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Electrochemical biosensor for UDG detection and detection method thereof

A biosensor and detection method technology, applied in the field of electrochemical biosensor for UDG detection and its detection, can solve the problems of high cost and poor stability, and achieve the effects of low detection limit, excellent analytical performance and improved catalytic activity

Active Publication Date: 2021-11-26
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the inventors found that these methods often require the participation of natural enzymes to amplify the signal and improve sensitivity, but the natural enzymes also bring new problems, such as high cost and poor stability

Method used

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  • Electrochemical biosensor for UDG detection and detection method thereof
  • Electrochemical biosensor for UDG detection and detection method thereof
  • Electrochemical biosensor for UDG detection and detection method thereof

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preparation example Construction

[0031] In one embodiment of the present disclosure, the preparation method of the hollow Mn / Ni layered double hydroxide comprises: SiO 2 , potassium bromate, acrylamide, NiCl 2 ·6H 2 O, MnCl 2 4H 2 O is sealed in a high-pressure reactor, and SiO is obtained by high-temperature reaction 2 @Mn / Ni LDHs; then, the SiO 2 @Mn / Ni LDHs is dispersed in a strong alkali solution, and the etching reaction is to obtain a hollow Mn / Ni layered double metal hydroxide; or, the temperature of the high temperature reaction is 120-160°C, preferably 140°C; the reaction The time is 8-13h, preferably 10h. Controlling the temperature and time of the reaction has a certain influence on obtaining the hollow Mn / Ni layered double hydroxide with higher conductivity. In the etching process, if the etching time is longer, the structure will be broken, which is not conducive to obtaining an electrochemical sensor with higher performance.

[0032] In one embodiment of the present disclosure, the method...

Embodiment 1

[0050] 1.1 Synthesis of SiO 2 @Mn / Ni LDHs and hollow Mn / Ni LDHs (h-Mn / Ni LDHs)

[0051] First, through the traditional Synthesis of SiO 2 Nanoparticles: In isopropanol (140mL), add tetraethyl orthosilicate (5mL), ultrapure water (11.6mL) and ammonia water (4.4mL) sequentially, and the resulting liquid mixture is stirred at room temperature for five hours, and then centrifuged Separation, washing with ethanol, and drying at 80 °C for 12 h afforded SiO 2 solid.

[0052] SiO 2 Synthesis of @Mn / Ni LDHs: SiO was added to 18 mL ultrapure water 2 Nanoparticles (80mg), Potassium Bromate (0.9g, 5.39mM), Acrylamide (0.8g, 11.25mM), NiCl 2 ·6H 2 O (0.12g, 0.5mM) and MnCl 2 4H 2 O (0.02g, 0.1mM), stirred at room temperature for 1 hour, sealed in an autoclave, reacted at 140°C for 10h, washed with water after centrifugation, and dried overnight at 80°C to obtain SiO 2 @Mn / Ni LDHs.

[0053] To prepare hollow Mn / Ni LDHs, the SiO 2 @Mn / Ni LDHs (30mg) were dispersed in 30mL NaOH s...

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Abstract

The invention belongs to the technical field of electrochemical biosensors, and particularly relates to an electrochemical biosensor for UDG detection and a detection method thereof. The electrochemical biosensor is obtained by co-incubation of hollow Mn / Ni layered double hydroxides loaded with gold nanoparticles, DNA1 and hairpin DNA. The electrochemical biosensor based on h-Mn / Ni LDHs has a cascade signal amplification function. The biosensor shows excellent analysis performance, and the detection limit is as low as 4.8 * 10 <-4 > U mL <-1 >. More importantly, the strategy provides a new way for the application of the oxidase-like enzyme in the electrochemical biosensor, and can be expanded to sensitively detect various biomarkers for biological analysis and clinical diagnosis.

Description

technical field [0001] The disclosure belongs to the technical field of electrochemical biosensors, in particular to an electrochemical biosensor for UDG detection and a detection method thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Nanozymes are nanomaterials with enzymatic activity. Because of their high stability, convenience in large-scale preparation, and flexibility in transformation, they have become powerful candidates to replace natural enzymes. Most of the nanozyme-based biosensors that have been successfully prepared so far focus on peroxidase-like enzymes, among which H 2 o 2 It is necessary and can be catalyzed to generate active oxygen to achiev...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/3278G01N27/3277
Inventor 张晓梅柳婷婷陈墨涵
Owner SHANDONG UNIV