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Tibetan anagrus cantoniensis odorant binding protein gene sequence and application thereof

An odor-binding protein and gene sequence technology, which is applied in the field of odor-binding protein gene sequences of H. tuftsiensis in Tibet, can solve problems such as affecting the biological control effect and increasing the control cost of walnut households, and achieves the enrichment of chemical ecology theory, enrichment The effects of new methods and approaches to control

Pending Publication Date: 2021-11-02
SOUTHWEST FORESTRY UNIVERSITY
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Problems solved by technology

Although the use of biological control can achieve better control effect, the feeding and protection of its natural enemies will undoubtedly increase the cost of control for walnut households, especially the change of season will lead to the migration of birds, which will affect the effect of biological control

Method used

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  • Tibetan anagrus cantoniensis odorant binding protein gene sequence and application thereof
  • Tibetan anagrus cantoniensis odorant binding protein gene sequence and application thereof
  • Tibetan anagrus cantoniensis odorant binding protein gene sequence and application thereof

Examples

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Embodiment

[0027] 1. Construction of the cDAN library of the Tibetan tassel-horned elephant beetle

[0028] 1.1 Use the Trizol method to extract the total RNA of the antennae, head, male, abdomen, foot, and wing of the male and female adults of the Tibetan tamarind beetle respectively. After the extracted total RNA samples pass the test, use magnetic beads carrying Oligo (dT) Enrich eukaryotic mRNA; then add fragmentation buffer to break mRNA into short fragments, use mRNA as a template, and use six-base random primers to synthesize one-strand cDNA, and then use DNA polymerase to synthesize second-strand cDNA; use AMPure After purification of the double-stranded cDNA by XP beads, end repair is performed, A-tails are added to connect the sequencing adapters, and the fragment size is selected with AMPure XP beads; PCR amplification is performed, and the PCR-amplified products are purified with AMPure XP beads to obtain the final library. After the library is constructed, use Qubit2.0 for p...

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Abstract

The invention discloses a Tibetan anagrus fasciculatus odorant binding protein gene sequence which comprises an OBP6 gene sequence and an OBP10 gene sequence, the OBP6 gene sequence is selected from SEQ ID No.1, and the OBP10 gene sequence is selected from SEQ ID No.2. The invention also provides application of the odor binding protein compiled by the OBP6 gene sequence and the OBP10 gene sequence in identification of odor compounds. The invention also discloses an application of the odorant binding protein compiled by the OBP6 gene sequence in identification of an odorant compound trans-2-hexenal. The invention also discloses an application of the odorant binding protein compiled by the OBP10 gene sequence in identification of odorant compound geraniol. According to the invention, through experiments, an attacked object (walnut) is positioned by combining protein compiled by OBP6 and OBP10 gene sequences with an odorant, so that a corresponding attractant can be developed to attract the Tibetan anagrus cantoniensis to a capture area.

Description

technical field [0001] The present invention relates to the gene sequence of the odor binding protein from the Tibetan tamarind beetle, in particular to the application of the odorant binding protein gene sequence of the Tibetan tamarind beetle in identifying walnut odor compounds. Background technique [0002] C. cretifera thibetana belongs to the order Coleoptera Coleoptera, the family Cerambycidae, the subfamily Lamiinae, the genus Cacia, and is mainly distributed in Yunnan, Sichuan, Guangxi, and Tibet Autonomous Region in my country. One of the serious dry-boring pests of commercial forest trees such as walnut in Yunnan. The worm emerges and migrates through the boreholes of adults at the end of April, lays eggs in May, and larvae begin to bore into the trunk at the end of June. It survives the winter as larvae until it emerges as adults, and the adults live in the trunk until the boreholes migrate (Ji Bao Medium, 2002). Adults will carve grooves on the walnut bark, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C12N15/12
CPCC07K14/43563
Inventor 赵宁杨斌毛祥忠
Owner SOUTHWEST FORESTRY UNIVERSITY
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