Absolute value counting method for obtaining number of trace cells in cell suspension
A cell suspension and micro-cell technology, which is applied in the field of biomedicine, can solve the problems of cell total deviation, large deviation, and uneven distribution of cells, and achieve the effect of eliminating counting deviation
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Embodiment 1
[0046] Before step 1, that is, before using a pipette to take the cell suspension, configure the cell suspension, use the cell diluent to dilute the cell mixture to be counted and mix to obtain the cell suspension. The cell mixture can be selected from Coagulated whole blood, filtered white blood or other cell culture fluid containing a small amount of cells, the cell diluent can be selected from physiological saline and phosphate buffered saline (PBS), the cell mixture used in Example 1 It is anticoagulated whole blood (low white blood cell content), and the cell diluent is physiological saline, which is obtained by diluting anticoagulant whole blood with normal saline, and the diluted cell suspension is mixed with fluorescent dye. Then take the mixed solution of cell suspension and fluorescent dye and add it to the counting plate. The volume of the diluted cell suspension is the same as that of the fluorescent dye and the mixed solution. After standing for a preset time, star...
Embodiment 2
[0049] Before step 1, before the cell suspension was taken with a pipette, the cell suspension was configured, and the cell mixture was directly used to configure the cell suspension. Therefore, in Example 2, the cell mixture was filtered white blood (leukocytes) low content), there is no need to prepare cell diluent for dilution, take the cell suspension (filtered blood) and the fluorescent dye in equal amounts and mix them evenly, add the mixture of the cell suspension and the fluorescent dye to the counting plate, and let it stand After the preset time, counting starts.
[0050] The specific implementation method is as follows, take 15 μl of cell suspension and 15 μl of fluorescent dye to mix and stir evenly, take 15 μl of the mixed liquid with a pipette and add it dropwise to the counting plate (such as figure 1 and figure 2 shown), let stand for 1min, start counting, and use the image processing system (camera with fluorescent signal capture) of the automatic fluorescen...
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