Monoclonal antibody targeting human Claudin18.2 protein and application thereof

Abstract

The invention relates to the technical field of biological antibodies, and particularly relates to a monoclonal antibody targeting human Claudin18.2 protein and application thereof. The monoclonal antibody comprises a heavy chain variable region HV and a light chain variable region LV, and the amino acid sequence and the nucleic acid sequence of the monoclonal antibody can be used in a single-chain mode or a double-chain combination mode. The monoclonal antibody can specifically recognize the human Claudin18.2 antigen, does not recognize the human Claudin18.1 antigen, is good in specificity in human normal tissue, only combines with Claudin18.2 positive tissue, does not recognize other tissue organs and has no off-target risk. The monoclonal antibody has a specific antibody mediated complement-dependent (CDC) killing effect on Claudin18.2 positive target cells, and CAR-T prepared from the monoclonal antibody has a specific killing function on the Claudin18.2 positive target cells.

Description

technical field [0001] The present invention relates to the technical field of biological cells, in particular to a monoclonal antibody targeting human Claudin18.2 protein, which can be used as a biological material for the construction of other DNA vectors, expression of recombinant proteins, construction of cell lines and chimeric antigen receptors In immune cells; or used as a biological agent after coupling with other substances, or used directly as a biological agent. Background technique [0002] Claudins are a family of proteins whose role is to maintain the tight junctions that control the exchange of molecules between cells. They are widely distributed in stomach, pancreas and lung tissues and can be used for disease diagnosis and treatment. CLDN18.2 (i.e., Claudin 18.2) isoform as a gastric-specific isoform has become an ideal since Sahin found that CLDN18.2 is a highly selective molecule and is widely expressed only in cancer cells. target. CLDN18.2 is usually b...

AI Technical Summary

Problems solved by technology

The expression distributions of Claudin 18.1 and Claudin 18.2 are different, Claudin18.1 is selectively expressed in normal lung cells, and Claudin 18.2 is highly restricted in normal cells, but in a variety of tumors (such as gastric cancer, lung cancer and pancreatic cancer frequent ectopic activation and overexpression in

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