Microcosm culture device and application thereof in quantitative analysis of soil carbon diffusion and microorganism utilization process

A technology for culturing devices and microorganisms, applied in enzymology/microbiology devices, bioreactor/fermenter combinations, bioreactors/fermenters for specific purposes, etc. The effect of bioavailability

Active Publication Date: 2021-09-28
BEIJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004]Based on the basis that the carbon source-microbe space distance is an important factor affecting the utilization rate of soil organic carbon, scholars at home and abroad have established the Microbial-Mineral Carbon Stabilizatio

Method used

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  • Microcosm culture device and application thereof in quantitative analysis of soil carbon diffusion and microorganism utilization process

Examples

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Embodiment 1

[0038] The present invention provides a microcosm culture device, such as figure 1 As shown, it includes a closed container 1 and an incubator 2 and a dialysis tube 3 in the closed container 1;

[0039] The incubator 2 includes a soil layer 4;

[0040] The dialysis tube 3 is connected to the incubator 2 , and part of the tube extends through the side wall of the incubator 2 into the soil layer 4 along the length direction.

[0041] The airtight container 1 can be selected from various airtight containers commonly used in the art, as long as the airtightness is maintained, such as a jar with a cap.

[0042] Further, the dialysis tube 3 passes through the two side walls of the incubator 2;

[0043] Preferably, the dialysis tube 3 passes through the side walls of two opposite sides of the incubator 2;

[0044] More preferably, the dialysis tubing 3 is parallel to the non-passed side walls, and has the same distance from the two non-passed side walls. In the case of the same d...

Embodiment 2

[0053] Based on the microcosm culture device provided in Example 1, this example provides a quantitative analysis method for soil carbon diffusion and microbial utilization, which specifically includes the following process:

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Abstract

The invention relates to the field of soil process analysis, in particular to a microcosm culture device and application thereof in quantitative analysis of soil carbon diffusion and microorganism utilization processes. The microcosm culture device comprises a closed container as well as a culture box and a dialysis tube which are positioned in the closed container, the incubator comprises a soil layer; the dialysis tube is connected with the incubator, part of the tube body penetrates through the side wall of the incubator in the length direction and extends into the soil layer, a carbon source is contained in the dialysis tube, the dialysis tube can enable the carbon source to be diffused to the soil layer, and the water potential inside and outside the dialysis tube is always kept consistent. The quantitative analysis method for the soil carbon diffusion and microorganism utilization process is provided on the basis of the microcosm culture device, the relationship between the efficiency of utilizing the exogenous carbon source by the microorganisms and the space can be explored through the method, and the influence of the carbon diffusion distance on the efficiency of utilizing the exogenous carbon by the microorganisms is further quantitatively analyzed.

Description

technical field [0001] The invention relates to the field of soil process analysis, in particular to a microcosm cultivation device and its application in the quantitative analysis of soil carbon diffusion and microbial utilization process. Background technique [0002] As the main source and sink, soil organic carbon accounts for about 58% of the total soil carbon. In the current study, researchers often focus on carbon sequestration, where carbon in plants is fixed as soil organic carbon. In recent years, studies have shown that this process is not a simple polymerization of monomers into complex bodies, but a complex process involving physical and chemical interactions at the molecular level. In addition to carbon sequestration, carbon utilization has gradually become a focus of attention. [0003] Part of the carbon in the soil can be directly used by microorganisms, and the other part needs to be used by chemical reactions. However, a large part of carbon sources cann...

Claims

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Application Information

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IPC IPC(8): C12M1/00G01N33/00G01N33/24
CPCC12M29/04G01N33/0004G01N33/24C12M41/30C12M41/34
Inventor 虞璐
Owner BEIJING UNIV OF TECH
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