Microcosm culture device and application thereof in quantitative analysis of soil carbon diffusion and microorganism utilization process

Abstract

The invention relates to the field of soil process analysis, in particular to a microcosm culture device and application thereof in quantitative analysis of soil carbon diffusion and microorganism utilization processes. The microcosm culture device comprises a closed container as well as a culture box and a dialysis tube which are positioned in the closed container, the incubator comprises a soil layer; the dialysis tube is connected with the incubator, part of the tube body penetrates through the side wall of the incubator in the length direction and extends into the soil layer, a carbon source is contained in the dialysis tube, the dialysis tube can enable the carbon source to be diffused to the soil layer, and the water potential inside and outside the dialysis tube is always kept consistent. The quantitative analysis method for the soil carbon diffusion and microorganism utilization process is provided on the basis of the microcosm culture device, the relationship between the efficiency of utilizing the exogenous carbon source by the microorganisms and the space can be explored through the method, and the influence of the carbon diffusion distance on the efficiency of utilizing the exogenous carbon by the microorganisms is further quantitatively analyzed.

Description

technical field [0001] The invention relates to the field of soil process analysis, in particular to a microcosm cultivation device and its application in the quantitative analysis of soil carbon diffusion and microbial utilization process. Background technique [0002] As the main source and sink, soil organic carbon accounts for about 58% of the total soil carbon. In the current study, researchers often focus on carbon sequestration, where carbon in plants is fixed as soil organic carbon. In recent years, studies have shown that this process is not a simple polymerization of monomers into complex bodies, but a complex process involving physical and chemical interactions at the molecular level. In addition to carbon sequestration, carbon utilization has gradually become a focus of attention. [0003] Part of the carbon in the soil can be directly used by microorganisms, and the other part needs to be used by chemical reactions. However, a large part of carbon sources cann...

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Problems solved by technology

[0004]Based on the basis that the carbon source-microbe space distance is an important factor affecting the utilization rate of soil organic carbon, scholars at home and abroad have established the Microbial-Mineral Carbon Stabilization (MIMIC ) calculation model, or establish a substrate-microbe / microbe-substrate conceptual model, however, there is a lack of relevant methods and devices for quantitative research on soil organic carbon utilization by microorganisms

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