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Xanthine oxidase inhibitor as well as screening method and application thereof

A technology of xanthine oxidase and screening method, which is applied in the field of xanthine oxidase inhibitors and its screening, and can solve problems such as complex chemical components

Active Publication Date: 2021-10-01
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical composition of the sunflower disk extract is complex, and there is no research on the chemical composition of the sunflower disk extract as uric acid-lowering and anti-gout active substances

Method used

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  • Xanthine oxidase inhibitor as well as screening method and application thereof
  • Xanthine oxidase inhibitor as well as screening method and application thereof
  • Xanthine oxidase inhibitor as well as screening method and application thereof

Examples

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Effect test

preparation example Construction

[0031] In the present invention, the sunflower plate volatile oil is preferably self-made, and the preparation method of the sunflower plate volatile oil preferably includes the following steps: mixing sunflower plate powder, sodium chloride and water, and extracting to obtain the sunflower plate volatile oil.

[0032] In the present invention, the mass ratio of the sunflower disc powder to sodium chloride is preferably 1:0.5-1, more preferably 1:0.75; the mass ratio of the sunflower disc powder to water is preferably 1:10-20, More preferably 1:15. In the present invention, the sunflower disc powder is preferably obtained by crushing sunflower discs and drying them; the present invention has no special limitation on the source of the sunflower discs, and the sunflower discs well-known to those skilled in the art can be used. At least one of LD5009, edible sunflower SH363 and oil sunflower S606; the present invention has no special limitation on the pulverization method, and it...

Embodiment 1

[0048] (1) Extraction of volatile oil from sunflower dish

[0049] The sunflower disc of Shikui LD5009 was crushed and then dried to obtain sunflower disc powder. Put 100g of sunflower disc powder into a 2000mL beaker, add 1500mL of water and 75gNaCl, place the beaker in an ultrasonic pot for ultrasonication for 1h, transfer it to a volatile oil extractor, and distill and extract for 8h to obtain the extract; After ether extraction, the gained organic phase was dried with sodium sulfate and then concentrated by a rotary evaporator to remove petroleum ether to obtain the volatile oil of LD5009 sunflower dish (brownish yellow), and the extraction rate of volatile oil of LD5009 sunflower dish was 0.42%. Store in refrigerator at 4°C until use.

[0050] (2) Identification of chemical components of volatile oil from sunflower pan

[0051] The sunflower disc volatile oil was diluted with n-hexane, and then analyzed by GC-MS with Agilent5975 (Agilent, USA). Among them, the conditio...

Embodiment 2

[0053] Prepare sunflower plate volatile oil according to the method of Example 1 and analyze and identify its chemical composition. The difference from Example 1 is that the food sunflower LD5009 is replaced by food sunflower SH363, and the chemical composition analysis results of the food sunflower SH363 sunflower plate volatile oil are as follows figure 2 and shown in Table 1.

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Abstract

The invention provides a xanthine oxidase (XO) inhibitor as well as an extraction method and application thereof, and belongs to the technical field of medicines. The xanthine oxidase inhibitor provided by the invention is prepared from at least two of eupatoriochromene, cumyl alcohol and 1, 5, 8-p-menthatriene. The XO inhibitor provided by the invention can form a Pi-alkyl group and a Pi-Pi conjugated double bond with an active center of XO, eupatochromene and cumyl alcohol can form a Van der Waals force with the active center of XO, and in the presence of the intermolecular acting force, the XO inhibitor is inserted into hydrophobic gaps of XO to occupy a catalytic active center of the XO, so that the substrate is prevented from entering and the original function of the substrate is lost. TheXO inhibitor has the effect of inhibiting the production of uric acid, indicating that eupatoriochromene, cumyl alcohol and 1, 5, 8-p-menthatriene have the function of inhibiting XO. A new thought is provided for screening the XO inhibitor from natural products to prepare medicines for treating XO-mediated diseases.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a xanthine oxidase inhibitor and its screening method and application. Background technique [0002] Gout is a metabolic disease caused by purine metabolism disorder or excessive production of uric acid in the body and decreased excretion in vitro. Clinical manifestations include hyperuricemia, gouty arthritis, uric acid stones, gout stone formation, etc. Hyperuricemia is a stage in the development of gout; gouty arthritis is a series of inflammatory reactions caused by the deposition of uric acid in joint tissues after the serum uric acid level increases. [0003] The main cause of gout is: when the production of uric acid in the body increases or the excretion decreases, it can lead to an increase in the level of uric acid. When it exceeds its dissolution limit, uric acid will be deposited in the joints and soft tissues, causing an inflammatory response. Uric acid is the fin...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61K31/045A61K31/015A61P19/06A61P3/10A61P35/00G01N30/02G01N30/72A61K36/28
CPCA61K31/352A61K31/045A61K31/015A61P19/06A61P3/10A61P35/00G01N30/02G01N30/72A61K36/28
Inventor 韩璐刘新胜韩葳葳董占德高波李婉南
Owner JILIN UNIV
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