Aminoethyl biphenyl compound as well as preparation method and application thereof
A technology of aminoethylbiphenyl and ethylbiphenyl, which is applied in the field of medicine, can solve the problems of less chemical components and pharmacological effects of pseudophyllum, and achieve the effect of inhibiting tumor cell proliferation
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Embodiment 1
[0050] Embodiment 1: extract aminoethyl biphenyl compound from pseudoleaf tree
[0051] 1.1 Experimental materials
[0052] The false leaf tree was purchased in Anguo Market, Hebei Province in June 2016. The plant was identified by Professor Ning Wei of the College of Horticulture, Shenyang Agricultural University, and the plant samples were preserved in the Animal Pharmacy Experimental Center of Shenyang Agricultural University.
[0053] 1.2 Test method
[0054] a) 2.5kg of the rhizome of the pseudoleaf tree was extracted with 20L 95% ethanol at 80°C under reflux, the extraction was repeated 3 times, the obtained extracts were combined, and concentrated under reduced pressure to obtain 61g of the extract;
[0055] b) Suspend the extract obtained in step a) in water, extract with n-hexane, ethyl acetate, and n-butanol in sequence, each solvent is according to the volume ratio of the aqueous phase and the organic phase 1:1, to obtain ethyl acetate Extract;
[0056] c) The et...
Embodiment 2
[0067] Example 2: Aculebiphenyl A inhibits the proliferation of human leukemia cell lines
[0068] 2.1 Experimental materials
[0069] aculebiphenyl A (extracted and isolated from Pseudomonas japonica); human acute myeloblastic leukemia cell line HL-60, human chronic myeloid leukemia cell line K562, and human acute monocytic leukemia cell line THP-1 were provided by Shenyang Pharmaceutical University Huahui Donated by Professor Ming; fetal bovine serum, penicillin, streptomycin and RPMI1640 were purchased from Invitrogen, USA; 5-fluorouracil was purchased from Sigma, USA.
[0070] 2.2 Test method
[0071] HL-60, K562, and THP-1 cells were cultured in RPMI1640 medium containing 10% heat-inactivated fetal bovine serum, 100 IU / mL penicillin, 100 μg / mL streptomycin, and 1 mmol / L L-glutamine. 37°C, 5% CO 2 Incubate in a saturated humidity incubator and use it when the cell tile area reaches 70%-80%.
[0072] 0.4% trypan blue solution: weigh trypan blue, add a small amount of di...
Embodiment 3
[0079] Embodiment 3: Aculebiphenyl A antibacterial experiment
[0080] 3.1 Experimental materials
[0081] The tested strains Escherichia coli ATCC25922, Salmonella C7731, Staphylococcus aureus ATCC29213 and Streptococcus ATCC49619 were purchased from the Culture Collection Center of China Veterinary Drug Control Institute. The positive control drug gentamicin sulfate was purchased from Dalian Meilun Biotechnology Co., Ltd.
[0082] 3.2 Preparation of bacteria solution
[0083] Inoculate the preserved Staphylococcus aureus, Streptococcus, Escherichia coli, and Salmonella on the mannitol sodium chloride agar plate medium, the modified Edward agar plate medium, the MacConkey agar plate medium and the SS agar respectively in a streaking manner Incubate in plate culture medium at 37°C for 24 hours. Pick typical single colonies on the 4 kinds of culture medium and place them in nutrient broth (NB) culture medium, shake culture at 37°C and 150rpm until OD600=0.4-0.6, at this time...
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