Human renal angiomyolipoma PCDX mouse model and establishment method thereof

A technology of vascular smooth muscle and mouse model, applied in the field of medical basic research, can solve the problem that the pathogenesis of human renal angiomyolipoma is not yet clear, and achieve the effect of fast tumor formation, high tumor formation rate and low mortality

Active Publication Date: 2021-10-26
武汉赛尔朗灵科技有限公司
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Problems solved by technology

[0003] Although a series of studies in the field of tumors have been carried out extensively, and the research fields involve many aspects such as tumor diagnosis, pathogenesis and drug treatment, the patho

Method used

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  • Human renal angiomyolipoma PCDX mouse model and establishment method thereof
  • Human renal angiomyolipoma PCDX mouse model and establishment method thereof
  • Human renal angiomyolipoma PCDX mouse model and establishment method thereof

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Embodiment 1

[0034] Example 1: Human Renal Angiomyolipoma Primary Cell Subcutaneous Tumor Formation Test in Nude Mice

[0035] The primary human renal angiomyolipoma cell XHRAML-06 used in this example was deposited by the applicant on December 25, 2020 at the Chinese Type Culture Collection Center of Wuhan University, Wuhan, China, and the deposit number is CCTCC NO: C202082 , the selected mice are commercially available SPF grade BALB / C Nude Crlj mice (purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.), other selected raw materials and reagents are commercially available, and the specific model establishment method for:

[0036] S1. Take XHRAML-06 primary human renal angiomyolipoma cells, inoculate them in T5 cell culture flasks for culture and proliferation. After proliferation and culture until the cell abundance reaches 90%, use Celigo to calculate and record the number of cells, use 3ml Digest with trypsin at a concentration of 0.05%, and finally add 3 ml...

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Abstract

The invention discloses a human renal angiomyolipoma PCDX mouse model and an establishment method thereof, and belongs to the field of medical fundamental research. The human renal angiomyolipoma PCDX mouse model is obtained by subcutaneously inoculating human renal angiomyolipoma primary cells on the shoulder and back of the forelimb of a mouse and then culturing; and the human renal angiomyolipoma primary cells have been preserved in China Center for Type Culture Collection in advance. The establishment method of the mouse model comprises the following steps: taking the human renal angiomyolipoma primary cells, and conducting culturing, proliferating and digesting to obtain a sufficient primary cell suspension; preparing a primary cell mixed solution; and inoculating the primary cell mixed solution to the subcutaneous part of the back of the forelimb of the mouse, and feeding the mouse in an SPF environment to obtain the human renal angiomyolipoma PCDX mouse model. The human renal angiomyolipoma PCDX mouse model is established for the first time, and a model foundation is laid for basic clinical research of the PCDX mouse model. The establishment method of the mouse model is high in tumor formation speed, high in tumor formation rate and low in mouse death rate; and one-time large-scale preparation and repeated modeling can be realized.

Description

technical field [0001] The invention belongs to the field of basic medical research, in particular to a mouse model of human renal angiomyolipoma and a method for establishing the same. Background technique [0002] According to the 2016 WHO classification of renal tumors, renal angiomyolipoma (RAML) is classified as a mesenchymal tumor that mainly occurs in adults. Histologically, there are two types of RAML, one is classical RAML and the other is epithelioid RAML. Classic RAML is a benign mesenchymal neoplasm composed of adipose tissue, spindle-shaped and epithelioid smooth muscle cells, and thick-walled vessels. Epithelioid RAML is characterized by the proliferation of epithelioid cells on the basis of classical RAML structure, showing infiltrating and destructive growth, and is a mesenchymal tumor with malignant potential. RAML accounts for 8.7%-29.4% of renal tumors. Because it can lead to common complications of spontaneous rupture and bleeding, it can cause hemorrh...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/0271A01K2207/12A01K2227/105A01K2267/0331
Inventor 刘红亚刘霞王前进李俊俊李继新
Owner 武汉赛尔朗灵科技有限公司
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