A platinum dendritic nanoparticle for visual and rapid detection of ascorbic acid, its preparation method and application
A nanoparticle and ascorbic acid technology, applied in the field of materials, can solve problems such as difficulty in realizing Ce recovery, affecting detection stability, and low detection limit, and achieve excellent oxidase-like activity, simple preparation method, and good stability.
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Embodiment 1
[0063] A preparation process of platinum dendritic nanoparticles (Pt-Au DNPs) is as follows:
[0064] (1) In a 50mL three-neck flask, add 2.5mL of ethylene glycol, stir (at a speed of 300r / min), condense and reflux at an oil bath heating temperature of 180°C for 5min; (2) then add 0.35mL with a concentration of 35wt% Hydrochloric acid; (3) Then, add 1mL of precursor mixed solution (precursor mixed solution is made of H 2 PtCl 6 ·6H 2 O and HAuCl 4 4H 2 O solution composition, both concentrations are 0.06M, volume ratio: H 2 PtCl 6 ·6H 2 O:HAuCl 4 4H 2 O=5:1) and 4mL of PVP solution with a concentration of 0.04M, add 100 μL of the precursor mixture solution each time, add 400 μL of PVP each time, add once every 30s, and complete this process within 5min; (4) The reaction is maintained at 180 After 20 minutes of reaction, the solution turned black; (5) After the reaction, the three-neck flask was removed, cooled to room temperature naturally, acetone and ethanol were al...
Embodiment 2
[0067] Oxidase-like activity of platinum dendritic nanoparticles (Pt-Au DNPs) according to the present invention.
[0068] In order to explore the catalytic activity of the catalyst imitating enzymes, TMB was selected as the chromogenic substrate, and the experimental steps were as follows: 0.75 mg of platinum dendritic nanoparticles (Pt-Au DNPs) were ultrasonically dispersed in 1 mL of deionized water, and 60 μL (the control group did not To add the catalyst, take 60 μL of deionized water), add to 2900 μL of buffer solution (acetic acid-sodium acetate buffer solution (0.2M, pH=4.0)), and then add 40 μL of TMB (5 mM) ethanol solution thereto. React in a water bath at 37°C for 10 min, and then stand in an ice bath for 5 min to terminate the reaction. Finally, measure the absorbance value of the final solution at 400-800 nm. The result is as figure 2 , it can be seen that platinum dendritic nanoparticles (Pt-Au DNPs) catalyzed the oxidation of TMB to produce a typical blue pr...
Embodiment 3
[0070] Kinetic parameters of the platinum dendritic nanoparticle (Pt-Au DNPs) oxidation simulation enzyme described in the present invention.
[0071] Kinetic experiment: at 35°C, take 60μL 0.75mg mL -1Catalyst solution (that is, the dispersion liquid formed by ultrasonic dispersion of 0.75 mg of platinum dendritic nanoparticles in 1 mL of deionized water), 40 μL of TMB (5 mM), was added to 2.9 mL of acetic acid-acetate buffer solution (0.2 M, pH 4.0) . Kinetic measurements were performed in time course mode using a spectrophotometer to detect the absorbance of the reaction system at 652 nm every 15 s for the first 3 min. Such as image 3 As shown, Michaelis-Menten constants were calculated using the Lineweaver-Burk plot. The calculated Michaelis constant Km is 0.22mM, and the maximum reaction rate Vmax is 282nM S -1 , indicating that platinum dendritic nanoparticles (Pt-Au DNPs) have higher affinity and catalytic reaction rate to TMB, and have good catalytic activity like...
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