Immune checkpoint inhibitor related myocarditis mouse model and construction method
An immune checkpoint and mouse model technology, applied in the field of medical animal models, to achieve the effects of easy promotion and application, increased success rate, and significant myocarditis manifestations
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[0044] 1. Experimental method:
[0045]1) 30 male BALB / c mice aged 6 weeks were selected and divided into 3 groups, 10 in each group: divided into control group (control), autoimmune myocarditis group (TnI group), ICI-associated myocarditis group (TnI+ anti-PD-1 group); in addition to the control group, mice were subcutaneously injected with 0.1ml of complete Freud containing 0.25mg of mouse cardiac troponin I (TnI) peptide on day 0 and day 7, respectively. Adjuvant. The ICI-associated myocarditis group received intraperitoneal injection of PD-1 inhibitor (InVivoMab anti-mouse PD-1) at a dose of 5 mg / kg / time every 2 days from day 7 for a total of 5 times. The changes of cardiac function and molecular levels in the acute myocarditis inflammatory phase and dilated cardiomyopathy phase were detected on the 21st day and 56th day in each group of mice, respectively.
[0046] 2) The mouse heart was fixed and embedded in paraffin, and HE staining was used to evaluate the inflammato...
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