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Compound probiotics capable of relieving ulcerative colitis, preparation method and application thereof

A technology for ulcerative colitis and compound probiotics, applied in biochemical equipment and methods, microorganism-based methods, bacteria used in food preparation, etc., to improve colonic mucosal inflammatory lesions, reduce colon weight-to-length ratio, The effect of reducing disease activity index

Active Publication Date: 2021-09-24
JIANGSU WECARE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that probiotics can enhance the integrity of tight junctions, thereby preventing damage to tight junctions by chemicals or harmful pathogens, but the treatment of UC with probiotics is still in the development stage

Method used

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  • Compound probiotics capable of relieving ulcerative colitis, preparation method and application thereof
  • Compound probiotics capable of relieving ulcerative colitis, preparation method and application thereof
  • Compound probiotics capable of relieving ulcerative colitis, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: Preparation of Bifidobacterium longum BL21 freeze-dried powder

[0050] Bifidobacterium longum BL21 was inoculated into the culture medium according to the inoculum amount of 3% of the total mass of the culture medium, and cultured at 37°C for 18 hours to obtain a culture solution; the culture solution was centrifuged to obtain cells; the cells trehalose Trehalose lyoprotectant with a concentration of 100g / L was resuspended (the mass ratio of lyoprotectant to bacteria was 2:1) to obtain a resuspension; the resuspension was lyophilized by vacuum freezing to obtain a long Bifidobacterium longum BL21 freeze-dried powder.

Embodiment 2

[0051] Example 2: Preparation of Bifidobacterium animalis subsp. lactisBLa80 freeze-dried powder

[0052] Inoculate Bifidobacterium animalis subsp. lactis BLa80 into the culture medium according to the inoculum amount accounting for 3% of the total mass of the culture medium, and cultivate it at 37°C for 18 hours to obtain a culture medium; centrifuge the culture medium to obtain bacteria ; Resuspend the bacteria with a trehalose lyoprotectant with a trehalose concentration of 100g / L (the mass ratio of the lyoprotectant to the bacteria is 2:1) to obtain a resuspension; vacuum freeze the resuspension freeze-drying method to obtain Bifidobacterium animalis subsp. lactis BLa80 freeze-dried powder.

Embodiment 3

[0053] Embodiment 3: the preparation of Lactobacillus casei LC89 freeze-dried powder

[0054] Lactobacillus casei LC89 was inoculated into the culture medium according to the inoculation amount of 3% of the total mass of the culture medium, and cultured at 37°C for 18 hours to obtain a culture solution; the culture solution was centrifuged to obtain bacteria; the bacteria were treated with trehalose Trehalose lyoprotectant with a concentration of 100g / L was resuspended (the mass ratio of lyoprotectant to bacteria was 2:1) to obtain a resuspension; the resuspension was lyophilized by vacuum freezing to obtain cheese Lactobacillus casei LC89 lyophilized powder.

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Abstract

The invention discloses compound probiotics capable of relieving ulcerative colitis, a preparation method and application thereof, and relates to the technical field of microorganisms. The compound probiotics are characterized by comprising Bifidobacterium longum BL21, Bifidobacterium animalis subsp.lactis BLa80 and Lactobacillus casei LC89. The preparation method comprises the following steps of: respectively preparing freeze-dried powder of the Bifidobacterium longum BL21, the Bifidobacterium animalis subsp.lactis BLa80 and the Lactobacillus casei LC89; and uniformly mixing the three freeze-dried powder in proportion to prepare the compound probiotics. The compound probiotics provided by the invention can prevent and / or treat ulcerative colitis, and have a great application prospect in preparation of products (such as solid beverages) for preventing and / or treating ulcerative colitis.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a compound probiotic capable of alleviating ulcerative colitis, a preparation method and application thereof. Background technique [0002] Ulcerative colitis (UC) is a chronic non-specific inflammatory bowel disease. The lesions mainly involve the mucosa and submucosa. The lesions mostly start from the sigmoid colon and rectum, and develop backward from the distal end, and can involve the entire colon and even the terminal ileum. , the treatment time is long, and it is easy to relapse, and the long course of the disease can easily lead to canceration, which seriously affects the quality of life and life and health of patients. Its specific pathogenesis is still unclear, and it is believed that its pathogenesis is related to various factors such as genetic factors, environmental factors, intestinal mucosal barrier dysfunction, and infection factors. At present, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L2/38A23L2/52A23L33/135A23L33/21A61K35/745A61K35/747A61P1/00A61P1/04A61P29/00C12R1/245C12R1/01
CPCC12N1/20A23L33/135A23L33/21A23L2/38A23L2/52A61K35/747A61K35/745A61P1/04A61P1/00A61P29/00A23V2002/00A23V2400/125A23V2400/515A23V2400/533A23V2200/3204A23V2200/3202A23V2250/5062A61K2300/00Y02A50/30
Inventor 方曙光盖忠辉朱建国吴明科吴银琴
Owner JIANGSU WECARE BIOTECHNOLOGY CO LTD
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