Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing manganese silicate nano-enzyme based on silicate dissolution kinetics and application

A technology of silicate and manganese silicate, which is applied in chemical instruments and methods, nanotechnology, and analysis through chemical reactions of materials to achieve high catalytic efficiency, high stability, and low cost

Active Publication Date: 2021-11-05
NANJING UNIV
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the preparation of manganese silicate based on natural silicate in the prior art

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing manganese silicate nano-enzyme based on silicate dissolution kinetics and application
  • Method for preparing manganese silicate nano-enzyme based on silicate dissolution kinetics and application
  • Method for preparing manganese silicate nano-enzyme based on silicate dissolution kinetics and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The method for preparing manganese silicate nanozyme based on silicate dissolution kinetics comprises the following steps:

[0041] S1: Physical preprocessing

[0042] Natural silicate minerals are ground and sieved to remove impurities to obtain high-purity mineral powders;

[0043] S2: Ultrasonic pretreatment

[0044] Disperse the high-purity mineral powder obtained in step S1 in water, and perform ultrasonic treatment under the condition of mechanical stirring to obtain a monodisperse silicate suspension;

[0045] S3: silicate dissolution

[0046] Adding an organic ligand to the monodisperse silicate suspension obtained in step S2, the organic ligand complexes the metal cations on the surface of the silicate mineral nanostructure, so that the natural silicate dissolves and forms SiO4-4 groups in situ , to obtain a solution containing SiO4-4 groups;

[0047] S4: Aging Response

[0048] In the containing SiO4-4 group solution that step S3 obtains, add ammoniacal l...

Embodiment 2

[0059] The difference between this embodiment and embodiment 1 is:

[0060] The natural silicate mineral in step S1 is attapulgite.

[0061] In step S1, the grinding speed is 100r / min, the grinding time is 1.5h, and the mesh size of the sieve for impurity removal is 200 mesh. Such powder has a good dispersion effect in deionized water.

[0062] The specific steps of ultrasonic pretreatment in step S2 are: disperse 0.2 g of the screened silicate mineral powder in 100 mL of deionized water, and use a 30 KHz ultrasonic cleaning machine to perform ultrasonic dispersion treatment for 0.5 h under mechanical stirring at 150 r / min. , under the above ultrasonic treatment parameters, the ultrasonic treatment effect is good and the efficiency is high.

[0063] The organic ligand in step S3 is sodium oxalate.

[0064] The catalytic metal manganese salt in step S4 is manganese acetate, and the above-mentioned manganese salt has a good catalytic effect.

[0065] After adding ammonia wate...

Embodiment 3

[0068] The difference between this embodiment and embodiment 1 is:

[0069] The natural silicate mineral in step S1 is vermiculite.

[0070] In step S1, the grinding speed is 110r / min, the grinding time is 1.5h, and the mesh size of the sieve for impurity removal is 300 mesh. Such powder has a good dispersion effect in deionized water.

[0071] The specific steps of ultrasonic pretreatment in step S2 are as follows: disperse 1 g of the screened silicate mineral powder in 100 mL of deionized water, and use a 30 KHz ultrasonic cleaning machine to perform ultrasonic dispersion treatment for 1 h under mechanical stirring at 200 r / min. Under the ultrasonic treatment parameters, the ultrasonic treatment effect is good and the efficiency is high.

[0072] The organic ligand of step S3 is tartaric acid.

[0073] The catalytic metal manganese salt in step S4 is manganese sulfate, and the above-mentioned manganese salt has a good catalytic effect.

[0074] After adding ammonia water / ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for preparing manganese silicate nano-enzyme based on silicate dissolution kinetics and application, and relates to the technical field of material chemistry and nano-enzyme catalyst. The method comprises the four steps of physical pretreatment, ultrasonic pretreatment, silicate dissolution and aging reaction. The method specifically comprises the following steps: taking natural silicate minerals as a template, after grinding, sieving and ultrasonic dispersion pretreatment, promoting partial dissolution of silicate by using an organic ligand and forming a SiO4-4 group in situ; and finally, catalyzing the combination of Mn < 2 + > and SiO4-4 groups by using an ammonia water / ammonium chloride buffer solution, and carrying out aging reaction to prepare the manganese silicate nano-enzyme. The manganese silicate nano-enzyme is used as mimic oxidase and can be used for detecting phenol compounds and catalytically oxidizing a horseradish peroxidase substrate by a 4-amino-amidepyrine colorimetric method. The manganese silicate nano-enzyme prepared by the method is complete in structure, low in synthesis cost and simple in process, has the advantages of economy and large-scale production, and has wide application prospects in the aspects of environment, biological catalytic oxidation and medical immunity.

Description

technical field [0001] The invention relates to the technical field of material chemistry and nanozyme catalysis, in particular to a method and application for preparing manganese silicate nanozyme based on silicate dissolution kinetics. Background technique [0002] Natural oxidases in organisms are the main enzymes in peroxisomes, accounting for about half of the total peroxisome enzymes, including glucose oxidase, uric acid oxidase, laccase, tyrosinase, etc. Essentially, various oxidases catalyze the oxidation of different substrates such as phenol, TMB or ABTS by generating water using molecular oxygen as an electron acceptor. However, natural oxidases are not reusable, sensitive to the environment, poor in stability and high in synthesis cost, which greatly limits their application in biotechnology and industrialization. [0003] As a nanomaterial with enzymatic activity and unique nanostructure, nanozyme is considered to be an excellent substitute for natural enzymes....

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C01B33/20B82Y40/00G01N21/31G01N21/78
CPCC01B33/20B82Y40/00G01N21/78G01N21/31C01P2002/72C01P2004/04C01P2002/84
Inventor 吕路王伟伟魏蕾张炜铭潘丙才
Owner NANJING UNIV