A method for guiding nanoparticle superlattices using DNA origami and DNA tiles
A nanoparticle and superlattice technology, applied in the biological field, can solve problems such as limiting the adjustment of DNA origami spacing, soft crystals are easily affected by the environment, etc., and achieve the effect of improving rigidity and compression resistance, and improving overall rigidity
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Embodiment 1
[0024] A method of guiding nanoparticle superlattice using DNA origami and DNA tiles, including the following steps:
[0025] (1) Synthesis of DNA origami octahedron: By mixing 10 nm M13 MP18 SCAFFOLD DNA and 144 single-stranded DNA in a buffer containing 1 mM EDTA, 12.5 mM magnesium acetate, and the mixed solution is cooled from 90 ° C to room temperature for more than 20 hours. Slow annealing to fold the DNA origami octahedron frame; the mole rather ratio of the M13 MP18 SCAFFOLD DNA and the single-stranded DNA is 1:10.
[0026] (2) Synthesis of DNA tile structure: By mixing SSDNA in 0.3 m PBS buffer, the mixture was cooled from 95 ° C to room temperature within two days; the DNA tile structure; the PBS buffer The medium contains 0.3 mNAcl, 10 mm phosphate. The DNA tile structure is a double stranded DNA.
[0027] (3) Synthesis of three-dimensional crystal nanoparticle superlattice: DNA origami octahedron to which the DNA origami, an excess SSDNA-functionalized gold nanoparticle...
Embodiment 2
[0029] Embodiment 2 The difference from Embodiment 1 is:
[0030] A method of guiding nanoparticle superlattice using DNA origami and DNA tiles, including the following steps:
[0031] (1) Synthesis of DNA origami octahedron: By mixing 10 nm M13 MP18 SCAFFOLD DNA and 144 single-stranded DNA in a buffer containing 1 mM EDTA, 12.5 mM magnesium acetate, and the mixed solution is cooled from 90 ° C to room temperature for more than 20 hours. Slow annealing to fold the DNA origami octafous frame; the molar ratio of the M13 MP18 SCAFFOLD DNA and the single-stranded DNA is 1:20.
Embodiment 3
[0033] Example 3 The difference from Embodiment 1 is:
[0034] A method of guiding nanoparticle superlattice using DNA origami and DNA tiles, including the following steps:
[0035] (1) Synthesis of DNA origami octahedron: By mixing 10 nm M13 MP18 SCAFFOLD DNA and 144 single-stranded DNA in a buffer containing 1 mM EDTA, 12.5 mM magnesium acetate, and the mixed solution is cooled from 90 ° C to room temperature for more than 20 hours. Slow annealing to fold the DNA origami octahedron frame; the molar ratio of the M13 MP18 SCAFFOLD DNA and the single-stranded DNA is 1: 15.
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