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Method for separating mogroside V and rare mogroside substances

A mogroside and separation method technology, applied in chemical instruments and methods, organic chemistry, glycoside steroids, etc., can solve the problems of difficult control, large scale difficulty, complicated process, etc., to achieve strong practicability and ensure adsorption accuracy. and sufficiency effects

Active Publication Date: 2021-10-01
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the prior art, some methods for preparing mogrosides from Mogrosides often have various shortcomings. For example, although some solutions can enrich high levels of mogrosides, they have high requirements for equipment, and in the collection stage Using segmented collection, this technology is difficult to control and difficult to achieve large-scale production
There are also some schemes that can only enrich one kind of sweet glycosides, but cannot effectively separate other sweet glycosides
There are other solutions that require a large amount of organic solvents in the extraction process. Not only is the process cumbersome and difficult to achieve large-scale production, but more importantly, the use of organic solvents makes it difficult to apply the product to food or health care products.
There are also some programs that can obtain different types of mogrosides through extraction, concentration, macroporous resin purification, desorption, MCI column chromatography purification, gradient elution, segmented collection, chromatographic detection, concentration and drying, but the process requires At the same time of multiple adsorption purifications, it is also necessary to combine ladder elution and segmented collection, which is cumbersome to operate
[0006] It can be seen that most of the existing methods use gradient elution, segmented collection or crystallization to separate various sweet glycosides. This requires a high enough accuracy for the immediacy and accuracy of the detection, so it directly makes it extremely difficult to achieve large-scale

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  • Method for separating mogroside V and rare mogroside substances

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preparation example Construction

[0076] The present invention does not limit the source and preparation method of mogroside, which can be purchased from the market or prepared by well-known preparation methods in the art. In order to ensure the separation effect, the total content of rare sweet glycosides in the mogroside is ≥ 10%, and the content of sweet glycoside V is ≥ 50%. Preferably, the total content of said rare sweet glycosides is ≥ 25%, and the content of sweet glycosides ≥ 65%. In the mogroside rich in rare sweet glycosides used in the examples of the present invention, the sweet glycoside contents are: mogroside V 62.2%, mogroside II E 3.4%, simonoside I 6.3%, 11-O glycoside V18.8% in. The preparation method of the mogroside mainly includes the steps of extraction, clarification, adsorption purification, and desalting and decolorization. Specifically, the method for preparing the mogroside is as follows:

[0077] a) Leaching: Leach fresh Luo Han Guo in continuous countercurrent equipment with p...

Embodiment 1

[0091] The preparation method of mogroside is as follows:

[0092] (1) Lixiviation: take fresh Luo Han Guo, carry out utilizing continuous countercurrent equipment lixiviation under the condition of 90 ℃ pure water, solid-liquid ratio (mass) as 1: 3, make initial extract (per 10 tons of fresh Luo Han Guo make 28 tons of initial extraction liquid);

[0093] (2) clarification: then pass the prepared initial liquid through a disc centrifuge to obtain a light liquid, pass through an ultrafiltration membrane of 1 to 50,000 Da, and collect the clarified permeate (collected per 28 tons of initial liquid) Obtain 25 tons of permeate);

[0094] (3) Primary adsorption purification: put the permeate on the macroporous adsorption resin D101 chromatographic column, and desorb with 3BV, 20% ethanol aqueous solution and 2.5BV, 65% ethanol aqueous solution respectively, and the desorption flow rate is 1BV / h , the desorption components of the 65% ethanol aqueous solution were combined, and af...

Embodiment 2

[0098] The imprinted polymers were prepared as follows:

[0099] Weigh the rare sweet glycoside monomer standard (mogroside IIE 83.2%, simanoside I 85.5%, 11-O glycoside V91.5%) as template molecules and fully dissolve 60ml of methacrylic acid in 2500ml porogen ( N,N-Dimethylformamide:Acetonitrile volume fraction ratio is 1:4) in the Erlenmeyer flask, then place it at a constant temperature of 30°C and shake it well for 1h, then add 150ml of ethylene glycol Alcohol dimethacrylate and 1000ml of azobisisobutyronitrile, sealed with nitrogen gas for 20 minutes, polymerized at 60°C for 24 hours, taken out and dried, crushed, and ground through a 120-mesh sieve to obtain mogroside IIE respectively Column packing, symmonoside I column packing and 11-O glycoside V column packing.

[0100] Put the prepared three packing materials into sealable chromatographic columns respectively, with a diameter-to-height ratio of 1: (6-7), to prepare mogroside IIE column, simonoside I column and 11-...

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Abstract

The invention relates to the technical field of active substance separation, in particular to a method for separating mogroside V and rare mogroside substances. The method comprises the following steps: dissolving mogroside, and degassing the solution to obtain a sampling solution; loading the sampling liquid onto a multi-cascade chromatographic column formed by connecting an imprinted polymer chromatographic column and a macroporous adsorption resin chromatographic column in series, and sequentially washing the column with a degassed ethanol water solution and a degassed water washing column; releasing vacuum to normal pressure, and respectively desorbing the disconnected imprinted polymer chromatographic column with a mixed solution of acetonitrile and ethanol; respectively collecting desorption solutions containing rare mogroside substances; and desorbing the macroporous adsorption resin chromatographic column by using an ethanol aqueous solution, and collecting a desorption solution containing mogroside V. The mogroside collected by the method is high in yield and high in purity, and the chromatographic column can be recycled.

Description

technical field [0001] The invention relates to the technical field of active substance separation, in particular to a method for separating mogroside V and rare sweet glycoside substances. Background technique [0002] The botanical name of Luo Han Guo is "Jackia glabra", which is the mature fruit of the Cucurbitaceae Luo Han Guo plant. It is a unique economic and medicinal plant in my country, mainly distributed in Guangxi, Hunan, Guizhou and other regions. Luo Han Guo has high nutritional value and is rich in fructose, protein, amino acids and multivitamins. It is cool in nature, sweet in taste, and non-toxic. , chronic bronchitis, pharyngitis, and gastrointestinal diseases have a significant effect. Widely used in medicine, beverages and condiments. The sweet substance with extremely high sweetness contained in it is an ideal low-calorie natural sweetener. Studies have shown that the sweet substance of Luo Han Guo, that is, mogroside, is a cucurbitane triterpene glyco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 何安乐黄华学刘庚贵熊瑶黄俊
Owner HUNAN HUACHENG BIOTECH
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