Human renal angiomyolipoma PCDX mouse model and establishment method thereof

Abstract

The invention discloses a human renal angiomyolipoma PCDX mouse model and an establishment method thereof, and belongs to the field of medical fundamental research. The human renal angiomyolipoma PCDX mouse model is obtained by subcutaneously inoculating human renal angiomyolipoma primary cells on the shoulder and back of the forelimb of a mouse and then culturing; and the human renal angiomyolipoma primary cells have been preserved in China Center for Type Culture Collection in advance. The establishment method of the mouse model comprises the following steps: taking the human renal angiomyolipoma primary cells, and conducting culturing, proliferating and digesting to obtain a sufficient primary cell suspension; preparing a primary cell mixed solution; and inoculating the primary cell mixed solution to the subcutaneous part of the back of the forelimb of the mouse, and feeding the mouse in an SPF environment to obtain the human renal angiomyolipoma PCDX mouse model. The human renal angiomyolipoma PCDX mouse model is established for the first time, and a model foundation is laid for basic clinical research of the PCDX mouse model. The establishment method of the mouse model is high in tumor formation speed, high in tumor formation rate and low in mouse death rate; and one-time large-scale preparation and repeated modeling can be realized.

Description

technical field [0001] The invention belongs to the field of basic medical research, in particular to a mouse model of human renal angiomyolipoma and a method for establishing the same. Background technique [0002] According to the 2016 WHO classification of renal tumors, renal angiomyolipoma (RAML) is classified as a mesenchymal tumor that mainly occurs in adults. Histologically, there are two types of RAML, one is classical RAML and the other is epithelioid RAML. Classic RAML is a benign mesenchymal neoplasm composed of adipose tissue, spindle-shaped and epithelioid smooth muscle cells, and thick-walled vessels. Epithelioid RAML is characterized by the proliferation of epithelioid cells on the basis of classical RAML structure, showing infiltrating and destructive growth, and is a mesenchymal tumor with malignant potential. RAML accounts for 8.7%-29.4% of renal tumors. Because it can lead to common complications of spontaneous rupture and bleeding, it can cause hemorrh...

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Problems solved by technology

[0003] Although a series of studies in the field of tumors have been carried out extensively, and the research fields involve many aspects such as tumor diagnosis, pathogenesis and drug treatment, the pathogenesis of human renal angiomyolipoma is still not clear, the main reason is that the current market There are no suitable animal models of human renal angiomyolipoma available for the study of the disease

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