, totaling 100% ; First dissolve Span 80 in glycerol at 50±5°C, then add the secondary recrystallized phosphatidylserine to fully dissolve with magnetic stirring, and use it as an oil phase solution. Pour the water phase solution into the oil phase solution, the mixing volume ratio water phase solution: oil phase solution=8:3, add bioactive glas...
Embodiment 3
[0085] 1. The porous microsphere adhesives prepared in Example 1 and Comparative Example 2 (the difference between the two is whether they contain magnesium) were cured respectively, and the cured samples were extracted at the ratio of 0.2g sample / 1mL extraction medium. The extraction medium is clear MEM medium, 37 ± 1 °C, (24 ± 2) h to prepare the extraction solution, and the test solution is taken according to the extraction solution method specified in GB / T16886.5-2017. Cytotoxicity is shown in the table below:
[0086]
[0087] The experimental results showed that several compositions had no potential cytotoxicity.
[0088] 2. The bonding properties of the porous microsphere adhesives prepared by the methods described in the examples and comparative examples were tested, and sheep bones and metals were used as the bonding objects respectively. Before bonding, soak the bonded section in simulated body fluid for 1 hour to ensure the wet environment of the bonding surface...
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