Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of urine ankle protein 1 and polypeptide fragment thereof in burns

A polypeptide fragment, talin technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, material inspection products, etc., can solve the problems of poor skin integrity, aggravate the pain of patients, etc., and achieve the effect of convenient storage.

Pending Publication Date: 2021-12-10
BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the integrity of the skin of patients with severe burns is poor. As an invasive skin test, there are many difficulties in the clinical application of blood tests for such patients. Repeated blood testing will also aggravate the suffering of patients.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of urine ankle protein 1 and polypeptide fragment thereof in burns
  • Application of urine ankle protein 1 and polypeptide fragment thereof in burns
  • Application of urine ankle protein 1 and polypeptide fragment thereof in burns

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Collection and processing of urine specimens

[0022] The burn patients were selected as the burn group, and the healthy subjects during the same period were selected as the normal control group. 30ml fresh morning urine samples were collected from the subjects in each group after admission. Those who could not urinate normally were collected from their morning catheters and placed in a dry and clean container. After the collected urine samples were centrifuged at 4000 r / min for 5 min, the supernatant was aspirated, and each tube was divided into 2 ml and stored in a -80 °C refrigerator.

Embodiment 2

[0023] Example 2 Mass Spectrometry and Screening of Urine Peptides

[0024] Urine samples were extracted for protein and the concentration of the extracted protein was determined. Mass spectrometry analysis of urine samples was performed by OrbitrapFusion mass spectrometry. The data obtained in the mass spectrometry of the experimental group and the normal control group were quantitatively calculated. Differences between groups were analyzed by t-test, and the differentially expressed proteins were screened with the difference in protein expression more than 1.5 times and the statistical test P<0.05 as the reference standard.

Embodiment 3

[0025] Example 3 Identification and Analysis of Differential Peptides

[0026] The database used was Uniprot_Homo database, and the original mass spectrum files were processed by MaxQuant software. See Table 1 for search parameter settings.

[0027]

[0028] Compared with healthy people, talin 1 is highly expressed in the urine of burn patients, such as figure 1 As shown, the main biological processes it participates in are as follows figure 2 As shown, the expression of talin 1 in the urine of the normal control group and the burn group was significantly different.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of a urine ankle protein 1 (Talin-1) and a polypeptide fragment thereof, and particularly relates to application of the urine ankle protein 1 and the polypeptide fragment thereof in preparing preparations for burn diagnosis, differential diagnosis, burn area and degree evaluation, treatment effect evaluation, monitoring, prognosis evaluation, mechanism research and the like. Burns are common important wounds in daily life, about 5,000-100,000 people in every one million people are burnt every year, according to statistics of World Health Organization, more than 300,000 patients die from burns globally every year, and the treatment survival rate of seriously burnt patients is still at a lower level. Researches prove that compared with healthy people (a normal control group), the expression of the urine ankle protein 1 and the polypeptide fragment thereof in burn patients is increased, and the urine ankle protein 1 and the polypeptide fragment thereof can be used for various purposes of application detection of burn patients. The advantages of noninvasive acquisition, large-scale repeated sampling and convenient preservation of a urine sample are exerted, and the urine sample is used for detecting the urine ankle protein 1 and the polypeptide fragment thereof.

Description

technical field [0001] The invention relates to new uses of urine talin 1 and its polypeptide fragments, in particular to urine talin 1 and its polypeptide fragments in burn diagnosis, differential diagnosis, burn area and degree evaluation, treatment effect evaluation, monitoring, prognosis evaluation and mechanism applications for research, etc. Background technique [0002] Burn refers to the damage of human skin, tissue and even deep organs caused by chemical substances such as flame, high temperature gas, hot solid or liquid, radiation, electricity, strong acid and alkali. It is a systemic comprehensive disease. Mass necrosis, infection, shock and coagulation dysfunction of wound tissue after burn can cause a series of pathophysiological changes in the body. Different degrees of burns have different effects on the human body. Severe burns can cause damage to the internal environment of the human body, causing burn patients to experience complex pathophysiological chang...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/543C12Q1/6883
CPCG01N33/6893G01N33/54306G01N33/6848C12Q1/6883C12Q2600/158G01N2800/52G01N2800/40G01N2333/47
Inventor 张曼王佶图
Owner BEIJING SHIJITAN HOSPITAL CAPITAL MEDICAL UNIVERSTY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com