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A kind of electrochemical biosensor for UDG detection and detection method thereof
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A biosensor and detection method technology, applied in the field of electrochemical biosensor for UDG detection and its detection, can solve the problems of high cost and poor stability, and achieve the effects of low detection limit, excellent analytical performance, and reduced transmission length
Active Publication Date: 2022-08-02
SHANDONG UNIV
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However, the inventors found that these methods often require the participation of natural enzymes to amplify the signal and improve sensitivity, but the natural enzymes also bring new problems, such as high cost and poor stability
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[0031] In an embodiment of the present disclosure, the preparation method of the hollow Mn / Ni layered double metal hydroxide includes: mixing SiO 2 , potassium bromate, acrylamide, NiCl 2 ·6H 2 O, MnCl 2 ·4H 2 O is sealed in a high-pressure reactor, and SiO is obtained by high-temperature reaction 2 @Mn / Ni LDHs; then, the SiO 2 @Mn / Ni LDHs are dispersed in a strong alkaline solution, and the hollow Mn / Ni layered double metal hydroxide is obtained by the etching reaction; or, the temperature of the high temperature reaction is 120-160°C, preferably 140°C; the reaction The time is 8-13h, preferably 10h. Controlling the reaction temperature and time has a certain influence on obtaining hollow Mn / Ni layered double metal hydroxides with higher conductivity. During the etching process, if the etching time is long, the structure will be broken, which is not conducive to obtaining an electrochemical sensor with higher performance.
[0032] In one embodiment of the present discl...
Embodiment 1
[0050] 1.1 Synthesis of SiO 2 @Mn / Ni LDHs and hollow Mn / Ni LDHs (h-Mn / Ni LDHs)
[0051] First, through the traditional Synthesis of SiO 2 Nanoparticles: Ethyl orthosilicate (5 mL), ultrapure water (11.6 mL), and ammonia (4.4 mL) were sequentially added to isopropanol (140 mL), and the resulting liquid mixture was stirred at room temperature for five hours and centrifuged Separated, washed with ethanol, and dried at 80 °C for 12 h to obtain SiO 2 solid.
[0052] SiO 2 Synthesis of @Mn / Ni LDHs: SiO was added to 18 mL of ultrapure water 2 Nanoparticles (80mg), Potassium Bromate (0.9g, 5.39mM), Acrylamide (0.8g, 11.25mM), NiCl 2 ·6H 2 O (0.12g, 0.5mM) and MnCl 2 ·4H 2 O (0.02g, 0.1mM), stirred at room temperature for 1 hour, sealed in an autoclave, reacted at 140°C for 10h, centrifuged, washed with water, and dried at 80°C overnight to obtain SiO 2 @Mn / Ni LDHs.
[0053] To prepare hollow Mn / Ni LDHs, SiO 2 @Mn / Ni LDHs (30mg) were dispersed in 30mL sodium hydroxide solu...
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Abstract
The present disclosure belongs to the technical field of electrochemical biosensors, and in particular relates to an electrochemical biosensor for UDG detection and a detection method thereof. The electrochemical biosensor comprises: a hollow Mn / Ni layered double metal hydroxide loaded with gold nanoparticles The compound was incubated with DNA1 and hairpin DNA. Electrochemical biosensors based on h‑Mn / Ni LDHs with cascaded signal amplification. The biosensor showed excellent analytical performance with a detection limit as low as 4.8 × 10 ‑4 UmL ‑1 . More importantly, this strategy provides a new avenue for the application of oxidase-like enzymes in electrochemical biosensors, which can be extended to sensitively detect various biomarkers for bioanalysis and clinical diagnosis.
Description
technical field [0001] The present disclosure belongs to the technical field of electrochemical biosensors, and in particular relates to an electrochemical biosensor for UDG detection and a detection method thereof. Background technique [0002] The information disclosed in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Nanozymes are enzymatically active nanomaterials, which have become powerful candidates for replacing natural enzymes due to their high stability, convenience in large-scale preparation, and flexibility in transformation. Most of the successfully prepared nanozyme-based biosensors are concentrated on peroxidase-like enzymes. Among them, H 2 O 2 It is a necessity and can be catalyzed to generate reactive oxygen...
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