Composition and feed for feeding coccinella septempunctata, and preparation method of composition
A technology of ladybird seven star and a composition, applied in the field of a composition for feeding ladybird seven star and the field of preparation thereof, can solve the problems of being difficult to maintain enough to control the harm of aphids, high economic cost, limited utilization mode, and the like
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[0036] The preparation method of above-mentioned composition comprises the following steps:
[0037] (1) the aphid treatment is mixed with sucrose, potassium sorbate and purified water to obtain the first solution;
[0038] (2) β-carotene is mixed with purified water to obtain a second solution;
[0039] (3) Mix the first solution, the second solution and the juvenile hormone, add an appropriate amount of pure water to constant volume, and obtain the composition, (the juvenile hormone is a low-concentration and high-efficiency hormone substance, and is insoluble in water. Add after organic solvent (75% alcohol) is dissolved into the solution of higher concentration, because the overall content of juvenile hormone is extremely low, the solvent used can not affect the breeding of ladybug). The mixture is initially made into a paste, and the obtained mixture is filled into a semi-ellipsoid container with a diameter of 0.5-2.0 cm and a height of 0.2-0.5 cm, sealed with a parafilm...
Embodiment 1
[0043] In order to clarify the optimum concentration of β-carotene, the following experiments were designed:
[0044] Based on the basic formula (750g of aphid treatment + 150g of sucrose + 100ml of purified water + 2g of potassium sorbate) as the main body, a total of 5 treatment groups were designed: A, B, C, D and E, A was the basic formula plus 0.5g of β-carotene, B Add 1.0g of β-carotene to the basic formula, add 1.5g of β-carotene to the basic formula of C, add 2.0g of β-carotene to the basic formula of D, and add 2.5g of β-carotene to the basic formula of E. Control experiments of pure feed and pure aphid feeding were carried out at the same time. The test time was 30 days, and the feeding situation, egg laying and hatching situation of each group of ladybugs were observed, and the optimal concentration of β-carotene was screened out.
[0045]The indoor environment is set at a temperature of 25±1°C, a humidity of 75%, and a light of 16h / d. Use a petri dish as a breedi...
Embodiment 2
[0057] In order to clarify the optimum concentration of juvenile hormone, the following experiments were designed:
[0058] Existing studies have shown that: low-concentration juvenile hormone has the characteristics of promoting the development of insect ovaries, but high-concentration juvenile hormone can inhibit the normal growth and development of insects. It can improve the egg-laying efficiency of ladybugs.
[0059] The test took the basic formula (750g of aphid treatment + 150g of sucrose + 100ml of purified water + 2g of potassium sorbate) as the main body, and designed 5 treatment groups: A, B, C, D, E, A was the basic formula plus juvenile hormone 1μL, B Add 5 μL of juvenile hormone to the basic formula, 10 μL of juvenile hormone to the basic formula of C, 20 μL of juvenile hormone to the basic formula of D, and 50 μL of juvenile hormone to the basic formula of E. At the same time, the control experiment of pure feed and pure aphid feeding was carried out . The tes...
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