Pan.vagans capable of efficiently antagonizing penicillium and botrytis cinerea and application of Pan.vagans in kiwi fruit storage
A bio-control bacteria and Penicillium technology, applied in the field of agricultural product storage, can solve the problems of easy residues, drug-resistant strains, weak inherent flavor, etc., and achieve the effects of prolonging storage time and efficient antagonism
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Embodiment 1
[0030] Example 1: A highly effective biocontrol bacterium against Penicillium and Botrytis cinerea and its application in kiwifruit storage
[0031] A biocontrol bacterium Pan.vagans BWL1 that is highly effective against Penicillium and Botrytis cinerea was preserved in the China Center for Type Culture Collection on January 4, 2021. Address: Wuhan, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province, China In the university campus, the deposit number is CCTCC NO: M2021001. The biocontrol bacterium Pan.vagans BWL1 is isolated from the surface of healthy citrus fruits. It is a resident bacterium of fruit. Using the plate confrontation method, it was found that the biocontrol bacterium Pan.vagans BWL1 has inhibitory effects on both Penicillium and Botrytis cinerea. The isolation, screening and identification methods of the biocontrol bacteria Pan.vagans BWL1 are as follows.
[0032] (1) Isolation of antagonistic bacteria
[0033] Take commercially available healthy...
Embodiment 2
[0085] Example 2: A highly effective biocontrol bacterium against Penicillium and Botrytis and its application in kiwifruit storage
[0086] A biocontrol bacterium Pan.vagans BWL1 that is highly effective against Penicillium and Botrytis cinerea was deposited in the China Center for Type Culture Collection (CCTCC) on January 4, 2021, with the preservation number CCTCC NO: M2021001.
[0087] A microbial preparation of the biocontrol bacterium Pan.vagans BWL1, characterized in that: the number of live bacteria of the biocontrol bacterium Pan.vagans BWL1 of the microbial preparation of the biocontrol bacterium Pan.vagansBWL1 is greater than or equal to 10 8 CFU / mL.
[0088] The preferred technical scheme is: the preparation method comprises:
[0089] Step 1: Inoculate the biocontrol bacterium Pan.vagans BWL1 in LB medium and culture it in a shaker at 23°C for 18 hours;
[0090] Step 2: Inoculate the bacterial liquid obtained in step 1 into a new LB medium for expanded culture a...
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