Method for separating mogroside V and rare mogroside substances
A mogroside and separation method technology, applied in chemical instruments and methods, organic chemistry, glycoside steroids, etc., can solve the problems of difficult control, large scale difficulty, complicated process, etc., to achieve strong practicability and ensure adsorption accuracy. and sufficiency effects
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[0076] The present invention does not limit the source and preparation method of mogroside, which can be purchased from the market or prepared by well-known preparation methods in the art. In order to ensure the separation effect, the total content of rare sweet glycosides in the mogroside is ≥ 10%, and the content of sweet glycoside V is ≥ 50%. Preferably, the total content of said rare sweet glycosides is ≥ 25%, and the content of sweet glycosides ≥ 65%. In the mogroside rich in rare sweet glycosides used in the examples of the present invention, the sweet glycoside contents are: mogroside V 62.2%, mogroside II E 3.4%, simonoside I 6.3%, 11-O glycoside V18.8% in. The preparation method of the mogroside mainly includes the steps of extraction, clarification, adsorption purification, and desalting and decolorization. Specifically, the method for preparing the mogroside is as follows:
[0077] a) Leaching: Leach fresh Luo Han Guo in continuous countercurrent equipment with p...
Embodiment 1
[0091] The preparation method of mogroside is as follows:
[0092] (1) Lixiviation: take fresh Luo Han Guo, carry out utilizing continuous countercurrent equipment lixiviation under the condition of 90 ℃ pure water, solid-liquid ratio (mass) as 1: 3, make initial extract (per 10 tons of fresh Luo Han Guo make 28 tons of initial extraction liquid);
[0093] (2) clarification: then pass the prepared initial liquid through a disc centrifuge to obtain a light liquid, pass through an ultrafiltration membrane of 1 to 50,000 Da, and collect the clarified permeate (collected per 28 tons of initial liquid) Obtain 25 tons of permeate);
[0094] (3) Primary adsorption purification: put the permeate on the macroporous adsorption resin D101 chromatographic column, and desorb with 3BV, 20% ethanol aqueous solution and 2.5BV, 65% ethanol aqueous solution respectively, and the desorption flow rate is 1BV / h , the desorption components of the 65% ethanol aqueous solution were combined, and af...
Embodiment 2
[0098] The imprinted polymers were prepared as follows:
[0099] Weigh the rare sweet glycoside monomer standard (mogroside IIE 83.2%, simanoside I 85.5%, 11-O glycoside V91.5%) as template molecules and fully dissolve 60ml of methacrylic acid in 2500ml porogen ( N,N-Dimethylformamide:Acetonitrile volume fraction ratio is 1:4) in the Erlenmeyer flask, then place it at a constant temperature of 30°C and shake it well for 1h, then add 150ml of ethylene glycol Alcohol dimethacrylate and 1000ml of azobisisobutyronitrile, sealed with nitrogen gas for 20 minutes, polymerized at 60°C for 24 hours, taken out and dried, crushed, and ground through a 120-mesh sieve to obtain mogroside IIE respectively Column packing, symmonoside I column packing and 11-O glycoside V column packing.
[0100] Put the prepared three packing materials into sealable chromatographic columns respectively, with a diameter-to-height ratio of 1: (6-7), to prepare mogroside IIE column, simonoside I column and 11-...
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