Application of deubiquitinating enzyme USP28 in preparation of medicine for preventing or treating pancreatic cancer

A technology for deubiquitinating enzymes and pancreatic cancer, which can be used in drug combinations, biochemical equipment and methods, enzymes, etc., and can solve the problems of unclear biological functions and expression patterns of pancreatic cancer.

Pending Publication Date: 2021-10-15
THE SECOND AFFILIATED HOSPITAL TO NANCHANG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the biological functions and expression pa

Method used

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  • Application of deubiquitinating enzyme USP28 in preparation of medicine for preventing or treating pancreatic cancer
  • Application of deubiquitinating enzyme USP28 in preparation of medicine for preventing or treating pancreatic cancer
  • Application of deubiquitinating enzyme USP28 in preparation of medicine for preventing or treating pancreatic cancer

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Embodiment 1

[0039] Materials and Method

[0040] Patient and tumor specimens

[0041] Matching pancreatic cancer and normal pancreatic tissue were taken from 102 cases of pancreatic cancer patients in General Surgery of the Second Affiliated Hospital of Nanchang University in 2015-2019. The removal specimen immediately placed in liquid nitrogen, and stored at -80 ° C for further analysis. All patients have gained written informed consent, and the research procedures were approved by the Ethics and Research Committee of the Second Affiliated Hospital of Nanchang University. Table 1 summarizes the clinical features of all patients.

[0042] Cell line and cell culture

[0043] The normal pancreatic catheter epithelial cell line (H6C7 was used as a control) and a pancreatic cancer cell line (ASPC-1, PANC-1, BXPC-3 and SW1990) purchased from the American Typical Casting Center (Virginia, Virginia, USA). The cell line was cultured under the addition of 10% fetal bovine serum and 100 units / ml peni...

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Abstract

The invention discloses an application of deubiquitinating enzyme USP28 in preparation of a medicine for preventing or treating pancreatic cancer. Research finds that USP28 expression in pancreatic cancer tumor tissue is higher than that in normal pancreatic tissue, and USP28 high expression is remarkably related to malignant phenotype and lifetime shortening of pancreatic cancer patients; overexpression of the USP28 can accelerate growth of pancreatic cancer cells, and down-regulation of the USP28 can inhibit in-vitro and in-vivo growth of the pancreatic cancer cells; USP28 promotes the growth of pancreatic cancer cells by accelerating the cell cycle process and inhibiting cell apoptosis. In the aspect of mechanism, USP28 is subjected to ubiquitination and stabilizes a transcription factor FOXM1, which is a key medium for Wnt/beta-catenin signal transduction, USP28-mediated FOXM1 stabilizes and significantly promotes beta-catenin nucleation so as to further cause activation of a Wnt/beta-catenin pathway, and recovery of FOXM1 expression can alleviate the antitumor effect caused by down regulation of USP28. The deubiquitinating enzyme USP28 promotes the development of the pancreatic cancer by enhancing FOXM1 mediated Wnt/beta-catenin signal channel activation, and a powerful means is provided for potential targeted treatment and prevention of pancreatic cancer patients in the future.

Description

Technical field [0001] The present invention relates to the study of the pathogenesis of pancreatic cancer, and more particularly to the use of a prophylactinization USP28 in the preparation of drugs for preventing or treating pancreatic cancer, more specifically, to de-ubiquitinization USP28 by stabilizing FOXM1 activation The mechanism of WNTβ-Catenin promotes the growth of pancreatic cancer and its use in preventing or treating pancreatic cancer. Background technique [0002] Pancreatic Cancer (PC) is the seventh reason why global cancer related deaths. Its prognosis is mainly reflected in about 5% of the 5-year survival rate, mainly due to diagnosis of late and lack of effective treatment. Therefore, it is an urgent need to determine the new target and development of new target and development of new target and development to treat new treatment strategies for pancreatic cancer. [0003] Changes in protein ubiquitinization are usually associated with tumors. DUBS cancels the ...

Claims

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Application Information

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IPC IPC(8): A61K38/48A61P1/18A61P35/00
CPCA61K38/4873C12Y304/22A61P1/18A61P35/00
Inventor 彭小刚陈磊峰徐正邹叶青邵俊
Owner THE SECOND AFFILIATED HOSPITAL TO NANCHANG UNIV
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