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FOXM1 antagonistic polypeptide as well as derivative and application thereof

A derivative and antagonistic technology, applied in the fields of biotechnology and biomedicine, can solve the problems of anti-tumor drugs on the market, and achieve the effect of inhibiting the proliferation of cancer cells, promoting apoptosis, and huge social and economic benefits.

Active Publication Date: 2021-12-10
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, domestic FOXM1-targeted drug therapy is still in the preclinical research stage, and no anti-tumor drug directly targeting FOXM1 has been marketed or applied in clinical practice.

Method used

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  • FOXM1 antagonistic polypeptide as well as derivative and application thereof
  • FOXM1 antagonistic polypeptide as well as derivative and application thereof
  • FOXM1 antagonistic polypeptide as well as derivative and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1F

[0037] Example 1FIP-1 can be combined with FOXM1 in HepG2 cells

[0038] The HepG2 cells having good state were seeded in a 12-well plate containing the climbing, and fresh medium was added to culture overnight, and the labeled polypeptide, FIP-1 and control group polypeptide TAT were added the next morning, and the culture plate was cultured. The slides that have been climbed in the cells were dipped 3 times with PBS, 3 min each time; 4% polymethyledealdehyde fixed climb for 15 min, PBS dipped 3 times, 3 min each time; 0.5% Triton X-100 (PBS preparation) Room temperature Transparent 20min (antigen expressed in the cell membrane omitted this step); PBS dipped 3 times, 3 min, water absorbing paper to dry PBS, add normal goat blood on a slide, room temperature to close 30 min; The water absorbent is suiled to remove the enclosure, not wash, each slide is dropped with a sufficient amount of diluted one-resistant and placed in a wet cartridge, incubate overnight at 4 ° C; second day p...

Embodiment 2F

[0039] Example 2FIP-1 can inhibit the proliferation of HepG2 cells

[0040] The heporous cell hepg2 was seeded in a 6-well cell culture plate in 100 / well, and the medium per well was 2 mL, and 24 h was cultured. The medium and drugs were replaced every two days, continuously cultured, and the cell group contains more than 50 cells, and 0.1% crystalline purple color, statistically, the number of cloned formations in each group was carried out with methanol fixed experimental group and control group. image 3 It can be seen from the figure that FIP-1 can effectively inhibit the number of cells of the cells, inhibit the formation of clones, confirming that FIP-1 can effectively inhibit cell proliferation.

Embodiment 3

[0041] Example 3CCK-8 detection of inhibitory effects of FIP-1 on HepG2 cells

[0042] In a 10 cm culture medium inoculated in a 10 cm culture medium, it was cultured to 95% by 10% FBS DMEM medium. After the cells reached the desired density, the medium was removed, washed twice with PBS, add 1 ml of trypsin, and incubate 37 degrees. After 1 min, 1 ml of 10% FBS DMEM medium was added, and the dispersion cell was tapped, and 10 μl of cell suspension was added to 1 ml of DMEM medium to prepare cell suspension, and cells prepared by cells were counted. Quantity, then press each hole 5 × 10 3 The cultured in 96-well plates for 24 hours. On the second day, the cell culture medium was abandoned, and fresh medium and different concentrations of FIP-1 were added to the culture plate, and the culture plate was incubated for 48 hours in the incubator, and 10 μl of CCK-8 solution was added to each well (note not to be generated in the well. Bubbles, they affect the OD value of the OD value t...

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PUM

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Abstract

The invention discloses an FOXM1 antagonistic polypeptide as well as a derivative and application thereof. The amino acid sequence of the FOXM1 antagonistic polypeptide is shown as SEQ ID No: 1. The antagonistic polypeptide and the derivative thereof can be combined with FOXM1, a downstream signal channel is blocked through combination with the FOXM1, so that liver cancer cell proliferation is inhibited, liver cancer cell apoptosis is promoted, effective small molecule drugs for treating liver cancer and the like are provided, and the antagonistic polypeptide and the derivative thereof can be widely applied to the fields of medicines and biology.

Description

Technical field [0001] The invention belongs to biotechnology and medical technology relates to the field FOXM1 polypeptide antagonist and derivatives thereof and application. Background technique [0002] Hepatocellular carcinoma (HCC) is one of the most common malignancies in the world. World Health Organization International Center for Research on Cancer (International Agency for Research on Cancer, IARC) report notes that ranks fifth in cancer located after lung cancer, breast cancer, liver cancer incidence, mortality rate of lung cancer is located, after colorectal, cancer ranks first 3, the top five highest rates of cancer death in the only annual increase in incidence of cancer. Geographical distribution of the incidence of liver cancer in the world there are significant differences, the higher the incidence of liver disease in developing countries. Risk factors include hepatitis B virus, hepatitis C virus, fatty liver, alcohol-related cirrhosis of the liver, smoking, obes...

Claims

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Application Information

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IPC IPC(8): C07K7/08C12N15/11A61K38/10A61P35/00G01N33/574G01N33/68
CPCC07K7/08A61P35/00G01N33/57484G01N33/6893A61K38/00
Inventor 黄来强王坤代小勇冯春燕贝利过冬冬胡洋
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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