Growth stimulation expression factor 2 instant detection kit, and preparation method and application thereof

Abstract

The invention relates to a growth stimulus expression factor 2 instant detection kit and a preparation method and application thereof, and the instant detection kit comprises an alkaline phosphatase labeled anti-growth stimulus expression factor 2 antibody working solution, a hydroxyl magnetic bead coated anti-growth stimulus expression factor 2 antibody working solution, a luminescent substrate working solution and a working cleaning solution; the luminous substrate working solution comprises a substrate solvent and 0.5% bovine serum albumin; the substrate solvent comprises the following components: 40% of diethanol amine, 30% of a 1M sodium hydroxide solution, 10% of a 0.1 M magnesium chloride solution, 10% of a 0.25% Proclin300 solution, 5% of a 0.5% gemini surfactant solution and 5% of AMPPD. The surface arrangement of the gemini surfactant solution is tighter, the surface energy is lower, the gemini surfactant solution has higher surface activity, the solubility of AMPPD in distilled water can be improved, the chemiluminescence reaction efficiency is promoted, and lower critical micelle concentration is achieved. The bovine serum albumin and the gemini surfactant are matched for use, so that the luminosity can be remarkably enhanced, and the detection sensitivity is further remarkably enhanced.

Description

technical field [0001] The invention relates to the technical field of kits, in particular to a growth-stimulating expression factor 2 instant detection kit, its preparation method and application. Background technique [0002] Heart failure, referred to as heart failure, refers to the failure of the venous return blood to fully discharge the heart due to the dysfunction of the systolic function and / or diastolic function of the heart, resulting in blood stasis in the venous system and insufficient blood perfusion in the arterial system, resulting in cardiac circulation syndrome. The cluster of symptoms of this disorder manifests as pulmonary congestion and vena cava congestion. The diagnosis of heart failure is mainly based on clinical manifestations. However, clinical heart failure may be difficult to diagnose due to mild symptoms, unobvious signs caused by obesity, and pulmonary diseases. In addition, there is a lack of laboratory reference indicators with high sensitivit...

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Problems solved by technology

[0005] In order to solve the problem of low sensitivity when the traditional growth-stimulating expression factor 2 detection kit is used to detect the content of growth-sti

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